초등학교 고학년 학생의 장애아동에 대한 인식 조사

김보람,김호영,손유라,오진주,윤슬기,이효정,장문영 대한감각통합치료학회 2011 대한감각통합치료학회지 Vol.9 No.1

목적 : 초등학교 고학년 학생의 장애아동에 대한 인식을 알아보는 것이다. 연구방법 : 연구 대상은 김해시에 소재한 통합교육을 실시하지 않는 초등학교의 4, 5, 6학년의 비장애 아동이었다. 연구 기간은 2008년 10월 2일부터 10월 17일까지였으며, 설문지를 사용하였다. 설문지의 하위 영역은 사귐, 활 동, 학업 영역의 세 부분으로 각 10문항으로 이루어져 있으며 SPSS 12.0을 사용하여 분석하였다. 결과 : 장애아동에 대한 인식조사 결과 첫째, 성별에 따른 인식은 남학생보다 여학생의 평균 점수가 높았으나 통계 적으로 유의미한 차이는 없었다. 둘째, 학년에 따른 인식은 4학년의 평균 점수가 가장 높았고 6학년이 가장 낮았으 며 통계적으로 유의미한 차이가 있었다(F=3.546, p<0.05). 셋째, 장애인 접촉 유무에 따른 인식은 접촉 경험이 없 는 학생이 있는 학생보다 평균점수가 높았으나 통계적으로 유의미한 차이는 없었다. 결론 : 비장애학생의 장애아동에 대한 인식은 학년에 따라 차이가 있었다. 본 연구는 작업치료사들에게 장애아동에 대한 또래집단의 인식에 대한 정보를 제공함으로써 학교환경에 대한 이해를 도울 수 있을 것이다. Objective : The purpose of this study was to investigate attitude of grade-schoolers toward children with disabilities. Methods : The subjects of this study were grade-schoolers who have not received an inclusive education in Gim-hae. The study was surveyed from October second to 17th in 2008. The survey was categorized into three parts; comradeship, activity, and study. Each part of the survey consists of 10 questions. Data were analyzed by SPSS (version 12.0). Results : The results were as follows; First, the attitude of girls toward disabled students was non-significant compared to boys. Second, the order of attitude degree in grade of students was 4, 6, and 5. The attitude toward children with disabilities showed statistically significant difference according to grade(F=3.546, p<0.05). Third, the attitude of students who have experience of contact with people with disabilities was non-significant compared to non-experienced students. Conclusion : In conclusion, therefore peer groups in lower class levels showed more positive attitude toward the children with disabilities. The result will be useful information for understanding disabled children and making positive attitude. Also it is expected that occupational therapists help disabled children to participate in school life successfully using these information.

Height and Cognitive Function in an Older Korean Population

Jeong, Seul-Ki,Seo, Man-Wook,Kim, Young-Hyun 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.2

Background: Some kind of adult anthropometry was reported as a marker of early life environment, and could be associated with cognitive impairment or dementia. This study aimed to examine whether height was associated with various cognitive domains. Method: A community study of 235 individuals aged 65 and over was performed in Noam-dong, Namwon city, Jeonbuk province. Cognitive function was ascertained from the Korean version of modified Mini-Mental State Examination (K-mMMSE), and cognitive domains like memory, visuoconstruction, and word fluency were derived from the previous K-mMMSE scores. Anthropometric measurement included height. Results: Height showed significant correlations with memory (r=0.41), orientation (r=0.49), word fluency (r=0.40), and visuoconstruction (r=0.48, all p values ;0.001). Among the domains, taller stature was significantly associated with higher scores of orientation and visuoconstruction, independently of age, sex, education, and the other con-founders. Conclusion: Taller height was associated with better cognitive performance independently of educational attainment. Height could be used as a marker of cognitive reserve capacity in our elderly population.

Data management and functional annotation of the Korean reference plasma proteome

Jeong, Seul-Ki,Lee, Eun-Young,Cho, Jin-Young,Lee, Hyoung-Joo,Jeong, An-Sung,Cho, Sang Yun,Paik, Young-Ki WILEY-VCH Verlag 2010 Proteomics Vol.10 No.6

<P>As human plasma is clinically valuable, reference data from healthy donors can be a useful source for serological biomarker studies. To make a reliable protein catalog of the Korean plasma proteome, various experimental methods, such as 1-D HPLC, 2-D LC, and narrow ranged 2-DE prior to MALDI-TOF and LC-MS/MS, were used to identify unique plasma proteins in this population. To compile candidates with high confidence, two different search engines were used to select proteins with a false discovery rate of less than or equal to 1%. From this rigorous selection process, we initially identified 494 distinct Korean plasma proteins. After multilevel stepwise filtrations with stringent, identification parameters were applied to acquire plasma protein list with the maximum confidence; a total 185 distinct plasma proteins were identified and integrated into our Korean human plasma proteome project database along with several bioinformatics analysis results, including gene ontology, biological pathways, tissue expression, and disease association. This is the first publicly available single ethnic group-specific plasma proteome database (http://proteomix.org/khppp/).</P>

Poster Session 1 : An integrated functional protein database of Caenorhabditis elegans

( Seul Ki Jeong ),( Min Seok Kwon ),( Young Ki Paik ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

GenomewidePDB, a Proteomic Database Exploring the Comprehensive Protein Parts List and Transcriptome Landscape in Human Chromosomes

Jeong, Seul-Ki,Lee, Hyoung-Joo,Na, Keun,Cho, Jin-Young,Lee, Min Jung,Kwon, Ja-Young,Kim, Hoguen,Park, Young-Mok,Yoo, Jong Shin,Hancock, William S.,Paik, Young-Ki American Chemical Society 2013 Journal of proteome research Vol.12 No.1

<P>In an effort to map the human proteome, the Chromosome-centric Human Proteome Project (C-HPP) was recently initiated. As a member of the international consortium working on this project, our laboratory developed a gene-centric proteomic database called GenomewidePDB, which integrates proteomic data for proteins encoded by chromosomes with transcriptomic data and other information from public databases. As an example case, we chose chromosome 13, which is the largest acrocentric human chromosome with the lowest gene density and contains 326 predicted proteins. All proteins stored in GenomewidePDB are linked to other resources, including neXtProt and Ensembl for protein and gene information, respectively. The Global Proteome Machine database (GPMdb) and the PeptideAtlas are also accessed for observed mass spectrometry (MS) information, while Human Protein Atlas is used for information regarding antibody availability and tissue expression, respectively. Gene ontology disease information is also included. As a pilot work, we constructed this GenomewidePDB with the identified 3615 proteins including 53 chromosome 13-origin proteins that are present in normal human placenta tissue. Thus, developing a comprehensive database containing actual experimental proteomics data will provide a valuable resource for cross chromosomal comparison in the C-HPP community.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2013/jprobs.2013.12.issue-1/pr3009447/production/images/medium/pr-2012-009447_0004.gif'></P>

PanelComposer: a web-based panel construction tool for multivariate analysis of disease biomarker candidates.

Jeong, Seul-Ki,Na, Keun,Kim, Kwang-Youl,Kim, Hoguen,Paik, Young-Ki American Chemical Society 2012 JOURNAL OF PROTEOME RESEARCH Vol.11 No.12

<P>Measuring and evaluating diagnostic efficiency is important in biomarker discovery and validation. The receiver operating characteristic (ROC) curve is a graphical plot for assessing the performance of a classifier or predictor that can be used to test the sensitivity and specificity of diagnostic biomarkers. In this study, we describe PanelComposer, a Web-based software tool that uses statistical results from proteomic expression data and validates biomarker candidates based on ROC curves and the area under the ROC curve (AUC) values using a logistic regression model and provides an ordered list that includes ROC graphs and AUC values for proteins (individually or in combination). This tool allows users to easily compare and assess the effectiveness and diagnostic efficiency of single or multiprotein biomarker candidates. PanelComposer is available publicly at http://panelcomposer.proteomix.org/ and is compatible with major Web browsers.</P>

GenomewidePDB 2.0: A Newly Upgraded Versatile Proteogenomic Database for the Chromosome-Centric Human Proteome Project

Jeong, Seul-Ki,Hancock, William S.,Paik, Young-Ki American Chemical Society 2015 Journal of proteome research Vol.14 No.9

<P>Since the launch of the Chromosome-centric Human Proteome Project (C-HPP) in 2012, the number of “missing” proteins has fallen to 2932, down from ∼5932 since the number was first counted in 2011. We compared the characteristics of missing proteins with those of already annotated proteins with respect to transcriptional expression pattern and the time periods in which newly identified proteins were annotated. We learned that missing proteins commonly exhibit lower levels of transcriptional expression and less tissue-specific expression compared with already annotated proteins. This makes it more difficult to identify missing proteins as time goes on. One of the C-HPP goals is to identify alternative spliced product of proteins (ASPs), which are usually difficult to find by shot-gun proteomic methods due to their sequence similarities with the representative proteins. To resolve this problem, it may be necessary to use a targeted proteomics approach (e.g., selected and multiple reaction monitoring [S/MRM] assays) and an innovative bioinformatics platform that enables the selection of target peptides for rarely expressed missing proteins or ASPs. Given that the success of efforts to identify missing proteins may rely on more informative public databases, it was necessary to upgrade the available integrative databases. To this end, we attempted to improve the features and utility of GenomewidePDB by integrating transcriptomic information (e.g., alternatively spliced transcripts), annotated peptide information, and an advanced search interface that can find proteins of interest when applying a targeted proteomics strategy. This upgraded version of the database, GenomewidePDB 2.0, may not only expedite identification of the remaining missing proteins but also enhance the exchange of information among the proteome community. GenomewidePDB 2.0 is available publicly at <uri xlink:href='http://genomewidepdb.proteomix.org/' xlink:type='simple'>http://genomewidepdb.proteomix.org/</uri>.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2015/jprobs.2015.14.issue-9/acs.jproteome.5b00541/production/images/medium/pr-2015-00541m_0009.gif'></P>

Poster Session : Face: functional annotation of preteime in C. elegans

( Seul Ki Jeong ),( Min Seok Kwon ),( Young Ki Paik ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-

ASV-ID, a Proteogenomic Workflow To Predict Candidate Protein Isoforms on the Basis of Transcript Evidence

Jeong, Seul-Ki,Kim, Chae-Yeon,Paik, Young-Ki American Chemical Society 2018 Journal of proteome research Vol.17 No.12

<P>One of the goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to map and characterize the functions of protein isoforms produced by alternative splicing of genes. However, identifying alternative splice variants (ASVs) via mass spectrometry remains a major challenge, because ASVs usually contain highly homologous peptide sequences. A routine protein sequence analysis suggests that more than half of the investigated proteins do not generate two or more uniquely mapping peptides that would enable their isoforms to be distinguished. Here, we develop a new proteogenomics method, named “ASV-ID” (alternative splicing variants identification), which enables identification of ASVs by using a cell type-specific protein sequence database that is supported by RNA-Seq data. Using this workflow, we identify 1935 distinct proteins under highly stringent conditions. In fact, transcript evidence on these 841 proteins helps us distinguish them from other isoforms, despite the fact that these proteins are not predicted to make 2 or more uniquely mapping peptides. We also demonstrate that ASV-ID enables detection of 19 differently expressed isoforms present in several cell lines. Thus, a new workflow using ASV-ID has the potential to map yet-to-be-identified difficult protein isoforms in a simple and robust way.</P> [FIG OMISSION]</BR>

Poster Session : Biotechnology ; Proteome analyst: a comprehensive human plasma proteome database with versatile analysis functions

( Seul Ki Jeong ),( Min Seok Kwon ),( Eun Sik Kim ),( Eun Young Lee ),( Sang Yun Cho ),( Young Ki Paik ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-