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      • 錦江流域 淡水魚의 Sparganum에 關한 調査硏究

        辛大煥,羅榮彦,權斗星 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

        Prevalence of the plerocercoid larva in the fresh-water fishes in Keum-river basin was surveyed from April to September, 1986. To observe whether this plerocercoid larva is really sparganum or not, and can be transfered to the paratenic host or not, a study was performed with the adequate experimental animal (domestic fowl and cat). It was collected the 1096 fresh-water fishes that was ranged 8.0^-16.0cm in length. The results obtained were as follows; 1. A number of ova of Diphyllobothrium spp, was confirmed under the microscopic study, 5 days after experimental infection through the mouth, and them sacrificed to the experimental animal (final host, cat). The adult worm of Diphylobothrium spp., 9.5cm in length, was obtained and that worm was located in the upper position of rectum, Therefore, this plerocercoid larva was confirmed as Sparganum. 2. Transference to the paratenic host (dowestic fowl) was not resulted. 3. Among the total number of 1096 fresh-water fishes, only the Z. platypus was infected with 107 worms corresonding to 9.8%. 4. Infection rate according to the length of Z. platypus was 66. 4% at 12-13. 9cm in length, and this percentage was the highest prevalent rate.

      • Cortisone acetate 投與에 依한 Pneumocystis carinii 肺炎의 發症 課程

        閔庚璇,李英河,羅榮彦,辛大煥 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

        This study was undertaken to determine the provocative characteristics of experimental Pneumocystis carinii pneumonia in cortisone acetate treated rats. A Total of 55 male Sprague-Dawley rats, body weights 166-224g, were used. Five out of them were used as control group and remaining 50 were as experimental groups. In experimental groups, the rats were injected with 25mg of cortisone acetate twice a week for 10 weeks subcutaneously and drinking water contained 1000mg tetracycline hydrochloride per liter. Control group was not administered any drug. The 5 rats were sacrified weekly at each week in experimental group. but control group was sacrified at 10 weeks. For arterial blood gas analysis, blood was collected from carotid artery of rats using catheter. The rat's lung were removed after killed. The stamp smeared specimens of lungs were prepared and stained toluldin blue 0 for the quantification of P.carinii cysts. For the histopathologic observations, lungs were fixed in 10% buffered neutral formalin, cut into sections and stained with hematoxylin-eosin stain. For the histologic score of the intensity of P.carinii pneumonia, the sections were stained with Gomori's methenamine silver stain. The results obtained were as follows : 1. In the arterial blood gas analysis of cortisone acetate treated rats, blood pH and PaCO_2 was showed normal range. But PaO_2 was slightly decreased in experimental period. 2. Histopathologic finding of H-E staining sections were found in order of infiltration of lymphocyte and alveolar macrophage, eosinophilic foamy exudate, thickening of alveolar septum, desquamation of pneumocyte and fibrinoid degeneration and so on, but plasma cell was not found. 3. The histologic score of the intensity of P.carinii pneumonia was increased in proportion to the cortisonized periods of rats. 4. The number of cysts in the stamp smeared specimens of lungs were increased in proportion to the cortisonized periods of rats. And highly significant correlation was found between the histologic score of the intensity of P.carinii pneumonia and the number of cysts countered in the stamp smeared specimens of lungs(r=0.8620, ANOVA test p<0.0001). The present study revealed that provocative characteristics of experimental P. carinii pneumonia was highly significant relationship with the histologic score of P. carinii pneumonia and the number of cysts in the stamp smeared specimens of lung.

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