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문미연(Mi-Yeon Moon),이하연(Ha-Yeon Lee),장문식(Moon-Sik Chang),차준석(Joon-Seok Cha) 한국화장품미용학회 2018 한국화장품미용학회지 Vol.8 No.1
The extracts of Trachelospermum asiaticum were examined for the inhibitory effect on the melanin synthesis in B16/F10 mouse melanoma cells, to evaluate as a functional ingredient for cosmetics. Similar to the effect of arbutin at 50 μg/mL (38%), 70% ethanol extracts, chloroform and ethyl acetate layers showed 34%, 52% and 45% of inhibition at 100 μg/mL. Investigation of constituents from the ethanol extracts of T. asiaticum led to the identification of six compounds; arctigenin (1), matairesinol (2), arctiin(3), luteolin 7-O-glucoside (4), quercitrin (5), luteolin 4`-O-glucoside (6). The compound (5) was isolated from T. asiaticum for the first time, and more interestingly, compounds 1∼3 showed 58%, 84% and 14% inhibitory effect of melanin synthesis. Based on these resutls, we can propose T. asiaticum is a natural cosmeceutical ingredient with skin brightening effect.
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정선영(Seon-Young Jeong),황혜리(Hai-Li Hwang),유은경(Eun-Kyung Ryu),차준석(Joon-Seok Cha) 한국화장품미용학회 2018 한국화장품미용학회지 Vol.8 No.2
This study is for evaluating red kohlrabi (Brassica oleracea var. gongylodes) sprouts as cosmetic ingredient. The sprouts were grown under different light conditions: white, red, blue and dark. We examined biological activities of sprouts extracts to reveal anti-oxidant, anti-inflammatory and skin-brightening effects. In DPPH radical scavenging assay, sprouts grown under the white light showed the best performance, demonstrating the optimized cultivation of raw materials. In melanin synthesis inhibition assays, red kohlrabi sprouts extracts showed concentration-dependent activity, suggesting skin whitening efficacy as a cosmetic ingredient. More interestingly, the extracts showed significantly high activities of NO suppression in macrophages, which is implicated in anti-inflammatory agents of both cosmetic and pharmaceutical industries. Decrease of NO synthesis was up to 80% in our tests, and we subsequently elucidated that TNF-α is reduced by the extracts, leading to the reduction of NO. Light conditions did not seem to modulate skin whitening and anti-inflammatory efficacies, but we could conclude that white light is the best condition given that antioxidant activity was significantly better than the other 3 conditions. With these results, we uncovered the beneficial effects of red kohlrabi sprouts extracts as a cosmetic ingredient, and demonstrated that plant factory system with different light conditions can serve to supply industry-optimized raw materials.
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문미연(Mi-Yeon Moon),이하연(Ha-Yeon Lee),장문식(Moon-Sik Chang),차준석(Joon-Seok Cha) 한국화장품미용학회 2018 한국화장품미용학회지 Vol.8 No.1
We identified active components from the methanol extract of Opuntia ficus-indica seeds. Five compounds were identified: 9‘-O-methyl isoamericanol A (1), americanol A (2), 3,3‘-bisdemethylpinoresinol (3), isoamericanin A (4) and adenosine (5). We evaluated the antioxidant, anti-inflammatory and melanogenesis inhibitory effects of these compounds. In antioxidant test, compounds 2, 3, 4 and 5 showed 12-47% of ROS generation inhibition at the concentration of 100 μg/mL. In NO assay, compounds 2 and 3 are similar with the positive control, quercetin by displaying IC<SUB>50</SUB> of 57.80, 57.27, and 51.44 μg/mL, respectively. Furthermore, melanogenesis inhibitory effects of compounds 2, 3, and 4 were 3 to 5 times higher than arbutin, a well-known skin whightening agent. These results indicate that Opuntia ficus-indica seed can be an effective natural cosmetic ingredient.
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