혐기처리 녹차의 처리조건에 따른 r-Aminobutyric Acid(GABA) 및 주요 성분의 변화

장지신(Ji-Shin Chang),이병순(Byong-Soon Lee),김영걸(Young-GUI Kim) 한국식품과학회 1992 한국식품과학회지 Vol.24 No.4

멧누에 ( Bombyx mandarina ) 난간단백질 유전자의 유전적 분석

성승현(Seung Hyun Sung),김순정(Soon Jeung Kim),염승식(Seung Shic Yum),김종길(Jong Kil Kim),김기세(Ki Sei Kim),김근종(Keun Chong Kim),문준옥(Jun Ok Moon),이병순(Byong Soon Lee),서동상(Dong Sang Suh) 한국유전학회 2001 Genes & Genomics Vol.23 No.4

Two multigene families, high-cysteine A(HcA) and high-cysteine B(HcB), are located within 140 kb region of chorion locus in Bombyx mori. Each family consists of 15 members and one of each members are transcribed in pairs. We report isolation and characterization of HcA and HcB genes of the wild type silkmoth, Bombyx mandarina. In this study, we examined 6 repeating units out of 15 HcA genes and 6 units of 15 HcB genes of B. mori and B. mandarina, respectively, and found that the arrangements and expression units of these are dramatically different. In addition, we found that most of the sequence variations were located within the C-variable region. These results suggest that the likely mechanism of concerted evolution is gene conversion-like event in HcA/HcB gene pairs.

한국 특산종 붉노랑상사화와 근연종의 rDNA 유전자의 염색체상의 위치 결정

이병순,김무열 한국유전학회 2000 Genes & Genomics Vol.22 No.1

The 18S-5.8S-26S ribosomal RNA gene (18S-26S rDNA) loci were examined directly on mitotic chromosomes in the Korean endemic Lycoris flavescens and its related species by fluorescent in situ hybridization (FISH) method. The prominent yellow signals of biotin labeled rDNA probes were detected on three chromosomes in Lycoris sanguinea var. koreana, six in L. flavescens, and eight in L. chinensis. All of their signals were located at the distal end of rod shaped chromosomes. The other weak signals were associated with centromeric regions of metacentric chromosomes. One or two nucleolus organizing regions (NORs) were also found in all three specis. This result is reported for the first time and offers new cytological markers in Lycoris.

전주 노랑초파리 자연집단내의 세포유전학적 연구

이병순 전주대학교 자연과학종합연구소 1996 전주대학교 자연과학연구소 학술논문집 Vol.9 No.2

The cytogenetic studies on inversion polymorphisms and gonadal dysgenesis(GD) were carried in September and October in 1995 from Chonju natural population of Drosophila melanogaster. On inversion polymorphisms, a total of 465 females, eight different inversions were found, all of them were identified to be paracentric inversion. An average frequency of wild flies carrying one or more inversions and mean number of inversions per individual were 42.3% and 0.512 respectively. To analysis of the gonadal dysgenesis with P-M system, a total of 201 lines were used. The P factor activity appeared to be nearly inactive. In tests for cytotype and P activity, about 30% of tested lines were characterized as M cytotype and the remaining lines(70%) as Q strains. Chonju wild populations of Drosophila melanogaster are composed of both Q and M strains.

한국 노랑초파리(Drosophila melanogaster) 집단의 염색체 역위의 생태지리적 변이

이병순 원광대학교 사범대학 과학교육연구소 1992 과학교육 연구지 Vol.1 No.1

From twenty two Korean localities a total of 4,857 Drosophila melanogaster were collected from late August to early October in 1985. Twenty different inversions were found, all of them were identified to be paracentric inversion. There were eight inversions on the second and twelve on the third chromosome. Two types of overlapping inversions were on the right arm of the third chromosome. As a whole of the twenty two populations, the average frequency of wild flies carrying one or more inversions and mean number of inversions per individual were 40.74% and 0.483. The frequency of four common cosmopolitan inversions showed a order of In(2L)t> In(2R)NS> In(3R)P> In(3L)P. Environmental variables such latitude, longitude, and several climatic factors were considered as to where any interrelationships between surrounding conditions and inversion behaviors exist. A correlation between latitude and In(2L)t, In(3L)P, and In(3R)C and between longitude and In(2L)t and In(2R)NS appeared significantly. It seems that the inversion frequencies correlate significantly with temperature and maximum wind speed in autumn in Korean populations of D. melanogaster.

초파리 自然集團內의 染色體變異에 關한 硏究

林洛龍,李秉順 全北大學校 敎育大學院 1986 敎育論叢 Vol.6 No.-

Inversion variations and those frequencies in a Jeonju natural population of Drosophila melanogaster were studied. From a total of 520 files tasted, 245 males and 275 females, 13 different inversions were found to be two major autosomal and paracentric only, except for X and 4th chromosomal as well as pericentric and the other chromosomal aberrations. Among the polymorphic inversions we found, four were common cosmopolitan ones, four rare cosmopolitans, three recurrent endemics and two unique endemics. And an overlapping inversion was observed and identified as overlapped with cosmopolitan In(3R) Mo and In(3R)P. The frequency of wild files carrying one or more inversions and the mean number of inversions per individual was 0.495 and 0.54 in the egg samples, and 0.374 and 0.51 in the male samples, respectively. However, the majority of inversion frequencies was led by common types of cosmopolitans. Some nonrandom associations of inversions appeared between chromosome 2 and 3, 2R vs. 3R(P〈0.01) in egg samples and 2L vs. 3R(P〈0.01) in male samples, but in an average showed randomness in both male and female tests. In male test, on the other hand, it seems that a significant linkage disequilibrium detected in the third chromosome was due probably to the coupling of In(3L)P and In(3R)P, with significant at 1% level. Although the shortages of homozygotes, with indices of deficiency homozygous both standard and inverted sequences, were estimated to be 3 to 6%, the statistical significances did not recognize from the Hardy-Weinberg principle.

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

초파리(D . melanogaster)집단내의 유전적 변이성

이태훈,이병순,임낙룡 한국유전학회 1988 Genes & Genomics Vol.10 No.3

Using a balancer stock of Drosophila melanogaster, known as A₁B_(18) which is translocated with second and third chromosomes and can blance the two major autosomes simultaneously, a total of four hundred seven sets of the two major autosomes, both second and third chromosome as a single unit, was examined. The male flies tested were sampled in October and November, 1980 at Chonju locating in southern part of Korea. The frequency of chromosomes carrying complete-lethal genes was 0.4889, 199 out of 407, and semi-lethals 0.2703, 110 out of 407. The average viability of all homozygotes including lethals was 0.1838 and 0.5133 excluding lethals. The average viabilities of heterozygotes did not differ significantly from each other. No difference in distribution of lethal genes appeared between the second and third chromosomes. Out of 145 lethal-carrying chromosomes tested, 49 were second chromosome lethals, 58 third lethals, 29 contained both second and third chromosome lethals, and 9 synthetic lethals which lethal gene is in fact located on neither second chromosome nor third chromosome. The distribution of lethals between second and third chromosomes did not show significant difference from each other. The average of effective sizes for the present population sampled was estimated to be approximately 5,000 pairs of flies. Although a linkage disequilibrium among inversions was detected only between the left and right arms of second chromosome, but not detected between the polymorphic inversions and lethal genes.

Chromosomal Iocalization of rDNA genes in Lycoris flavescens

Lee, Byong-Soon 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.3

The 18S, 5.8S, and 26S ribosomal RNA gene (rDNA) loci were detected directely on mitotic chromosomes of Lycoris flavescens using fluorescent in situ hybridization (FISH). The biotin labeled rDNA probe exhibits yellow fluorescent signals on six of the nineteen metaphase chromosomes of L flavesens that have been counterstained with propidium iodide. Of them four major signals were located at the distal end of telocentrics, and the other weak signals were associated with the nucleolus organizer region (NOR), respectively. This result is reported for the first time and offers new cytological markers in Lycoris.

Drosophila melanogaster 수개 집단내의 역위 다형현상

이태훈,이병순,임낙룡 한국유전학회 1986 Genes & Genomics Vol.8 No.1

Karyotypic variation studies in several wild populations of Drosophila melanogaster were made on the inversion polymorphisms. From four different localities, a total of 3154 females inseminated in nature were collected twice a year both in 1983 and in 1984 with a month apart, in September and October. In this study, twenty two different inversions were found: two on X-chromosomal, seven on the second, and thirteen on the third chromosome. Of those paracentric only, seven were either common or rare cosmopolitan, and the rest was either unique or recurrent endemic. The frequency of common cosmopolitan inversions was positively correlative with each other. The order of frequent inversions was changeable, but did not appear to repeat any cycle in seasonal or annual changes. The annual changes of inversion frequencies were found to display significant variations, and furthermore the total frequencies exhibited a trend to increase from year to year in all geographic populations in this study.