Enzymatic Preparation of Radiolabelled S-Adenosyl-L-$[methyl-^{14}C]$ methionine

임혜원,박인원,최명언,Rhim, Hye-Whon,Park, In-Won,Choi, Myung-Un 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.5

S-Adenosylmethionine (SAM)은 생체계에서 주요 메칠기 주게로 사용된다. 이 연구는 L-$[^{14}C-methyl]$methionine과 ATP 존재하에서 methionine adenosyltransferase (MAT)을 이용하여 방사성 표지된 SAM의 생합성 방법을 설명한다. MAT는 쥐 간으로부터 부분정제 하였으며 그 활동도의 최적조건을 재조절하였다. 생성률을 90% 이상이었고 비교적 높은 비방사성이 얻어졌다. S-Adenosylmethionine (SAM) serves as the major methyl group donor in biological system. This study describes an upgrade method for radiolabelled biosynthesis of SAM by methionine adenosyltransferase (MAT) in the presence of ATP and L-$[^{14}C-methyl]$methionine. The MAT was partially purified from rat liver and reoptimized the assay condition. The final yield of biosynthesis was better than 90% with relatively high specific radioactivity.

효소를 이용한 방사성 표지 s - Adenoxsyl - L - [ methyl - 14C ] methionine 의 생산

임혜원,박인원,최명언 ( Hye Whon Rhim,In Won Park,Myung Un Choi ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5

S-Adenosylmethionine (SAM) serves as the major methyl group donor in biological system. This study describes an upgrade method for radiolabelled biosynthesis of SAM by methionine adenosyltransferase (MAT) in the presence of ATP and L-[^(14)C-methyl]methionine. The MAT was partially purified from rat liver and reoptimized the assay condition. The final yield of biosynthesis was better than 90% with relatively high specific radioactivity.

비소세포 폐암에서 C-erbB-2와 VEGF 발현에 대한 면역조직화학적 연구

신종욱 ( Jong Wook Shin ),하경원 ( Kyung Won Ha ),최재철 ( Jae Cheol Choi ),김재열 ( Jae Yeol Kim ),박인원 ( In Whon Park ),최병휘 ( Byoung Whui Choi ),유재형 ( Jae Hyung Yoo ) 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.62 No.1

배경 및 목적: 인체암에서 C-erbB-2의 과발현에 대한 광범위한 연구가 시행되고 있지만, 임상적 의의에 대해서는 논란의 대상이 되고 있다. 악성종양에서 신생혈관형성은 암의 진행에 필수적인 요소이다. 본 연구에서는 비소세포 폐암에서 C-erbB-2의 과발현의 의미, 암조직의 신생혈관형성에 중요한 역할을 하는 VEGF의 발현 및 두 표지자의 연관성을 연구하였다. 방법: 파라핀 포매된 95예의 비소세포 폐암조직을 이용하여 avidin-biotin complex 법에 의한 면역조직화학적 염색을 시행하였으며, 연구재료는 원발성 편평세포암종 60예 그리고 원발성 선암종 35예를 대상으로 하였다. 결과: 비소세포 폐암에서 선암종의 C-erbB-2의 과발현은 편평세포암종보다 의미 있게 높게 나타났다. 임상병기에 따른 C-erbB-2와 VEGF의 과발현은 진행암에서 증가하는 경향을 나타내었으나, 초기암과의 유의한 차이는 없었다. 선암종에서 C-erbB-2와 VEGF의 발현은 유의하게 상관성을 나타내었다. 결론: 비소세포 폐암에서 C-erbB-2의 과발현은 암의 유형에 따라 다르다는 것을 알 수 있었으며, C-erbB-2의 과발현이 높게 나타나는 선암종에서는 이 성적과 암세포의 VEGF의 발현이 상관성을 나타낸다는 것을 알 수 있었다. Background: Mutated or deregulated expression of C-erbB-2 causes this gene to function as a potent oncogene. Vascular endothelial growth factor (VEGF) is a crucial angiogenic molecule in lung cancer. Both C-erbB-2 and VEGF can promote growth, proliferation and metastasis in non-small cell lung cancer (NSCLC). The purpose of this study was to investigate evaluate the relationship between the expressions of the C-erbB-2 and VEGF genes using immunohistochemistry. Materials and Methods: Ninety-five patients with NSCLC were involved (60 squamous cell carcinoma and 35 adenocarcinoma). The formalin-fixed paraffin embedded specimens were immunohistochemically stained for C-erbB-2 and VEGF using the avidin-biotin complex method. Results: Positive C-erbB-2 expression was observed more often in adenocarcinomas than squamous cell carcinomas (p<0.05). Although the immunohistochemical expressions of C-erbB-2 and VEGF in non-small-cell lung cancer showed increased tendencies at an advanced stage, the correlation between early and advanced cancers was insignificant. In adenocarcinomas, the expressions of VEGF and C-erbB-2 were significantly (p<0.05). Conclusion: The overexpression fo C-erbB-2 was significantly higher in adenocarcinomas than squamous cell carcinomas, and correlated with the expression of VEGF in adenocarcinomas of the lungs. (Tuberc Respir Dis 2007; 62: 43-50)

노인 환자에서 발생한 거짓막 괴사성 기관지 아스페르길루스증

이승은 ( Seung Eun Lee ),전은주 ( Eun Ju Jun ),송주한 ( Ju Han Song ),신종욱 ( Jong Wook Shin ),김재열 ( Jae Yeol Kim ),박인원 ( In Whon Park ),최병휘 ( Byoung Whui Choi ),최재철 ( Jae Chol Choi ),김미경 ( Mee Kyoung Kim ) 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.63 No.3

Invasive aspergillus tracheobronchitis is uncommon manifestation of infection due to Aspergillus species, occurring in severely immunocompromised patients who are generally neutropenic with haematological diseases, AIDS, or after heart and lung transplantation. The pseudomembranous form is the most severe condition and is usually fatal despite treatment with antifungal agents. However, there are a few cases reported with no apparent severe compromise in the host defences. We encountered a pseudomembranous necrotizing bronchial aspergillosis in a 73-year old male patient, who was treated successfully with antifungal agents. (Tuberc Respir Dis 2007; 63: 278-282)

국내 일개 대학병원에서 경험한 AIDS 환자의 폐 감염성 질환의 임상적 특성

정재우 ( Jae Woo Jung ),정진원 ( Jin Won Chung ),송주한 ( Ju Han Song ),전은주 ( Eun Ju Jeon ),이영우 ( Young Woo Lee ),최재철 ( Jae Cheol Choi ),신종욱 ( Jong Wook Shin ),박인원 ( In Whon Park ),최병휘 ( Byoung Whui Choi ),김재열 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.61 No.6

배경: AIDS환자에게서 기회감염은 가장 치명적인 합병증으로 대부분은 폐 감염의 형태로 발현된다. 본 연구는 타 국가에 비해 아직까지는 유병률이 낮아서 상대적으로 자료가 부족한 국내의 현황에서, HIV 감염자 및 AIDS 환자의 임상양상을 살펴보고 특히 AIDS 환자들이 주로 어떤 호흡기 질환으로 발현하며, 호흡기 질환이 발현된 AIDS 환자와 기타 폐외 질환으로 발현하거나, HIV 단순감염자와의 임상상에 어떤 차이가 있는지를 중점적으로 살펴보고자 하였다. 방법: 2001년부터 2006년 2월까지 중앙대학교 병원에 내원한 HIV 감염자 총28명을 대상으로, 이들 환자의 의무기록을 후향적으로 조사하여 분석하였다. 결과: 총 28명의 HIV 감염자 중 27명이 남자이었고 평균연령은 40.6세로 30대가 40%로 가장 많은 분포를 보였다. 이 중에서 폐질환을 동반한 경우가 11명이었고, 폐외질환 환자가 16명이었다. 폐질환을 동반환 환자군과 폐외질환자에서 내원시 CD4+림프구수와 HIV RNA 수는 평균 79.5/mm3, 48,903 IU/ml, 400/㎣, 60,256 IU/ml을 보여(P value: 0.001, 0.695) 폐감염이 동반된 경우에 더 심한 면역억제 상태가 심하였다. 폐감염의 종류로는 폐결핵, 주폐포자충 폐렴이 가장 많은 빈도를 차지하였으며, 이들의 평균 CD4+림프구 수를 보면, 폐결핵이 56/㎣, 주폐포자충폐렴이 42/㎣이었다. 결론: HIV에 관련된 폐질환이 동반된 경우 그렇지 않은 경우에 비해 월등히 면역억제가 심하였다. 이 중에서 폐결핵과 주폐포포자충 폐렴이 가장 흔한 형태의 폐감염이었다. 본 연구는 일개 대학병원에서 경험한 HIV 감염자와 특히 폐질환이 발현된 AIDS 환자의 임상상의 특징을 확인할 수 있었고 빠른 조기진단과 치료의 필요성을 다시 한번 확인하였다. Background: In AIDS patients, the respiratory tract is one of the most frequently involved site of by an opportunistic infection, and an even common, casual pulmonary infection manifests in a peculiar ways in AIDS patients. In Korea, because of the low prevalence of AIDS, there is insufficient data compared with other Southeast Asian counties. However, considering the sexual behavior of the younger generation, it is expected that it will not be long before AIDS becomes a major public health issue in Korea. This study examined the clinical manifestation of HIV positive people and AIDS patients. The pulmonary manifestion of HIV-positive was evaluated. This study focused on the differences in the clinical manifestation between AIDS patients with pulmonary disease and simple HIV positive people. The characteristics of common pulmonary infections in AIDS patients were also analyzed. Method: The medical records of 28 HIV positive patients who visited the hospital of ChungAng University Hospital from January, 2001 to February, 2006 were analyzed retrospectively. Result: Twenty-seven patients out of 28 HIV positive patients were male and the average age was 40.6(23-65). Patients in their thirties were most commonly affected. Elven patients had pulmonary diseases. Pulmonary tuberculosis (4 cases) and pneumocystis pneumonia (4 cases) were the most common respiratory infection. One patient showed a peculiar type of systemic cryptococcus. which was accompanied by lung and pleural dissemination. The CD4+lymphocyte count of patients with a pulmonary infection was significantly lower in patients with a pulmonary manifestation than those with only a HIV infection (79.5/㎣ vs 400/㎣, respectively)(p<.05). Patients with pulmonary disease were in a more severe immunosuppressive state. There were 4 patients with pulmonary tuberculosis, 4 with pneumocystis pneumonia, 4 with secondary syphilis, 2 with primary syphilis, and 2 with HZV infection. The average CD4 lymphocyte counts was 56/㎣ in those with pulmonary tuberculosis, 42/㎣ in those with pneumocystis pneumonia, and 455/㎣ in those with secondary syphilis. Conclusion: This study examined the clinical manifestation of HIV positive patients, particularly AIDS patients with pulmonary disease, A more severe immunosuppressive status was observed in HIV-related pulmonary compared with those with HIV-related extrapulmonary disease, and the frequency of pulmonary tuberculosis in pulmonary disease was higher than expected. Respiratory infection in AIDS manifest in uncommon ways e.g. disseminated cryptococcosis involving the lung and pleura. Evidently, AIDS patients with a respiratory infection have a more severe form of immunosuppression than those with a simple HIV infection. As expected, patients with a pulmonary infection were in a more severe immunosuppressed state than those with a simple HIV infection. Opportunistic infections can show peculiar clinical presentations in AIDS patients. (Tuberc Respir Dis 2006; 61: 554-561)