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- 저자 : Aditi Kundu (검색결과 2 건)
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Anti-nemic secondary metabolites produced by Fusarium oxysporum f. sp. ciceris
Aditi Kundu,Supradip Saha,SureshWalia,Tushar Kanti Dutta 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Several nonpathogenic strains of Fusarium oxysporum have been selected as potential biological control agents. Culture filtrate of F. oxysporum f. sp. ciceris was extracted sequentially with ethyl acetate (EtOAc) and n-butanol (n-BuOH). Chromatographic separation of EtOAc concentrate has afforded five compounds, bikaverin (1), 3-O-methyl-8-O-methyl fusarubin (2), 8-O-methyl fusarubin (3), anhydrofusarubin (4) and fusarubin (5). Structures of these compounds were elucidated by detailed NMR and LC/ESI-MS spectra. Anti-nemic activity of the extracts and secondary metabolites were evaluated against root-knot nematode, Meloidogyne incognita and reniform nematode, Rotylenchulus reniformis. EtOAc concentrate was found to be strongly active against M. incognita (LC50 56.2 μgmL−1) followed by n-BuOH concentrate (LC50 97.4 μgmL−1). However, these concentrates were moderately effective on R. reniformis (LC50 134.5–189.2 μg mL−1). Fusarubin (LC50 248.9 μg mL−1) was found to be highly active followed by anhydrofusarubin (LC50 257.6 μgmL−1) againstM. incognita. Structure anti-nemic activity relationship indicated comparatively more polar molecule with naphthoquinone nucleus as most active. Fusarubin analogues could be exploited further to develop standard nematicidal liquid formulation.
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Ahmad Ayashaa,Kundu Aditi,Dey Debjani 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2
Droschia stebbingii (Green) belonging to the family Monophlebidae (Hemiptera: Coccoidea) is a serious, phytophagous and dimorphic pest. D. stebbingii and D. mangiferae (Green) with sixty-two common hosts and minor morphological variations pose problems towards their taxonomic identification. Light and scanning electron microscopy were used to outline the ultrastructural details of cuticular wax producing structures of D. stebbingii. Three types of wax-pores along with tubular duct were observed on the head, thorax and abdomen. Quinquelocular and multilocular disc-pores exhibited variations in the central loculus with single and double opening. Short curly wax strands were secreted from the outer loculi whereas central loculus secreted long tubular strands. Additionally, bilocular pores secreting curly wax were observed having two large loculi separated by a septum. GC–MS analysis of cuticular wax indicated the presence of hydrocarbons and wax esters forming majority of the covering along with long chain alcohols, ketones and acids. Variations in the percentage of hydrocarbon and wax esters content were observed for two populations of D. stebbingii collected from different hosts. Hydrocarbons were abundant in populations collected on Pongamia pinnata (L.) Pierre whereas long chain esters dominated the wax profile for the population collected from Tectona grandis L.f. Compositional variations in the cuticular wax of D. stebbingii on different host along with knowledge on the ultrastructural wax pores and waxy secretion would provide an insight to the existing knowledge of this scale insect.
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