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감성돔(Acanthopagrus schlegelii) 치어의 포르말린에 대한 내성 및 조직학적 반응
명정인 ( Jeong In Myeong ),민병화 ( Byunng Hwa Min ),박미선 ( Mi Seon Park ),황형규 ( Hyung Kyu Hwang ),도정완 ( Jeung Wan Do ),정경일 ( Kyung Il Jeoung ),장영진 ( Young Jin Chang ),정달상 ( Dal Sang Jeong ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.6
Black seabream juveniles Acanthopagrus schlegelii held at 20°C were exposed to formalin at concentrations of 50 to 400 ppm, and tolerance values were determined by calculating median lethal concentration values (LC50) through probit analysis. The 12-, 24,48, 72- and 96-h LC50 values for formalin were 297, 233, 171, 162 and 157 ppm, respectively. The histological effects of formalin on gill and liver tissues in this fish were determined. No histological effects were observed in the control group. The intensity of cell damage increased with the concentration of, and duration of exposure to, formalin. Hyperplasia, separation and epithelial necrosis,epithelial lifting, lamellar synechiae and collapsed lamellae were observed in gill tissues exposed to formalin. Hepatic lesions in liver tissues of fishes exposed to formalin were characterized by cloudy swelling of hepatocytes, necrosis, cytoplasmic vacuolization,deposition of pigments, spongiosis hepatis, nuclear hypertrophy, dilation of sinusoids and bile stagnation. The LC50 values and histological results obtained in this study will aid in designing treatment regimens to minimize toxic side effects and increase efficacy.