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Molecular marker evaluated for heat tolerance in wheat
Jae-Han Son,Kyeung-Hoon Kim,Chon-Sik Kang,Young-Keun Cheong,Jong-Chul Park,Kyong-Ho Kim,Yang-Kil Kim,Young-Jin Oh,Jong-Ho Park,Tae-Hwa Song,Jae-Seong Choi,Bo-Kyeong Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
High temperature is one of major environmental stress. Some of molecular markers related heat stress or tolerance have been reported by many researchers. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR ect. was tried to selection of useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. These markers were linked to important traits including grain filling duration, HIS (Heat susceptibility index) grain filling duration. In this study, we tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey and six Korean wheat cultivars using 14 SSR markers. The average of the number of alleles and PIC values in this study were 6.14 and 0.63, respectively. Two major clades and six sub clades were grouped by phylogenetic tree using UPGMA program. Six Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance.
Elimination of Lead by TTFD and TPD from Central Nervous System of Postnatally Lead - exposed Rats
Cheong, Jae Hoon,Ahn, Jae Suk,Seo, Dong Ook,Kim, Kyeong Man,Ko, Kwang Ho 한국환경독성학회 1992 환경독성보건학회지 Vol.7 No.1
Amount of lead burden in a tissue reflects poisoning of lead in that tissue, so is the removal of lead directly connected to curement of lead poisoning. The purpose of present study was to investigate the relative effects of penicillamine and thiamine tetrahydrofurfuryl disulfide (TTFD) or thiamine propyl disulfide (TPD) in the removal of lead from rat brain tissue treated with excessive lead. Wistar rat pups of both sexes were used in this experiment. Within 1 day of parturition, experimental mothers nursing their pups as well as rat pups were given drinking water containing 0.2% lead acetate, TTFD 20 mg/1.2 L (2 mg/kg/day), TPD 20 mg/1.2 L (2 mg/kg/day), penicillamine 40 mg/1.2 L (40 mg/kg/day), 0.2% lead acetate+TTFD 20 mg/1.2 L (2 mg/kg/day), 0.2% lead acetate+TPD 20 mg/1.2 L (2 mg/kg/day) or 0.2% lead acetate+penicillamine 40 mg/1.2 L (40 mg/kg/day) ad libitum, throughout the entire period of experiment. Rat pups in the control group received normal tap water. The animals were sacrificed by decapitation on the day when they become 2 or 8 weeks of age. Brains were dissected into five regions: telencephalon, diencephalon, midbrain, pons/medulla and cerebellum. The dissected brain tissues were lyophillized and then solubilized by acid mixture (nitric acid+sulfuric acid). Lead levels in the solubilized brain tissues were measured by the inductively coupled plasma. In lead-exposed rats, lead levels were significantly higher than those of control group in all brain regions, lead levels in brain regions of TTFD or TPD group were generally lower than those of control group. The simultaneous administration of lead with TTFD or TPD to animals caused significant decrement of lead from all brain regions. In the elimination of lead from brain regions, effectiveness of TTFD or TPD was equivalant to penicillamine.