발효 상백피의 항산화활성 및 멜라닌 생성억제 효과

최은영 ( Eun Young Choi ),최태부 ( Tae Boo Choi ) 한국미용학회 2011 한국미용학회지 Vol.17 No.3

After extracting the mori cortex by using three methods of Hot Water Extraction, 70% Ethanol Extraction and Hot Water Extraction after Extracting the Mori Cortex with LM which is a lactobacillus, antioxidant activity increased by 1.9 times in case of fermented mori cortex as a result of measuring antioxidant activity by using DPPH, and changes of SOD-like activity increased according as fermentation progressed as a result of measuring SOD-like activity per specimen 0.2 mL and showed the highest values second day or third day after fermentation. Through this experiment, we decided to use the Mori Cortex after 3 days` fermentation. Also in the measurement of B16-F10 cell viability of the morus alba extracts fermented for 3 days, the cell viability hardly had cytotoxicity as more than 80% also in the treatment concentration of 300 μg/mL. As a result of measuring the antimelanogenic effect of fermented mori cortex in B16-F10 cell, melanin synthesis was impeded according to the treatment concentration of fermented morus alba extracts, and 100 μg/mL fermented morus alba extracts showed melanin-impeding effect also in the treatment concentration like arbutin used as the positive control group. As a field in fermented cosmetics through these experiments, Mori Cortex is expected to be able to be the basic data in developing a new product made by adding Chinese herb medicine, natural compounds as the raw materials of whitening cosmetics.

비스에칠헥실옥시페놀메톡시페닐트리아진(BEMT)을 봉입한 고형지질나노입자(Solid Lipid Nano-particle)의 화장품 응용

이근수 ( Geun-soo Lee ),이동환 ( Dong-whan Lee ),표형배 ( Hyeung-bae Pyo ),최태부 ( Tae-boo Choi ) 대한화장품학회 2007 대한화장품학회지 Vol.33 No.4

비스에칠헥실옥시페놀메톡시페닐트리아진(Bis-ethylhexyloxyphenolmeth oxyphenyltrizine; BEMT)은 식품의약품안전청 고시 기능성 원료로 자외선 차단 제품에 널리 사용되는 UVA와 UVB의 화학적 자외선 흡수제이다. 그러나 BEMT는 실제 적용에 있어 여러 가지 결점이 있어 사용이 제한되고 있다. 본 연구의 목적은 BEMT가 적용된 고형지질나노입자(BEMT- SLN)의 자외선차단제품 응용에 있다. 제조된 고형지질나노입자의 입도는 약 330 nm, 봉입율은 93.3 %, 결정화지표는 4.3 %였다. In vitro 방출 및 투과 실험 결과에서, BEMT는 SLN보다 O/W 에멀젼이 대체로 높았다. In vivo 실험에서 SLN의 BEMT 방출비는 80 % 감소하였다. 또한 in vitro UV 방어 효과 실험에서 SLN이 적용된 처방의 자외선방어지수(SPF) 값은 약 2.5배 증가하였다. 결국 SLN은 BEMT를 효과적으로 봉입하고 있었으며, 자외선 차단 상승 효과를 나타낸다. Bis-ethylhexyloxyphenolmethoxyphenyltrizine (BEMT) is one of the most widely used chemical UVA + UVB double absorbers in sunscreen products. But topical application of BEMT is restricted due to its defects in product. The purpose of this study is to adopt the sunscreen product of solid lipid nano-particles containing BEMT (BEMT-SLN). The particle diameters, the encapsulation efficiencies and the crystallization index (C.I.) are about 330 nm, 93.3 % and the 4.3 %. As a result, in vitro penetration and release of BEMT were generally higher in O/W emulsion than the SLN formulation. However in vivo study, it was shown that the rate of release could be decreased by 80 % in the SLN formulation. The sun protection factor (SPF) of the SLN formulation increased by 100 % in the in vitro UV protection test. Therefore, SLN formulation potentiated the UV-blocking power of BEMT. This study suggest that SLN can be used for the encapsulation of BEMT.

연구발표논문초록 ( 생물화공 (Ⅰ) ) : 이중실관 생물반응기에서의 하이브리도마 세포의 배양특성

오덕재(Duk Jae Oh),장호남(Ho Nam Chang),최태부(Tae Boo Choi) 한국화학공학회 1988 춘계학술발표회 Vol.- No.-

위벽세포에 대한 헬리코박터 파이로리의 부착 및 탈착에 관한 연구

구재경,최태부 건국대학교 산업기술연구원 1999 건국기술연구논문지 Vol.24 No.-

H. pylori를 닭에 면역접종을 하여 생산된 난황 항체는 면역접종을 하지 않은 군에 비해 10배 이상의 H. pylori에 대한 특이항체가 생산되었다. 난황 항체를 λ-carrageenan을 이용하여 정제한 결과 약 70-75% 정도의 순도로 조정제되었다. H. pylori가 위 상피세포에 결합하는 현상이 H. pylori의 감염에 중요한 첫 단계라는 점에 착안하여 H. pylori의 위벽세포에 대한 결합의 특성을 조사하는 한편, 난황 항체 및 어려가지 부착억제후보물질을 이용하여 H. pylori의 부착 억제 및 탈착을 할 수 있는지에 대하여 실험하였다. 부착의 특성은 positive-cooperative한 결합을 하는 것으로 나타났으며 부착억제 실험의 결과, 0.5mg/ml의 난황 항체를 이용하였을 때 AGS에 대한 H. pylori의 부착을 90% 가량 저해하거나 AGS에 이미 부착된 H.pylori를 80%이상 탈착시킬 수 있었다. 그러나 난황 항체에 의한 H. pylori의 부착억제나 탈착은 균주에 따라 그 효과가 달라지므로 앞으로 이를 보완하는 실험이 필요하다. 부착억제 후보 물질의 탐색결과, GPI 및 dextran sulfate 등 몇 가지 유용한 후보 물질의 선별이 가능하였다. 하지만 앞서 기술한 바와 같이 H. pylori의 결합에 관계하는 receptors 및 molecules가 어떤 특정한 한가지가 아니라 여러 가지의 복합적인 물질이 관여 하는 것이기 때문에 여기에 대해서는 더 많은 연구가 뒷받침되어야 할 것으로 사료된다. H. pylori known as a key pathogen for chronic gastric and duodenal ulcers. Egg yolk antibody, IgY produced from chicken immunized with H. pylori antigen was tested for the inhibition of adhesion of H. pylori to gastric epithelial cell, AGS, and for the detachment of H. pylori adhered to AGS in vitro. We also characterized the pattern of H. pylori adherence to AGS cell line, which was positive-cooperative interaction. In the adhesion inhibition and detachment study, the inhibition of H. pylori adhesion to AGS was as high as 90% using 0.5mg/ml of antibody only and detachment was also achieved almost 80%. Inhibitory effect of adhesion-inhibiting candidates was investigated, and GP1 and dextran sulfate was very effective. However, this effect was severely dependant on the H. pylori strains tested. Further studies are necessary to employ the egg yolk antibodies for the treatment of H. pylori in vivo.

Methanol 자화성 효모에 의한 균체 단백질 생산

권태종,이동희,정호권,최태부 건국대학교 1992 學術誌 Vol.36 No.2

SCP producing yeast JAM -3032 that has high productivity potential with methanol as carbon and energy source was isolated from soil, and conditions for SCP production, nutritional values, and alcohol oxidase of the microorganism were investigated. Isolated strain JAM-3032 was identified as Pichina on the morphological, cultural, physiological and biochemical characteristics. The production of cell mass reached at maximal level after 54 hrs cultivation at 28℃, pH 6.0 in the medium containing methanol 3.0%, yeast extract 0.8%, K2HPO4 0.1%, KH2PO4 0.1%. MgSO4ㆍ7H2O 0.05%, alanine 0.01%, nicotinic acid 0.04%, and biotin 0.04%. Dry cells of Pichia sp. JAM -3032 was composed of proteins 53.3%, carbohydrates 31. 5%, nucleic acids 5.6%, lipids 2.9%, ash 6.4% and amino acid composition of the proteins was satisfied in FAO reference. Biosynthesis of the alcohol oxidase of the strain JAM -3032 was induced by methanol. The enzyme was purified by treatment of ammonium sulfate fractionation, DEAE-Sephadex A-50 ion exchange chromatography and Sepharose 6B gel nitration. The purified enzyme was homogeneous on SDS-PAGE, and molecular weight was estimated to be about 520,000 D that was consisted of 8 subunits containing FAD as coenzyme. The optimal pH and temperature for the enzyme reaction was 7.5 and 37℃, respectively. Activation energy was 17.1Kcal/mol and half inactivating temperature was 40℃. The enzyme activity was inhibited by Ag+, Cu2+ and Hg2+ ions remarkably. The enzyme acted on methanol specifically, and its Km value was 0.89 mM.

Streptomyces sp. LAM 90-2가 생산하는 광범위 항생물질의 부분 정제 및 특성

강상모,정선호,권태종,최태부,이동희 건국대학교 산업기술연구원 1997 건국기술연구논문지 Vol.22 No.-

Candida albicans IFO 0583에 대하여 항진균활성을 갖는 Streptomyces sp. LAM 90-2를 토양으로부터 분리 하였다. 배양여액을 Amberlite XAD-4, DEAE-cellulose column chromatography, 1차, 2차 Sephadex LH 20 column chromatography로 정제하여 n-butanol:acetic acid:water(2:1:2)를 전개용매로 하여 silicagel gel 60 plate( Merck)로 TLC를 실시한 Rf치는 0.64였다. 본 항생물질은 U.V. scanning시 275nm에서 최대 흡수파장을 보여주었으며, pH 4-10, 60℃에서 안정하였고 여러 가지 용매에 대한 본 항생물질의 용해성을 조사한 결과 water, acetic acid, butyl acetate 등에 잘 녹고 methanol, ehthyl- acetate 등에 약간 녹았으나 butanol, chloroform 등에는 녹지 않았다. 이 항생물질은 곰팡이(Microsporum gypseum, Fusaruim solani, etc), 효모(Candida albicans, Sacch cerevisiae, etc), 그리고 세균(B. subtilis, E. coli, etc) 에 대해 광범위한 항균 spectrum을 나타냈다. A strain of Streptomyces sp. LAM 90-2 producing broad spectrum antibiotic was isolated from soil. The antibiotic was purified from cultured filtrate by the adsorption of Amberlite XAD-4 and DEAE-celloulose followed by the twice Sephadex LH-20 column chromatography. The Rf value was 0.64 of silica gel 60 plate with n-butanol: acetic acid: water(2:1:2) system as developing solvents. The purified antibiotic was stable for 2hrs at 60℃, and the pH of range from 4 to 10. This antibiotic was easily dissolved in water, acetic acid, and butyl acetate but not in butanol, chloroform, methanol, and ethyl acetate. The biochemical tests of the antibiotic appeared to be positive in Benedict, Bial but to be negative in Ehrlich, ninhydrin reaction. This antibiotic showed the broad antimicrobial spectrum against fungi(Microsporum gypseum, Fusarium solani, etc), yeasts(Candida albicans, Saccharomyces cerevesiae, etc), and becteria(B. subtilis, E. coli, etc).