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        Extraction of high thermally stable and nanofibrous chitin from Cicada (Cicadoidea)

        Abbas Mol,Murat Kaya,Muhammad Mujtaba,Bahar AKYUZ 한국곤충학회 2018 Entomological Research Vol.48 No.6

        Due to the increasing interest in natural biopolymers including chitin, the exploitation of economic and easily accessible chitin sources with good physicochemical properties is nowadays required. In view of this fact, in the current study chitin was extracted and physicochemically characterized from six Cicadas (Hemiptera: Homoptera: Cicadoidea) species collected from Mediterranean region of Turkey (2014–15). Chitin was extracted using a classic extraction method that includes acid and base treatment. TGA results revealed a remarkable increase (410–412°C) for all the six Cicada species compared to other chitin samples extracted from various sources. For all of the six selected species the chitin contents on the dry basis were determined as 6.7% for Cicadatra atra, 5.51% for C. hyalina, 8.84% for C. platyptera, 4.97% for Cicada lodosi, 6.49% for C. mordoganensis, and 5.88% for Cicadetta tibialis. The surface morphology of chitin isolates from Cicada species was observed to consist of nanofibers and nanopores.

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        Extraction and Characterization of Chitin and Chitosan with Antimicrobial and Antioxidant Activities from Cosmopolitan Orthoptera Species (Insecta

        Murat Kaya,Talat Baran,Meltem Asan-Ozusaglam,Yavuz Selim Cakmak,Kabil Ozcan Tozak,Abbas Mol,Ayfer Mentes,Goksal Sezen 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

        This study examined two gregarious Orthopteraspecies (Calliptamus barbarus and Oedaleus decorus) aspotential sources of chitin. The chitin content of the dryweight of C. barbarus was 20.5 ± 0.7%, and it was 16.5 ±0.7% for O. decorus. Furthermore, the yield of chitosan (70~ 75% deacetylation degree) from the grasshopper specieswas found to be 74 ~ 76%, which is close to the yield ofcommercial preparations obtained from the unused parts ofcrabs and shrimp. The chitin and chitosan obtained in thisway were analyzed using FTIR, TGA, XRD and SEMtechniques, and the antimicrobial properties of chitosansobtained from C. barbarus and O. decorus against pathogenicmicroorganisms of humans and fish were investigatedusing the disc diffusion and microdilution broth methods. The antimicrobial screening procedures indicated that thechitosan showed significant antimicrobial activity againstall of the tested pathogenic microorganisms. The MBC orMFC values were determined to be 0.16 ~ 2.50 mg/mL. The IC50 values for the chitins obtained from C. barbarusand O. decorus were 10.68 ± 0.27 and 10.91 ± 0.96 mg/mL,respectively, which were greater than the value forbutylated hydroxytoluene (BHT): 0.04 ± 0.01 mg/mL. These results suggest that these two species, which arecurrently considered to be pests because of over-breeding,are potentially alternative sources of chitin and chitosan,which are used in the food/feed industry for theirantimicrobial and antioxidant properties.

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