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방사선 조사 인삼이 랫드의 수태능 및 일반 생식독성에 미치는 영향에 관한 연구
박귀례(Kui Lea Park),한순영(Soon-Young Han),김판기(Pan Gyi Kim),이유미(You-Mie Lee),신재호(Jae-Ho Shin),장성재(Seung Jae Jang) 한국독성학회 2001 Toxicological Research Vol.17 No.2
Korean ginseng products have been fumigated with ethylene oxide (EO) for sterilization and prolongation of storage periods. However, there had been controversies indicating that the consumption of food treated with EO might cause harmful effects in human. Since, in Korea the use of EO gas for food treatment was banned in 1991. Since then, irradiation technique has been developed as an alternative. This study was carried out to investigate the effects of irradiated ginseng on fertility, and reproductive and developmental toxicity. Either EO gas fumigated or gamma-irradiated ginseng was administered to male rats by oral gavage for 63 days during the premating period. Female rats were administered from 14 days before mating to day 20 of gestation or to day 21 of lactation. The exposure amount of irradiation used was 5, 10 and 30 kGy, respectively. There were no treatment related changes of dams in clinical signs, and parturition. No treatment related changes in food consumption, body/organ weights, male/female reproductive and fertility performances were observed. F1 fetuses showed no external abnormality. Reflex/sensory functions, physical/behavioral development, and reproductive performance of F1 rats were not adversary affected. The results of this study show that gamma-irradiated ginseng, up to 30 kGy, has no adverse effects on the fertility, reproduction and development in Wistar rats.
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컴퓨터단층촬영으로 측정한 복강내 지방조직 길이와 내장지방 면적, 내장지방/피하지방 면적비 및 내장지방/골격근 면적비와의 비교
김수경 ( Soo Kyung Kim ),김세화 ( Se Hwa Kim ),이유미 ( Yu Mie Rhee ),최성희 ( Seung Hee Choi ),조유리 ( You Lee Cho ),안철우 ( Chul Woo Ahn ),차봉수 ( Bong Soo Cha ),임승길 ( Sung Kil Lim ),김경래 ( Kyung Rae Kim ),이현철 ( Hyun 대한내과학회 2003 대한내과학회지 Vol.64 No.1
배경 : 내장지방의 과잉 축적은 대사 증후군 및 심혈관계 질환 등의 발생에 중요한 역할을 한다. 이런 내장지방을 평가하는데 컴퓨터단층촬영이 좋은 방법으로 알려져 있으나, 고가이며, 방사선에 노출되며 그리고 쉽게 임상에서 이용하기 어렵다는 점에서 널리 사용되기에는 많은 제한점을 갖고 있다. 따라서 비교적 손쉽게 사용할 수 있는 비침습적인 평가 방법이 요구되고 있다. 본 연구에서는 내장비만을 평가하는데 복부 초음파의 유용성을 평가하기에 앞서, 컴퓨터단층촬영 Background : Visceral fat accumulation plays a major role in metabolic syndrome and increased cardiovascular risks. Clinical evaluation of visceral fat by CT is not widely used because of high cost, low availability, and ionizing radiation risk, and alter
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Retinoic acid와 dibutyryl cyclic AMP가 F9 embryonic carcinoma cell 분화 중 G1 Phase 관련 분자에 미치는 영향
박귀례(Kui Lea Park),김건홍(Kun Hong Kim),한순영(Soon Young Han),이유미(You Mie Lee),장성재(Seung Jea Jang) 대한약학회 1999 약학회지 Vol.43 No.3
Retinoic acid (RA) and dibutyryl cyclic AMP (dbcAMP) induce the differentiation of the multipotent embryonic carcinoma cell line, F9 cells, into parietal endoderm like cell. The F9 cells are highly proliferative doubling approximately 12 hours. S Phase is predominant, lasting 10 hours and G2/M phase occupies most of the remaining cycle (2 hours) and G1 phase is nearly non-existent. In this study, we showed the effect of RA and dbcAMP on the cell cycle associated molecules (especially around Gl phase) during F9 cell differentiation. Differentiation of F9 cells was induced by the combined addition of RA (10-7M) and dbcAMP (0.5mM), and cells were harvested daily up to 4 days. Flow cytometric analysis showed the prolongation of G1 phase around 30 hours after induction. Western blot analysis revealed that the amount of cyclin D1 and cdk2 were increased at day 4. However, histone H1 kinase activity of cdk2 was decreased. These data strongly suggest that RA and dbcAMP induce the growth arrest of F9 cells at G1 phase by decreasing the activity of cdk2, although they have increased the protein contents of cyclin Dl and cdk2. The reason for the discrepancy between the Hl kinase activity and protein contents are not clear yet.
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이유미,배수경,이옥희,이새원,김규원 한국생명과학회 1998 한국생명과학회 학술발표회 Vol.20 No.-
Angiogenesis, the formation of new blood vessels, is essential for the growth of tumor, and is also known to be tightly controlled by the various angiogenic factors and angiogenic inhibitors. As an in vivo method to quantitate angiogenesis, Matrigel with angiogenic factor was injected subcutaneously into C57BL/6 mice. As a result, Matrigel with bFGF markedly induced the many new blood vessels while no blood vessel was appeared in control mice. To investigate the role of hypoxia in balancing system of the angiogenic and antiangiogenic factors during hepatocarcinogeneis, the expression patterns of factors and inhibitors were examined using hepatocellular carcinoma cell lines, HepG2 and Hep3B. VEGF was highly induced by hypoxia in a time dependent manner. interestingly, IGF- II induced the expression of VEGF gene and synergistically increased that of VEGF when cotreated with hypoxia in HepG2 cells. In contrast, the expression of bFGF was not detected in HepG2 cells even in hypoxic condition by northern blot analysis. The tumor suppressor gene, p53 was decreased by the hypoxia in HepG2 cells. Taken together, VEGF and IGF- II are induced while p53 is down-regulated by hypoxia, suggesting that the expression changes of these factors coordinatively participate to form new blood vessels in early tumor mass.
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Culture of Rat Embryos During Early Organogenesis
Chin, Kang,Lee, You Mie,Rheu, Hang Mook 한국환경독성학회 1991 환경독성보건학회지 Vol.6 No.1
포유동물의 최기형성을 연구하는데 가장 많이 사용되는 동물인 설치류중 랫트의 배자를 이용하여, egg-cylinder기를 지나 head-fold가 이루어지는 초기체절기인 9.5일경부터 뇌, 눈, 귀, 심혈관계가 형성되는 시기인 11.5일까지 2일(48시간)동안, 체외에서 배양하였다. 그 배양의 최적조건은 실험실마다 다르기 때문에 최적조건확립을 목적으로 이 실험을 행하였다. 온도는 37℃?.2℃로 유지시키고 가스는 배양초기인 9.5일~10.5일 동안을 50% O₂, 5% CO₂, 90% N₂가스를 공급하여 10.5일부터 6시간 동안은 20% O₂,5% CO₂, 55% N₂가스를 배양계에 공급 했을 때 가장 좋은 결과가 나왔다. 배양배지로서는 랫트 IC혈청을 분리하여 열비동화시켜서 사용하였다. 이종혈청이나 합성배지를 첨가해서 배양할 경우는 랫트 혈청에 비해 성장이 좋지 않은 것으로 관찰되었다. 동물실험법의 대체법으로서 배자양법의 기본조건을 확립하여 최기형성 평가 방법으로서의 가능성을 제시하였다. Rat embryos were cultured out of the mother from the head-fold stages, 9.5 days to 11.5 days during which they start to develop the brain, eyes, ears and cardiovascular system etc. We principally did the basic experiment in order to establish the best condition of the rat whole embryo culture in our laboratory. The temperature in the culture system was maintained 37℃ ±0.2℃ for 48 hrs. The culture was carried out in humidified atmosphere of the air, intially, the bottles were equilibrated with 5% O₂, 5% CO₂, and 90% N₂ gas mixture. After 22 or 24 and 29 or 30 hrs the cultures were reequilibrated with 20% O₂, 5% C0₂, 75% N₂ and 40% 0₂, 5% CO₂, 55% N₂ respectively. Various types of sera were tested and for the purpose of minimizing the quantity of rat serum, artificial medium was also tried and it was determined that rat serum supported normal growth over a period of 48 hrs, based on total growth analysis and structural comparisons with in vivo specimens.
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