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감자 "추백"에 발생한 Tobacco mosaic virus의 특성
김정수 ( Joung Soo Kim ),김재현 ( Jae Hyun Kim ),최국선 ( Gug Seoun Choi ),채수영 ( Soo Young Choi ),김현란 ( Hyun Ran Kim ),정봉남 ( Bong Nam Joung ),최용문 ( Yong Mun Choi ) 한국식물병리학회 2003 식물병연구 Vol.9 No.2
An isolate of Tobacco mosaic virus (TMV) was isolated from potato cultivar ``Chubak`` showing vein clearing and mild mosaic at Namhae in Korea. This isolate, TMV-St, was differentiated from other tobamoviruses based on biological properties, serological relationships and nucleotide sequence analyses of coat protein genes. TMV-St caused typical symptoms on four indicator plants as compared to the tobamovirus of TMVU1, Pepper mild mottle virus (PMMoV), and Tomato mosaic virus (ToMV), which caused economic losses in Solanaceous vegetables, tomato, pepper, and eggplant. Remarkably, the TMV-St induced distinctly different symptom of systemic chlorotic spots on Chenophodium murale. On C. murale, Gomphorena globosa, and Nicotiana rustica, the four viruses were classed by the virulence of systemic or local infections. In serological test TMV-St antiserum showed a precipitation line with each other tabamovirus. The CP gene of TMV-St contain 477 nucleotides, and the nucleotides sequence was the most similar to that of TMV-U1.
Educational Lecture 1-2 (EL 1-2) : Phototesting
김정수 ( Joung Soo Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1
Phototesting is specialized tests which confirm the presence of an abnormal cutaneous reaction to light. It is also used to establish a diagnosis in patients with suspected photodermatoses and to determine threshold dose and wavelength dependence. For example, phototesting is used to confirm a diagnosis of solar urticaria, systemic phototoxicity, or photosensitivity in certain idiopathic photodermatoses, such as chronic actinic dermatitis. It can also help to characterize certain secondary photodermatoses induced by endogenous agents, such as erythropoietic protoporphyria or photosensitive forms of lupus erythematosus. The phototest procedure comprises three steps. First, the threshold dose for erythema response is determined by minimal erythema dose (MED) testing. To determine the MED, phototesting is conducted on the back, buttocks or inner aspect of the forearm. Small skin fields are irradiated with increasing ultraviolet (UV) doses. Step two is reproduction of photodermatosis by a provocation phototest procedure. Repeated irradiations are performed with polychromatic UVA and/or UVB on consecutive days to provoke the photodermatoses. Third is photopatch testing to identify photoallergy.It is patch testing with the addition of UV radiation to induce formation of the photoallergen. It is used to diagnose photoallergy and photocontact dermatitis. In advance of phototesting, the patients need to be informed of the risk of sensitization as recognized in routine patch testing and be aware of the possibility of strong provocation test results. The test should be performed on a part of the body that is generally not exposed to sunlight. Photopatch testing should not be performed when the dermatitis is active. In this lecture, we will discuss about the procedures and applications of the phototesting, focusing on the clinical usefulness.
김정수 ( Joung Soo Kim ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1
Phototesting is specialized test which confirms the presence of an abnormal cutaneous reaction to light. It is also used to establish a diagnosis in patients with suspected photodermatoses such as polymorphic light eruption, actinic prurigo, hydroa vacciniforme, chronic actinic dermatitis and solar urticarial, and to determine threshold dose and wavelength dependence. The phototest procedure is composed of three steps. 1. Phototest: Determination of MED (Minimal erythema dose) - Confirm the presence of a photosensitivity disorder - Uninvolved skin of the patient`s back, abdomen or inner forearms is exposed to different doses of UVB (Ultraviolet B), UVA (Ultraviolet A) and visible monochromatic or broad-spectrum radiation. - The assessment of cutaneous response consists of immediate observation of possible urticarial lesions (for solar urticaria) and the MED reading performed 24hr later. - Most helpful for the diagnosis of immunologically mediated photodermatoses 2. Photoprovocation: Reproduction of photodermatosis by a provocation phototest procedure - Used to reproduce an eruption so that the morphology of the lesion can be examined in greater detail - Usually, 80% of the MED is used as the starting dose, and an increase of 10% to 20% is performed on consecutive days. - The test field should be larger than that used in phototesting, as photoprovocation sometimes induces separate papules that could be underestimated. 3. Photopatch testing: Photopatch testing is conducted to identify photoallergy - Used to evaluate patients with photoallergic contact dermatitis. - Two sets of photoallergen panels are placed on uninvolved sites of the skin, usually on the upper back. - One set is removed after 24hr and irradiated with UVA of 5-10 J/cm2. After 48 and 72hr, both sets of patch tests (the irradiated and nonirradiated sides) are evaluated for a positive reaction. Precautions - need to be informed of the risk of sensitization - should be performed on a part of the body that is generally not exposed to sunlight - should not be performed when the dermatitis is active.
김정수 ( Joung Soo Kim ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2
Phototesting is specialized test which confirms the presence of an abnormal cutaneous reaction to light. It is also used to establish a diagnosis in patients with suspected photodermatoses such as polymorphic light eruption, actinic prurigo, hydroa vacciniforme, chronic actinic dermatitis and solar urticarial, and to determine threshold dose and wavelength dependence.