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- 저자 : 한정호 ( Chung No Joo (검색결과 4 건)
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주충노,한정호 ( Chung No Joo,Jung Ho Han ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.1
It was attempted to observe in this study the effects of ginseng saponins, one ef the major components of Panax ginseng (C.A. Meyer) roots, on several TCA cycle enzymes such as succinate dehydrogenase(SDH), malate dehydrogenase (MDH) and α-hetoglutarate clehydrogenase (α-KGDH) in vitro and the following results were obtained. 1. The activity of SDH was found highest w-hen the concentration of the saponins in the assay mixture was 8.3 × 10^(-2)%, m-hich caused an increment of 37% of the control. However, SDH was inhibited when the concentration of the saponins in the reaction mixture was over 1.7 × 10^(-1). On the other hand, malonate inhibition of SDH was found recovered only slightly (about 10%), by the addition of the saponins, suggesting that a complete recovery of malonate inhibition of SDH can not be expected by the saponins. 2. The ginseng saponins were also found to activate MDH. When the concentration of the saponins in the assay mixture was 1.4×10^(-5)%, the activity of MDH was highest, 1.32 times that of the control. Again when the concentration of the saponins was over 1. 4 × 10^(-4)%, the MDH was inhibited, 3. α-KGDH was also activated in the presence of the saponins. It w-as found that the activity of α-KGDH was highest when the concentration of the saponins in the assay mixture was 1.5 × 10^(-3) %. 4. The oxidation of pyruvate was found to be increased by about 1.47 times that of the control. This was probably done through the activation of mitochondrial dehydrogenases by the saponins.
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인삼 사포닌의 생화학적 연구 ( Ⅶ ) 돼지의 염통 Isocitrate Dehydrogenase 에 미치는 영향
주중노,한정호 ( Chung No Joo,Jung Ho Han ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.4
It was attempted in this study to observe the effect of ginseng saponin, one of the major components of Panax ginseng roots on pig cardiac NADP^+ specific isocitrate dehydrogenase [ICDH(NADP^+), EC 1.1.1.42] and NAD^+ psecific isocitrate dehydrogenase [ICDH(NAD^+), EC 1. 1. 1.41] in the absence and presence of adenine nucleotides such as AMP, ADP, ATP and NADPH in vitro and the following results were obtained. 1) It was observed that the Michaelis constants (Km) of ICDH(NADP^+) for isocitrate in the presence of Mg^(++) and Mn^(++) under the experimental conditions were 4.5 × 10^(-5)M and 3.3 × 10^(-5)M respectively. 2) In the presence of Mg^(++), ICDH(NADP^+)-catalyzed reaction rate lowered in the presence of the saponin when the concentration of isocitrate was high (over 3.3 × 10^(-5)M) compared with Km but when the isocitrate concentration was low (below 1.0 × 10^(-5)M), moderate amounts of the saponin (8.3 × 10^(-5)%-8.3 × 10^(-4)%) stimulated the reaction significantly. A similar effect of the saponin was also observed at the ICDH(NADP^+) catalyzed reaction in the presence of Mn^(++). 3) It was observed that ADP(8.3 × 10^(-5)M) inhibited the ICDH(NADP^+) activity but its inhibition effect was not significant when the isocitrate concentration in the reaction mixture was high (3.3 × 10^(-4)M). The saponin (8.3 × 10^(-5)%) was found to lower the ADP inhibition at the low isocitrate concentration. AMP, ATP and NADPH inhibition of the ICDH(NADP^+) was also observed but the saponin was found unable to recover their inhibition of the enzyme activity. 4) It was realized that ADP was one of the cofactors for ICDH(NAD^+) catalyzed reaction and ATP inhibited this reaction. It was found that the saponin was not able to recover the ATP inhibition but stimulated the ADP activation of this enzyme. 5) It has been suggested that the ginseng saponin would behave amphoterically and its action might be dependent not only upon its own concentration but also upon the nature and concentration of substrate and effectors.
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인삼 Saponin의 생화학적 연구(IV) 말 및 닭의 간 Alcohol Dehydrogenase에 미치는 영향
주충노,윤병희,이상직,한정호,Joo, Chung-No,Yoon, Byung-Hee,Lee, Sang-Jik,Han, Jung-Ho 생화학분자생물학회 1974 한국생화학회지 Vol.7 No.3
한국산 인삼(Panax ginseng C. A. Meyer)의 한 성분군인 saponin 류의 말 및 닭의 간 alcohol dehydrogenase(ADH)에 미치는 영향을 분광학적으로 관찰함으로써 다음과 같은 결과를 얻었다. 1) 말과 닭의 간 ADH의 $K_m$ 값은 각각 $8{\times}10^{-4}M$ 및 $5.1{\times}10^{-4}M$이다. 2) ADH, ethanol의 농도를 일정하게 유지하고 saponin의 농도를 증가시키면 saponin 농도의 증가에 따라 ADH의 활성이 점차척으로 증가하나, 반응액에서의 saponin 류의 농도가 어느 이상(말은 $0.53{\times}l0^{-3}%$ 닭은 $0.27{\times}10^{-3}%$) 이 되면 ADH 활성은 오히려 감소되었다. 3) 반응액에서의 saponin 류의 농도가 $1.33{\times}10^{-5}{\sim}6.6{\times}10^{-5}%$ 일때의 말의 간 ADH의 반응 속도와 ethanol 농도와의 관계는 sigrnoid 형의 곡선으로 표시되었다. 4) 인삼 saponin 류의 농도 뿐 아니라, ethanol 농도에 따라서도 크게 다르며 반응액에서의 인삼 saponin 류의 농도가 $1.33{\times}10^{-5}%$이고 ethanol의 농도가 2~4mM 범위일 때 말의 간 ADH는 크게 활성화됨을 관찰하였다. The effect of ginseng saponins on equine and chicken hepatic alcohol dehydrogenases was investigated spectrophotometric ally and the following results were obtained. 1) The Km values of equine and chicken hepatic ADH were found to be $8{\times}10^{-4}M$ and $5.1{\times}10^{-4}M$ respectively. 2) When the concentration of ginseng saponins of ADH reaction mixture in the presence of known amounts of ADH and ethanol, the activity of ADH increased gradually as the concentration of the saponins increased. It was found, however, that the activity of the hepatic ADH decreased when the concentration of the saponins was over $0.53{\times}10^{-3}%$ (horse) and $0.27{\times}10^{-3}%$ (chicken) respectively. 3) The velocity-concentration curves of the ADH reactions in vitro in the presence of the saponins ranging from $1.33{\times}10^{-5}%$ were found to be sigmoid. 4) It was realized that the effect of the saponins on ADH activity was dependent not only upon its concentration but also upon the concentration of substrate and that the optimal concentrations of saponins and ethanol for the maximum activity of horse ADH were $1.33{\times}10^{-5}%$ and 2~4 mM respectively.
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인삼 Saponin 의 생화학적 연구 ( Ⅳ ) 말 및 닭의 간 Alcohol Dehydrogenase 에 미치는 영향
주충로,윤병희,이상직,한정호 ( Chung No Joo,Byung Hee Yoon,Sang Jik Lee,Jung Ho Han ) 생화학분자생물학회 1974 BMB Reports Vol.7 No.3
The effect of ginseng saponins on equine and chicken hepatic alcohol dehydrogenases was investigated spectrophotometrically and the following results were obtained. 1) The Km values of equine and chicken hepatic ADH were found to be 8 × 10^(-4)M and 5.1 × 10^(-4)M respectively. 2) When the concentration of ginseng saponins of ADH reaction mixture in the presence of known amounts of ADH and ethanol, the activity of ADH increased gradually as the concentration of the saponins increased. It was found, however, that the activity of the hepatic ADH decreased when the concentration of the saponins was over 0.53 × 10^(-3) % (horse) and 0.27 × 10^(-3) % (chicken) respectively. 3) The velocity-concentration curves of the ADH reactions in vitro in the presence of the saponins ranging from 1.33 × 10^(-5) % were found to be sigmoid. 4) It was realized that the effect of the saponins on ADH activity was dependent not only upon its concentration but also upon the concentration of substrate and that the optimal concentrations of saponins and ethanol for the maximum activity of horse ADH were 1.33 × 10^(-5) % and 2∼4 mM respectively.
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