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羅鳳鎭,李洸鎬,丁炳相,白相豪 최신의학사 1970 最新醫學 Vol.13 No.5
Under various status of fixation, washing, substrates and incubation, several conditions for an application of enzyme-labeled antibody method to localize LH in the rat pituitary gland were obtained and discussed. Bouin's solution could preserve endogenous LH most effectively after a longer duration of fixation and washing than that requires ordinarilly. Intense reactions of the specific LH cells were observed with 3, 3'-diaminobenzidine as a substrate, while a-naphthol and 4-Cl-l-naphthol showed lower intensity in either of specific cells or nonspecific backgrounds. The most and the only adequate concentration to reveal the intense staining was found at 0.001% of hydrogen peroxide in substrate solution. Residual of fixative chemicals in the tissue, higher concentration of hydrogen peroxide and longer incubation in substrate solution may act as a factor or factors to develop nonspecific staining of background tissue.
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나봉진 중앙대학교 의과대학 의과학연구소 1975 中央醫大誌 Vol.1 No.1
부신적출로 기능이 항진된 실험동물의 신장을 2.3% glutaraldhyde와 냉 1% osmium tetroxide 로 고정하고 Epon 812에 포매한 후 박절편을 만들어 전자현미경으로 경검하던 중 실험동물 M94 와 M87 에서 다음과 같은 상을 발견하고 이를 광범한 문헌조사를 통하여 고찰하였다. 1. 실험동물 M94 의 수입세동맥 내막내의 한 세포의 팽대된 조면형질내세망안에서 막에 쌓이지 않는 전자치밀한 낭중과립을 관찰하였다. 2. 실험동물 M87 의 Bowman 낭의 벽세포는 많은 막에 쌓인 과립을 포함하고 자유 ribosome 과 조면형질내세망은 소량 함유하고 있음을 관찰하였다. The renal tissues of mice of Swiss-Webster strain having been induced hyperactivity by adrenalectomy were fixed with 2.3% glutaraldehyde in 45mM cacodylate buffer and postfixed with cold 1% osmium tetroxide in 45mM cacodylate buffer, and then embedded in Epon 812 for electron microscopic study. During the previous experiment the unusual specific features of the juxtaglomerular cells of experimental animals No. 94 and No. 87 were observed, and then discussed through wide references published so far. 1. Animal No. 94 belonged to the group survived for one week after adrenalectomy and sacrificed at thirty minutes after cobalt nitrate injection. One of the juxtaglomerular cells in the media of the afferent arteriole showing markedly distended rough surfaced endoplasmic reticulum in which electron dense round granules without membrane was observed. 2. Animal No. 87 belonged to the group survived for four weeks after adrenalectomy and sacrificed at two hours after injection of cobalt nitrate. Some cells composing the parietal layer showed large round electron dense granules but small amount of free ribosomes and rough surfaced endoplasmic reticulum.
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羅鳳鎭 최신의학사 1971 最新醫學 Vol.14 No.6
Ninety primiparous Sprague-Dawley 'albino rats were irradiated once with the doses ranging from 50r to 500r on the 7th, 9th, and 11th day of gestation. Some of the rats which received 200r of irradiation were injected spleen homogenate intraperitoneally. Gross anomalies induced in fetuses, survival rates of fetuses, changes of fetal and maternal body weights, and numbers of corpus luteum of maternal ovary were studied on the 22nd day of gestation by hysterectomy and the following results were obtained. The gross anomalies appeared in the fetuses of experimental `groups were reduced tail, curled tail, abnormal position and growth retardation of the limbs, anencephalus, abnormal beak and ventral hernia. Throughout the anomalies the abnormal tail was observed in the highest incidence. The ninth day of gestation seems to be the most susceptible to the effect of irradiation in the critical period. The earlier irradiation and the larger doses of irradiation they received, the more obvious decrease of survival rate and body weight of fetuses were observed, and in those animal groups the reduction of rates of maternal body weight gain was also recognized. No direct relations between the numbers of corpus luteum and the irradiation were observed, and the spleen homogenate seemed not to be effective as a suppressor of the hazard of irradiation. The doses of 500r of X-irradiation induced maternal death or abortion in all cases.
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돼지 임파절의 섬유주 및 피질 성분을 중심으로한 조직학적 관찰
장가용,나봉진,백상호,이광호 中央醫學社 1971 中央醫學 Vol.20 No.4
As the experimental animals, the miniature pigs of Minnesota University were used. Germ-free piglets obtained by aseptic hysterectomy three to five days prior to term were kept in germ-free unit during the period of experiment. Those animals were sacrificed by bleeding and we took regional lymph nodes, that is mesenteric lymph node, cervical lymph node, sub maxillary lymph node and inguinal lymph node from them. Using hematoxylin and eosin staining, methyl-green pyronin staining and silver staining methods, we examined them under the microscope. Then we had results as following: 1. In the pigs, the trabeculae of the lymph node developed and branched into the lymph node connecting the capsule at the hilus of the lymph node; while in other species, the trabeculae of lymph node developed into the lymph node from many places of capsule of the lymph node. 2. Cortical substances including germinal center were developed following the trabeculae of the lymph node and medullary substances were located in the periphery of the lymph node. 3. The light zone of germinal center and small lymphocytes surrounding it placed themselves toward the near side of the trabeculae; on the other hand, the dark zone placed itself toward the far side of the trabeculae. 4. Those results suggested that the lymph flowed into lymph node through trabeculae or subtrabecular sinus in the pigs.
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李洸鎬,白相豪,羅鳳鎭,張家鏞 최신의학사 1971 最新醫學 Vol.14 No.8
As one of the series studying the effect of monosodium glutamate on the developing albino rat fetuses, the external abnormalities, body weight of fetuses, and increase of body weight and numbers of corpus luteum of mothers were observed. 41 primiparous Sprague-Dawley albino rats were injected monosodium glutamate intraperitoneally with the doses ranging from 1 to 3gm/kg of body weights on the 7th and 1lth day of estation. On the 22nd day of gestation, all the rats were sacrificed and compared with normal control groups. No specific external abnormalities were found on the 414 fetuses, but significant fetal weight increase in comparison with normal control groups were noticed. Twentyfive per cent of the pregnant rats delivered their litters on the 22nd day of gestation, that means the reduction of the gestation period. No differences, however, were observed between both of the body weights of fetuses of 22nd day of gestation and delivered newborns. Increasing rate in per cent of maternal body weight and numbers of corpus luteum showed no differences compared with the normal control groups.
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