A New 5α,8α-Epidioxy Sterol from a Marine Sponge Psammocinia Species

Hye Jin Park1,,Xuan Luo1,,Jongki Hong2,,Dong-kyoo Kim3,,Kwang Sik Im1, and,Jee H. Jung1* 한국생약학회 2005 Natural Product Sciences Vol.11 No.4

An investigation of the MeOH soluble fractions of a marine sponge Psammocinia sp. (Order:Dictyoceratida) led to the isolation of a new epidioxy sterol (1) and four known sterols (2-5). Their planarstructures were defined by analyses of the spectroscopic data. The 27-nor-24-methylcholestan type side chain withan epidioxy nucleus (1) was unprecedented. Compounds 1-5 were isolated from a sponge Psammocinia sp. for the first time.

Efficient and Cost-Reduced Glucoamylase Fed-Batch Production with Alternative Carbon Sources

( Hong Zhen Luo ),( Han Liu ),( Zhenni He ),( Cong Zhou ),( Zhong Ping Shi ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2

Glucoamylase is an important industrial enzyme. Glucoamylase production by industrial Aspergillus niger strain featured with two major problems: (i) empirical substrate feeding methods deteriorating the fermentation performance; and (ii) the high raw materials cost limiting the economics of the glucoamylase product with delegated specification. In this study, we first proposed a novel three-stage varied-rate substrate feeding strategy for efficient glucoamylase production in a 5 L bioreactor using the standard feeding medium, by comparing the changing patterns of the important physiological parameters such as DO, OUR, RQ, etc., when using different substrate feeding strategies. With this strategy, the glucoamylase activity and productivity reached higher levels of 11,000 U/ml and 84.6 U/ml/h, respectively. The performance enhancement in this case was beneficial from the following results: DO and OUR could be controlled at the higher levels (30%, 43.83 mmol/l/h), while RQ was maintained at a stable/lower level of 0.60 simultaneously throughout the fed-batch phase. Based on this three-stage varied-rate substrate feeding strategy, we further evaluated the economics of using alternative carbon sources, attempting to reduce the raw materials cost. The results revealed that cornstarch hydrolysate could be considered as the best carbon source to replace the standard and expensive feeding medium. In this case, the production cost of the glucoamylase with delegated specification (5,000 U/ml) could be saved by more than 61% while the product quality be ensured simultaneously. The proposed strategy showed application potential in improving the economics of industrial glucoamylase production.

Research on Operation Performance of Women Oriented Cross-country Teaching in College: an Empirical Analysis Based on Multimedia Computer

Luo Hong,Long Xiaodong,Tao Ganchen,Long Jianjun,Wan Shaoyong,Chen Yuanping 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.9

With the development of computer multimedia technology, the new educational model, which is represented by the educational technology, has emerged in the field of education in our country. Sports’ teaching is a participatory teaching and learning activities, also, the current network resources is relatively abundant, so that design and development of a multimedia network teaching platform is very necessary. In this paper, we test the performance evaluation of directional cross-country teaching by using multimedia. Result shows that college students will have significant advantage after using teaching media, both in weight, vital capacity, sit ups, and 800 meters. After the experiment, experimental classes get higher score than comparative classes in 100 meters directional cross country ability test. The application of computer multimedia is helpful in physical education; it can improve students' learning enthusiasm and the effect is remarkable.

Pycnogenol attenuates atherosclerosis by regulating lipid metabolism through the TLR4–NF-κB pathway

Hong Luo,Jing Wang,Chenhui Qiao,Ning Ma,Donghai Liu,Weihua Zhang 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-

Atherosclerosis is a leading cause of death worldwide and is characterized by lipid-laden foam cell formation. Recently,pycnogenol (PYC) has drawn much attention because of its prominent effect on cardiovascular disease (CVD). However, its protective effect against atherosclerosis and the underlying mechanism remains undefined. Here PYC treatment reduced areas of plaque and lipid deposition in atherosclerotic mice, concomitant with decreases in total cholesterol and triglyceride levels and increases in HDL cholesterol levels, indicating a potential antiatherosclerotic effect of PYC through the regulation of lipid levels. Additionally, PYC preconditioning markedly decreased foam cell formation and lipid accumulation in lipopolysaccharide (LPS)-stimulated human THP-1 monocytes. A mechanistic analysis indicated that PYC decreased the lipid-related protein expression of adipose differentiation-related protein (ADRP) and adipocyte lipid-binding protein (ALBP/aP2) in a dose-dependent manner. Further analysis confirmed that PYC attenuated LPS-induced lipid droplet formation via ADRP and ALBP expression through the Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) pathway, because pretreatment with anti-TLR4 antibody or a specific inhibitor of NF-κB (PDTC) strikingly mitigated the LPS-induced increase in ADRP and ALBP. Together, our results provide insight into the ability of PYC to attenuate bacterial infection-triggered pathological processes associated with atherosclerosis. Thus PYC may be a potential lead compound for the future development of antiatherosclerotic CVD therapy.

Water photolysis at 12.3% efficiency via perovskite photovoltaics and Earth-abundant catalysts

Luo, Jingshan,Im, Jeong-Hyeok,Mayer, Matthew T.,Schreier, Marcel,Nazeeruddin, Mohammad Khaja,Park, Nam-Gyu,Tilley, S. David,Fan, Hong Jin,Grä,tzel, Michael American Association for the Advancement of Scienc 2014 Science Vol.345 No.6204

<P><B>The power of a pair of perovskites</B></P><P>In the past several years, perovskite solar cells have emerged as a low-cost experimental alternative to more traditional silicon devices. Luo <I>et al.</I> now show that a pair of perovskite cells connected in series can power the electrochemical breakdown of water into hydrogen and oxygen efficiently (see the Perspective by Hamann). Hydrogen generation from water is being actively studied as a supplement in solar power generation to smooth out the fluctuations due to variations in sunlight.</P><P><I>Science</I>, this issue p. 1593; see also p. 1566</P><P>Although sunlight-driven water splitting is a promising route to sustainable hydrogen fuel production, widespread implementation is hampered by the expense of the necessary photovoltaic and photoelectrochemical apparatus. Here, we describe a highly efficient and low-cost water-splitting cell combining a state-of-the-art solution-processed perovskite tandem solar cell and a bifunctional Earth-abundant catalyst. The catalyst electrode, a NiFe layered double hydroxide, exhibits high activity toward both the oxygen and hydrogen evolution reactions in alkaline electrolyte. The combination of the two yields a water-splitting photocurrent density of around 10 milliamperes per square centimeter, corresponding to a solar-to-hydrogen efficiency of 12.3%. Currently, the perovskite instability limits the cell lifetime.</P>

A STUDY ON CHINESE CONSUMERS' INTENTION TO PURCHASE SECONDHAND LUXURY GOODS

Hong Luo,Seong-Yeon Park 글로벌지식마케팅경영학회 2020 Global Marketing Conference Vol.2020 No.11

Mercury Resistance and Removal Mechanisms of Pseudomonas sp. Isolated Mercury-contaminated Site in Taiwan

Luo, Kai-Hong,Chen, Ssu-Ching,Liao, Hung-Yu Korean Society of Soil and Groundwater Environment 2016 지하수토양환경 Vol.21 No.5

A new strain of Pseudomonas sp. was isolated from mercury (Hg)-contaminated sites in Taiwan. This bacterium removed more than 80% of Hg present in the culture medium at 12 h incubation and was chosen for further analysis of the molecular mechanisms of Hg tolerance/removal abilities in this Pseudomonas sp. We used RNA-seq, one of the next-generation sequencing methods, to investigate the transcriptomic responses of the Pseudomonas sp. exposed to 60 mg/L of Hg²⁺. We de novo assembled 4,963 contigs, of which 10,533 up-regulated genes and 5,451 down-regulated genes were found to be regulated by Hg. The 40 genes most altered in expression levels were associated with tolerance to Hg stress and metabolism. Functional analysis showed that some Hg-tolerant genes were related to the mer operon, sulfate uptake and assimilation, the enzymatic antioxidant system, the HSP gene family, chaperones, and metal transporters. The transcriptome were analyzed further with Gene Ontology (GO) and Cluster of Orthologous Groups (COGs) of proteins and showed diverse biological functions and metabolic pathways under Hg stress.

A Generalized Formula for Elastic Shear Buckling of Trapezoidal Corrugated Web Girder

Hong-Guang Luo,Li-Ying Peng,Chan-Tao Zhang,Cheng-Xiu Cai 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.10

Trapezoidal corrugated web girder is widely used. The elastic shear buckling stress is an important parameter in the design and calculation of trapezoidal corrugated web girder. Accordingly, much research has been devoted on the analysis of typical elastic global shear buckling, elastic local buckling and elastic interactive shear buckling, and the corresponding formulae existing in a variety of literature. The results of finite element analysis suggest that there is a certain deviation in the calculated values based on classical elastic shear buckling formulae. The boundary conditions of corrugated web play an important role in the calculation of elastic shear buckling. And it is found that the shear buckling modes of the actual trapezoidal corrugated web may be called the atypical buckling, which is different from typical local buckling, global buckling and interactive buckling. The existence of the atypical buckling may result in some calculation deviations for the existing formulae of elastic shear buckling. Based on the classical elastic shear buckling formulae, a generalized formula for elastic shear buckling of trapezoidal corrugated web girder is obtained by numerical simulation. It intuitively reflects the relationships among various buckling modes. The formula has high calculation reference value and will promote the research of elastic shear buckling of trapezoidal corrugated web girder.

Study on Mutual Information Medical Image Registration Based on Ant Algorithm

Luo Hong-yan 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.9

The mutual information has been combined with medical image registration wildly, in order to improve the registration precision and speed of medical image, the application of ant algorithm on it is studied in depth. Firstly, the basic conception of mutual information is analyzed; secondly, the basic procedure of medical image registration is discussed; thirdly, the basic idea of ant algorithm is analyzed, and then the mutual information medical image registration based on FA is designed. Finally, simulation is carried out, and results show that this method can get better medical image registration effect.

A kinetic model for glycerol chlorination in the presence of acetic acid catalyst

Zheng-Hong Luo,Xiao-Zi You,Hua-Rong Li 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.1

A kinetic model for the chlorination of glycerol with hydrochloric acid in the use of acetic acid as catalyst is presented in this study. The model is based on a comprehensive chlorination mechanism, taking the formation of 1,3-dichloro-2-propanol and 2,3-dichloro-1-propanol into account while ignoring the formation of any intermediate in the chlorination system. Simulations were carried out under different chlorination conditions to calculate the concentrations of the main chemical species in the reaction system. The validity of the model was examined via a comparison of the calculated data with the experimental data on the chlorination of glycerol with hydrochloric acid at 363-393 K. The results show the model is capable of describing the chlorination performance with good agreement with experimental data.