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Pseudomonas fluorescens 의 nahB 유전자의 분자적 클로닝과 E. coli 세포내에서의 발현
황경훈(Kyung Hoon Whang),최순영(Soon Young Choi),이나리(Na Ri Lee),민경희(Kyung Hee Min) 한국유전학회 2001 Genes & Genomics Vol.23 No.4
A gene encoding the cis-dihydrodiol naphthalene(DHN) dehydrogenase has been cloned from genomic DNA of Pseudomonas fluorescens SM11. The DNA fragment containing the nahB gene for the cis-DHN dehydrogenase was mapped with restriction endonucleases and sequenced. The DNA fragment contained an ORF of 780 bp encoding a polypeptide of 260 amino acid residues. The amino acid sequences of the enzyme revealed several conserved regions with those of the corresponding enzyme encoded in other strains of Pseudomonas. The homology of the amino acid sequence is 93% identity with that of the enzyme by P. putida OUS82. It is assumed that the cis-DHN dehydrogenase consists of four 28,800 molecular weight subunits and is specific for NAD as an electron acceptor for catalytic function.