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OsWRKY42 Represses OsMT1d and Induces Reactive Oxygen Species and Leaf Senescence in Rice
한무호,김치열,이준옥,이상규,전종성 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.7
We isolated a rice (Oryza sativa L.) WRKY gene which is highly upregulated in senescent leaves, denoted OsWRKY42. Analysis of OsWRKY42-GFP expression and its effects on transcriptional activation in maize protoplasts suggested that the OsWRKY42 protein functions as a nuclear transcriptional repressor. OsWRKY42-overexpressing (OsWR KY42OX) transgenic rice plants exhibited an early leaf senescence phenotype with accumulation of the reactive oxygen species (ROS) hydrogen peroxide and a reduced chlorophyll content. Expression analysis of ROS producing and scavenging genes revealed that the metallothionein genes clustered on chromosome 12, especially OsMT1d, were strongly repressed in OsWRKY42OX plants. An OsMT1d promoter:LUC construct was found to be repressed by OsWRKY42 overexpression in rice protoplasts. Finally, chromatin immunoprecipitation analysis demonstrated that OsWRKY42 binds to the W-box of the OsMT1d promoter. Our results thus suggest that OsWRKY42 represses OsMT1d-mediated ROS scavenging and thereby promotes leaf senescence in rice.
Modeling of a Transfer Function for Frequency Controlled Resonant Inverters
한무호,이치환,권우현 전력전자학회 2009 JOURNAL OF POWER ELECTRONICS Vol.9 No.4
A linear transfer function for the output current control of frequency-controlled resonant inverters is proposed in this paper. The circuit of resonant inverters can be transformed into two coupled circuits through the complex phasor transform. The circuits consist of cross-coupled power sources and passive elements. The circuits are used to induce the state space equation, which is transformed into the 4th order cross-coupled transfer function. The 4th order cross-coupled transfer function is modeled into a 2nd order linear transfer function based on a behavior analysis of the pole and zero locations that facilitate a simple and intuitive linear transfer function. The feasibility and validity of the proposed linear transfer function were verified by simulation and experiment.
한무호,박대섭,김경덕,피재호,최수민 한국잔디학회 2016 Weed & Turfgrass Science Vol.5 No.1
Zoysiagrass possessing the leaf yellowing with excessive tillers was first found in a golf course in Gyunggi province at last three to four years ago and since then have rapidly spread many golf courses in Korea. The symptom is very similar to that of yellow tuft or downy mildew, which is caused by Sclerophthora macrospora, on cool-season turfgrasses including Kentucky bluegrass and ryegrass. Microscopic study was firstly carried out to look over the presence of the pathogen in the infected leaf and stem tissues. Oospores and sporangia, reported as typical indicators of downy mildew, were obviously observed from the infected leaves. Using a set of primers based on the 18S ribosomal DNA from S. macrospora, the specific DNA fragment corresponding the gene was amplified. This study reveals that S. macrospora might be involved in spread of leaf yellowing of zoysiagrass with excessive tillers. 전국적으로 재배중인 한국잔디에서 확대되고 있는 총생잔디에서 현미경을 이용하여 피시움균의 난포자와 유사하지만 다소 큰 사이즈인 50~80 μm의 난포자를 관찰하였다. 동일 시료들을 암상태의 저온 다습한 조건에서 24시간 증식한 후 현미경으로 관찰한 결과 80~100 μm 크기의Sclerophthora macrospora 유주자낭이 확인되었다. 유전자 분석을 통한 확인을 위해 유전자 은행에 등록된 S. macrospora 염기서열 정보를 바탕으로 제작된 프라이머와 감염된 조직에서 추출한 DNA로 PCR 후, S. macrospora 특이적인 밴드가 증폭되는 것을 알 수 있었다. 이러한 결과로 S.macrospora가 한국잔디 총생 증상에 관여하는 가능성이 있을 것으로 사료된다.