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공초점레이저주사현미경을 이용한 일부 시판음료에 의한 정상법 랑질의 치아부식도 평가
윤혜정 ( Hye Jeong Youn ),홍석진 ( Suk Jin Hong ),정성숙 ( Seong Soog Jeong ),정은주 ( Eun Ju Jung ),하명옥 ( Myung Ok Ha ),박영남 ( Young Nam Park ),최충호 ( Choong Ho Choi ) 대한예방치과·구강보건학회 2011 大韓口腔保健學會誌 Vol.35 No.3
Objectives. The purpose of this study was to evaluate the effect of dental erosion by some commercial beverages on sound enamel of bovine teeth using confocal laser scanning microscopy (CLSM). Methods. Three experimental groups (mixed beverage, carbonated flavored, and fruit juice) and one control group (natural mineral water) were established for this study. Each group consisted of 11 specimens obtained from bovine teeth. The concentration, pH level, fluoride, Ca and P of beverages were measured. Specimens were immersed in artificial saliva, except beverage treatment for day 8, and surface microhardness and lesion depth were measured. Results. The pH level of the four beverages was in the range of 2.39∼7.60. The concentration levels of fluoride, Ca and P were in the range of 0.03∼0.16 ppm, 1.67∼9.07 mg per 100 gm, and 0∼3.73 mg per 100 gm, respectively. The differences in surface microhardness (VHN) before and after the 8 day treatment were significant among the four groups for sound enamel. Lesion depths after the 8 day treatment on sound enamel by CLSM decreased significantly in of the following order: mixed beverage, carbonated flavored, fruit juice and natural mineral water (p<0.01). Lesion depths by CLSM correlated with surface microhardness using the surface hardness test on sound enamel (r=?0.920). Conclusions. In this study, beverages with rich Ca and low pH showed lower VHN and lesion depth. Thus, we propose that low pH beverages with rich Ca may reduce the extent of dental erosion on sound enamel.
CaCl<sub>2</sub> 용융염에서 TiO<sub>2</sub> 펠렛의 전기화학적 환원반응 특성
지현섭,류효열,정하명,정광호,정상문,Ji, Hyun-Sub,Ryu, Hyo-Yeol,Jeong, Ha-Myung,Jeong, Kwang-Ho,Jeong, Sang-Mun 한국방사성폐기물학회 2012 방사성폐기물학회지 Vol.10 No.2
본 연구에서는 $850^{\circ}C$의 $CaCl_2$ 용융염계에서 전해환원공정을 통해 $TiO_2$로부터 금속티타늄을 제조하였다. Ni-$TiO_2$ 조합전극을 환원전극으로 그라파이트를 산화전극으로 사용하였으며, 셀전위를 제어하면서 $TiO_2$의 전해환원 특성을 관찰하였다. XRD 분석을 통해 $TiO_2$가 $CaTiO_3$, $Ti_2O$, $Ti_6O$와 같은 다양한 반응 중간생성물을 거쳐 Ti 스폰지로 환원되는 것이 밝혀졌다. 또한 SEM 분석을 통해 $TiO_2$ 전해환원 반응동안 펠렛의 바깥표면부터 환원반응이 시작되어 펠렛중심으로 진행이 되는 것이 확인되었다. 전해환원 반응도중 환원된 티타늄금속은 초기에는 다공성 스폰지 구조를 보이나 고온에서 반응이 진행됨에 따라 점차 소결에 의해 수축되어 다공성 구조가 사라지는 현상을 보였다. A porous $TiO_2$ pellet was electrochemically converted to the metallic titanium by using a $CaCl_2$ molten salt system at $850^{\circ}C$. Ni-$TiO_2$ and graphite electrodes were used as cathode and anode, respectively. The electrochemical behaviour of $TiO_2$ pellet was determined by a constant voltage control electrolysis. Various reaction intermediates such as $CaTiO_3$, $Ti_2O$ and $Ti_6O$ were observed by XRD analysis during electrolysis of the pellet. Once $TiO_2$ pellet was converted to a porous metallic structure, the porous structure disappeared by sintering and shrinking with increasing the reaction time at high temperature.
인공치면세균막 제거 방법에 의한 RBM 처리 임플란트의 표면거칠기 변화
오희균 ( Hee Kyun Oh ),하명옥 ( Myung Ok Ha ),정성숙 ( Seong Soog Jeong ),정은주 ( Eun Ju Jung ),최충호 ( Choong Ho Choi ),홍석진 ( Suk Jin Hong ) 대한예방치과·구강보건학회 2009 大韓口腔保健學會誌 Vol.33 No.2
Objective: The objective of this study was to evaluate the change in the surface roughness that is being produced by physical or chemical materials for removing of dental plaque from the surface of an RBM(resorbable blasting media)- treated implant. Methods: The RBM treated implant discs were used after artificial plaque was formed by Streptococcus mutans. This study is composed of one control, three physical and three chemical methods that were used to remove dental plaque. The physical methods applied on the RBM implant were prophyflex, professional mechanical tooth cleaning and interdental brushing. The chemicals methods applied to remove plaque on the RBM implants were 0.1% chlorhexidine, citric acid and HCL tetracycline. Each disc was sonically cleaned, air dried, and measured by a profilemeter and a scanning electronic microscope (SEM). Results: There were no statistically significant differences between the control group (1.671±0.11) and each of the other groups (physical: 1.614±0.12, chemical: 1.624±0.09) in the surface roughness values (Ra:μm) after the methods of oral hygiene treatment (p>0.05) have been applied. SEM analysis showed that after each of the oral hygiene treatment methods, all of the groups looked like the original RBM implant surface as hydroxyapatite particles with irregular shapes and sizes were adhered to the surface of the RBM implants. Conclusion: The RBM implant surface was not affected by the six cleaning methods used in this study. These methods are all able to safely remove the plaque or calculus on RBM implants.
공초점 레이저 주사현미경을 이용한 죽염함유 불소치약의 초기우식법랑질 재광화 효과 연구
홍석진 ( Suk Jin Hong ),하명옥 ( Myung Ok Ha ),윤혜정 ( Hye Jeong Youn ),정성숙 ( Seong Soog Jeong ),정은주 ( Eun Ju Jung ),최충호 ( Choong Ho Choi ),오한나 ( Han Na Oh ),박영남 ( Young Nam Park ),임회순 ( Hoi Soon Lim ) 대한예방치과·구강보건학회 2009 大韓口腔保健學會誌 Vol.33 No.2
Objective: The purpose of this study was to investigate the remineralization effect of bamboo salt containing dentifrice on incipient dental caries lesions using confocal laser scanning microscope by the chemical pH cycling model. Methods: Specimens of bovine tooth enamel were embedded in resin, polished, exposed to a lactate carbopol buffer system for 48 hours, and divided into 3 groups: (1)control, (2)containing SMFP(0.76% and bamboo salt 2.0% with DCPD abrasive formula), and (3)containing NaF(0.22% and bamboo salt 3.0% with a silica abrasive formula). Each group contained 12 specimens. Surface hardness was compared before and after application of the chemical pH cycling sequences for 20 days. A confocal laser scanning microscope(CLSM) was used for evaluation of remineralization on subsurfaces of enamel lesions. Results: Remineralization of bamboo salt dentifrice with NaF, silica and bamboo salt 3% was significantly greater than those for the SMFP, DCPD and bamboo salt 2% groups as indicated by surface hardness (p<0.05). Dentifrice with NaF, silica and bamboo salt 3% decreased the fluorescent lesion depth compared to the other groups by CLSM. Conclusion: Dentifrice with NaF, silica and bamboo salt 3% could be a useful preventive agent against incipient dental caries.
뽕잎 추출물의 Streptococcus mutans에 대한 성장 억제 효과
정은주 ( Eun Ju Jung ),최충호 ( Choong Ho Choi ),최정이 ( Jeong Iee Choi ),이정근 ( Jeong Keun Lee ),정성숙 ( Seong Soog Jeong ),하명옥 ( Myung Ok Ha ),박영남 ( Young Nam Park ),홍석진 ( Suk Jin Hong ) 대한예방치과·구강보건학회 2012 大韓口腔保健學會誌 Vol.36 No.1
연구목적: 본 연구는 뽕잎(mulberry leaf) 추출물이 치아우식증의 주원인 균인 Streptococcus mutans의 성장억제에 미치는 영향을 알아보고자 시행하였다. 연구방법: 추출물의 세균 성장 억제 효과는 평판희석법을 시행하여 추출물의 농도(5, 20, 50 mg/mL)에 따라시간별(배양 직후, 6, 12, 24시간)로 Streptococcus mutans를 배양한 후 생균수를 측정하여 평가하였다. 또한 생약 추출물이 구강 내에서 안전하게 사용되기 위해서는 구강내 정상 연조직 세포에 독성이 없어야 하므로 사람의 정상 치은섬유아세포에 0, 5, 20, 50 mg/mL의 추출물을 처리하여 MTT로 세포독성을 검사하였다. 연구결과: 뽕잎 추출물의 농도에 따른 시간별 생균수 측정 결과, 생균수는 시간의 변화에 따라 통계적으로 유의하게 변화하였으며, 농도에 따라 생균수에 유의한 변화가 있었다(P<0.05). 추출물의 각 농도(5, 20, 50mg/mL)에서 88% 이상의 높은 세균 성장 억제율을 나타내었다. 정상 치은섬유아세포에 뽕잎 추출물을 처리하여 독성 유무를 측정한 결과, 20 mg/mL 이상의 농도에서 세포독성을 나타내었다. 결론: 뽕잎 추출물은 시간의 변화에 따라 생균수를 감소시켰으며, 대조군과 비교하여 높은 성장 억제율을 보여 억제능이 뛰어난 것으로 나타났다. 따라서 뽕잎 추출물은 다양한 우식 예방제재로서 사용될 수 있을 것으로 생각되었다.