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( Cong Wang ),( Yul-lye Hwang ),( Xue Mei Li ),( Soo Jung Kim ),( Ming Ji Zhu ),( Jeung-hoon Lee ),( Ri-hua Jiang ),( Chang Deok Kim ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.3
Background: Sebocytes are the major cells of sebaceous gland. The essential role of sebocytes is the production of sebum, a specific lipid mixture, that covers the body surface and provides the barrier function. At puberty, sebum production increases under the effects of various stimuli including androgens and insulin-like growth factor-1 (IGF-1). Excessive sebum production changes the microenvironment surrounding hair follicle, often leading to the onset of acne. Objective: We previously performed screening test using cultured human sebocytes, and found that bilobetin had a potential for inhibiting lipid production. The aim of this study is to demonstrate the effects of bilobetin on IGF-1-induced lipogenesis in sebocytes. Methods: We pretreated simian virus 40 T (SV40T)-transformed sebocytes with bilobetin then stimulated with IGF-1. Effects of bilobetin on lipogenesis of sebocytes were examined by thin layer chromatography and Western blot. Results: Bilobetin markedly inhibited IGF-1- induced lipid production in sebocytes, especially in terms of production of squalene and wax ester. Supporting these results, bilobetin showed significant inhibitory effect on squalene synthase promoter activity. In addition, bilobetin significantly down-regulated lipogenic transcription factors such as sterol response element binding protein (SREBP)-1 and SREBP-2. To delineate the possible action mechanism, we investigated the effect of bilobetin on intracellular signaling. As a result, bilobetin inhibited IGF-1-induced phosphorylation of AKT. Conclusion: Together, these results suggest that bilobetin has an inhibitory potential on sebum production in sebocytes, being applicable for acne treatment. (Ann Dermatol 31(3) 294∼299, 2019)
( Soo-jung Kim ),( Yul-lye Hwang ),( Su-hyuk Yim ),( Dongkyun Hong ),( Chong Won Choi ),( Kyung Eun Jung ),( Young-joon Seo ),( Chang-deok Kim ),( Jung Min Shin ),( Young Lee ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2
Background: Female-pattern hair loss (FPHL) is a common hair loss disorder in women. The various treatments include topical minoxidil and 17α-estradiol, and oral anti-androgens. However, the clinical efficacy of 5α-reductase inhibitors remains controversial. Objectives: We evaluated the clinical utility of dutasteride in FPHL patients, and how dutasteride promotes hair growth. Methods: We evaluated hair follicle density and thickness before and after oral dutasteride treatment in 24 FPHL patients. We measured β-catenin activity in primary cultures of human dermal papilla cells (DPCs) using the TOP Flash reporter assay and Western blotting. The expression levels of genes promoting hair growth were quantitatively assessed via Q-PCR. Results: The mean vertex hair density increased significantly from 67±14 to 76±13/ ㎠ (P=0.001) and the mean occipital hair density increased from 89±11 to 94±13/cm2 (P=0.012) after dutasteride treatment. When DPCs were treated with dutasteride, TOP Flash activity was increased in a dose-dependent manner and the protein level of non-phosphorylated (active) β-catenin was also increased by dutasteride. The mRNA level of vascular endothelial growth factor was increased, but the mRNA levels of keratinocyte growth factor, IGF-1,and Noggin were not affected by dutasteride. Conclusion: This study shows a novel mechanism of dutasteride in promoting hair growth and provides support for possible clinical application of 5α-reductase inhibitors for treatment of FPHL.
( Dong-kyun Hong ),( Mi-ra Choi ),( Yul-lye Hwang ),( Jae Kyung Lee ),( Young Lee ),( Young-joon Seo ),( Sooil Kim ),( Young-ho Lee ),( Chang-deok Kim ),( Jeung-hoon Lee ) 대한피부과학회 2021 Annals of Dermatology Vol.33 No.4
Background: Psoriasis is a chronic inflammatory skin disease. The etiology of psoriasis is not fully understood, but the genetic background is considered to be the most important factor. To date, many psoriasis-related genes have been discovered, but the role of many important genes has not been well understood. Objective: The purpose of this study is to uncover possible roles of MDA5 in psoriasis. Methods: Expression of MDA5 was investigated using immunohistochemistry. Then, MDA5 was overexpressed in keratinocytes using a recombinant adenovirus. Results: As a result of immunohistochemical staining, the expression of MDA5 was significantly increased in the epidermis of psoriasis compared to normal skin. Similarly, the expression of MDA5 was increased in imiquimod-induced psoriasiform dermatitis model. In cultured keratinocytes, toll-like receptor 3 agonist poly(I:C) induced expression of MDA5 at both mRNA and protein levels. When MDA5 was overexpressed using a recombinant adenovirus, poly(I:C)-induced cytokine expression was significantly increased. Finally, MDA5 overexpression significantly inhibited calcium-induced differentiation of keratinocytes. Conclusion: These results suggest that MDA5 increases in psoriasis and negatively regulates keratinocyte differentiation. (Ann Dermatol 33(4) 339∼344, 2021)
Cedrol Enhances Extracellular Matrix Production in Dermal Fibroblasts in a MAPK-Dependent Manner
( Mu Hyun Jin ),( Sun Gyoo Park ),( Yul Lye Hwang ),( Min Ho Lee ),( Nam Ji Jeong ),( Seok Seon Roh ),( Young Lee ),( Chang Deok Kim ),( Jeung Hoon Lee ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.1
Background: The extracellular matrix (ECM) produced by dermal fibroblasts supports skin structure, and degradation and/or reduced production of ECM are the main causes of wrinkle formation. Objective: The aim of this study was to identify the active ingredient that enhances ECM production in dermal fibroblasts. Methods: Polarity-based fractionation was used to isolate the active ingredient from natural extracts, and the effects of cedrol (isolated from Pterocarpus indicusirginia) on ECM production in cultured human dermal fibroblasts was investigated by reverse transcription- polymerase chain reaction, enzyme linked immunosorbent assay, and Western blot analysis. Results: Cedrol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was markedly increased by cedrol, indicating that enhanced ECM production is linked to activation of intracellular signaling cascades. Conclusion: These results indicate that cedrol stimulates ECM production, with possible applications to the maintenance of skin texture. (Ann Dermatol 24(1) 16∼21, 2012)
Stimulation of the Extracellular Matrix Production in Dermal Fibroblasts by Velvet Antler Extract
( Seok Seon Roh ),( Min Ho Lee ),( Yul Lye Hwang ),( Hyun Hee Song ),( Mu Hyun Jin ),( Sun Gyoo Park ),( Cheon Koo Lee ),( Chang Deok Kim ),( Tae Jin Yoon ),( Jeung Hoon Lee ) 대한피부과학회 2010 Annals of Dermatology Vol.22 No.2
Background: Fibroblasts produce many components of the extracellular matrix (ECM) and so they contribute to the maintenance of connective tissue integrity. Objective: The aim of this study is to evaluate the effect of velvet antler extract (VAE) on the ECM production of dermal fibroblasts cultured in vitro. Methods: Primary cultured human dermal fibroblasts were treated with VAE, and then the ECM production was determined by RT-PCR, ELISA and Western blot analysis. Furthermore, the change of gene expression according to VAE treatment was evaluated by cDNA microarray. Results: VAE accelerated the growth of fibroblasts in a dose-dependent manner. VAE increased the production of several ECM components, including type 1 collagen, fibronectin and elastin. In line with these results, the phosphorylations of p42/44 ERK and p38 mitogen-activated protein kinase were markedly increased by VAE, suggesting that the enhancement of ECM production may be linked to the activation of intracellular signaling cascades. VAE also significantly increased cell migration on an in vitro scratch wound test. In cDNA microarray, many genes related with connective tissue integrity were identified to be up-regulated by VAE. Conclusion: These results suggest that VAE has a potential to stimulate ECM production, and VAE may be applicable for maintaining the skin`s texture. (Ann Dermatol 22(2) 173~179, 2010)
여성형탈모에서 두타스테리드의 임상적 치료 효과 및 작용 기전에 대한 연구
김수정 ( Soo Jung Kim ),황율례 ( Yul-lye Hwang ),임수혁 ( Su-hyuk Yim ),홍동균 ( Dong Kyun Hong ),최종원 ( Chong Won Choi ),정경은 ( Kyung Eun Jung ),서영준 ( Young-joon Seo ),김창덕 ( Chang-deok Kim ),신정민 ( Jung Min Shin ) 대한피부과학회 2020 대한피부과학회지 Vol.58 No.4
Background: Female-pattern hair loss (FPHL) is a common hair loss disorder in women. The various treatments include topical minoxidil and 17α-estradiol, as well as oral anti-androgens. However, the clinical efficacy of 5α -reductase inhibitors remains controversial. Objective: We evaluated the clinical utility of dutasteride in FPHL patients and how dutasteride promotes hair growth. Methods: We evaluated hair follicle density and thickness before and after oral dutasteride treatment in 24 patients with FPHL. We measured β-catenin activity in primary cultures of human dermal papilla cells (DPCs) using the TOP Flash reporter assay and Western blotting. The expression levels of genes promoting hair growth were quantitatively assessed using quantitative polymerase chain reaction (Q-PCR). Results: The mean vertex hair density increased significantly from 67±14 to 76±13/cm<sup>2</sup> (p=0.001) and the mean occipital hair density increased from 89±11 to 94±13/cm<sup>2</sup> (p=0.012) after dutasteride treatment. However, the mean hair thickness did not increase. When DPCs were treated with dutasteride, TOP Flash activity increased in a dose-dependent manner, and the protein level of non-phosphorylated (active) β-catenin also increased. The mRNA level of vascular endothelial growth factor increased, but the mRNA levels of the keratinocyte growth factor, insulin growth factor-1, and Noggin were not affected by dutasteride. Conclusion: This study shows a novel mechanism of dutasteride in promoting hair growth and provides support for the possible clinical application of 5α-reductase inhibitors for the treatment of FPHL. (Korean J Dermatol 2020;58(4):231∼238)