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영지 (Ganoderma lucidum) 자실체 다당류의 분자량에 따른 생리활성
한만덕;김용현;이현수;권현정;전미애;한국일 순천향대학교 기초과학연구소 2010 순천향자연과학연구 논문집 Vol.16 No.2
The study was conducted to investigate the biological activities of an protein-bound polysaccharide (GLP) isolated from the fruit body of Ganoderma lucidum. GLP was extracted by hot water and then it was purified high (GLP-HM) and low (GLP-LM) molecular weight fraction by ethanol precipitation. Both of GLP-HM and GLP-LM at doses of 20 mg/kg in vivo had the ability to inhibit the tumor growth of Sarcoma 180 tumor-bearing mice. Inhibition rates of tumor (%) were inhibited 60.6% and 38.7% by GLP-HM and GLP-LM, respectively. In assay of anti-complementary activity in vitro, the GLP-HM were higher than that of the GLP-LM polysaccharide. And also, the GLP-HM enhanced nitric oxide production of mouse bone marrow cells. When the GLP-HM was injected intraperitoneally in tumor-bearing mouse at doses of 20 mg/kg, the concentration of TNF-α in vivo was 140.9 pg/ml in the serum, 269.6 pg/ml in sarcoma tumor mass and 879.4 pg/ml in liver. This study shows that the high molecular polysaccharide from mushroom can enhance more the bioactivities than that of the low molecular polysaccharide.
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