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돼지호흡기코로나바이러스 감염증의 감별진단과 항체분포 조사
김은경 ( Eun Gyeong Kim ),손병국 ( Byeong Kuk Son ),이종민 ( Jong Min Lee ),김도경 ( Tho Kyoung Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4
Porcine respiratory coronavirus (PRCV) is antigenically related to transmissible gastroenteritis virus (TGEV). Differential serological diagnosis between PRCV and TGEV infection is not possible with the classical sero-neutralization test. Infection with PRCV or TGEV induces antibodies which neutralize both viruses to the same titer. However, the enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (PCR) can differentiate between PRCV and TGEV infection. This study was carried out to investigate the prevalence of PRCV infection of swine in Gyeongnam province. A total of 391 serum samples from 37 herds in Gyeongnam were examined for antibody to PRCV using blocking ELISA. All serum samples were collected from 130- to 150-day-old pigs between August and December 2006. By ELISA, 182 out of 391 sera tested (46.5%) and 29 out of 37 sample herds (78.4%) were positive against PRCV. Our data suggested that seropositive herds for PRCV are distributed diffusely throughout Gyeongnam. The PCR methods were established to diagnose PRCV spike protein (S) gene. PCR were conducted to identify the PRCV genome against 150 pigs in PRCV antibody positive herds.
Real-time PCR을 이용한 돼지써코바이러스 감염증 진단법 연구
김은경 ( Eun Gyeong Kim ),황보원 ( Bo Won Hwang ),이종민 ( Jong Min Lee ),손병국 ( Byeong Guk Son ),박호정 ( Ho Jung Park ),김도경 ( Tho Kyoung Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4
Assay for the detection and quantification of porcine circovirus type 2 (PCV 2) with the Real-time PCR were developed. TaqMan probe real-time using a set of primer/probe was developed for detection of PCV 2. In this study we applied Real-time PCR assay to 320 samples, collected from pig farms. In 151 of 320 samples, PCV 2 DNA was detected by conventional PCR assay. All samples positive for PCV 2 DNA in conventional PCR assay were also positive in Real-time PCR assay, but 69 of 169 samples that tested negative for PCV 2 DNA in conventional assay were tested positive in TaqMan probe real-time PCR assay. The test of TaqMan probe real-time PCR resulted in detection and quantification limits of 101 copies per sample. TaqMan probe real-time PCR assay increased the number of samples in which PCV 2 was detected by 21%. TaqMan probe real-time PCR assay is very efficient method in contrast to the conventinal PCR, becoming increasingly important method for gene analysis.