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RISS 인기검색어
- 검색키워드
- 저자 : 주충노 ( Kwang Keun Oh (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Effect of Some Amphiphiles Including Ginseng Saponin on Adenylate Cyclase System
오광근,주충노,Oh, Kwang-Keun,Joo, Chung-No 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.4
인삼 사포닌 분획과 Lubrol-PX, Triton X-100, SDS와 같은 계면 활성을 가진 amphiphile이 membrane에 존재하는 adenylate cyclase system에 미치는 영향을 연구함으로써 인삼 사포닌의 비특이적인 효소 활성화 작용이 계면 활성에 기인한다는 것을 다른 amphiphile과 비교 검토하였다. 본 연구 결과로부터 인삼 사포닌 분획에 의한 adenylate cyclase의 활성증가가 plasma membrane receptor에 결합하는 epinephrine의 양의 증가 때문인지 또는 adenylate cyclase system의 receptor외의 부분에 대한 작용 때문인지는 확실치 않으나 인삼 사포닌의 작용이 다른 계면 활성 물질인 Lubrol-PX, Triton X-100, SDS와 유사한 것으로 보아 인삼 사포닌의 계면 활성이 adenylate cyclase system의 활성화에 중요한 역할을 할 것이고 membrane의 구조를 변화시킴으로써 epinephrine의 receptor에 대한 결합이 용이하여 adenylate cyclase의 활성이 증가된 것으로 해석된다. Effect of some amphiphiles on membrane have been investigated by using adenylate cyclase system of rat plasma membrane. The maximum activity of adenylate cyclase system was at $10^{-5}\;M$ of epinephrine, and epinephrine was found to bind rat hepatic plasma membrane selectively. Epinephrine binding to rat hepatic plasma membrane was maximum when the concentrations of ginseng saponin fraction, Lubrol-PX, Triton X-100 and sodium dodecyl sulfate (SDS) were $10^{-4}%$, $10^{-2}%$, $10^{-1}%$ and $10^{-3}%$ respectively. Adenylate cyclase activity was stimulated in the presence of the above amphiphiles and the maximum activity was observed when the concentrations of ginseng saponin fraction, Lubrol-PX, Triton X-100 and SDS were $10^{-5}%$, $10^{-1}%$, $10^{-4}%$ and $10^{-3}%$ respectively. Uptake of ginseng saponin by rat hepatic plasma membrane was found about 27% of the added ginseng saponin fraction.
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Adenylate Cyclase System 에 대한 인삼 사포닌 및 몇가지 Amphiphile 의 영향
오광근,주충노 ( Kwang Keun Oh,Chung No Joo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.4
Effect of some amphiphiles on membrane have been investigated by using adenylate cyclase system of rat plasmamembrane. The maximum activity of adenylate cyclase system was at 10^(-5) M of epinephrine, and epinephrine was found to bind rat hepatic plasma membrane selectively. Epinephrine binding to rat hepatic plasma membrane was maximum when the concentrations of ginseng saponin fraction, Lubrol-PX, Triton X-100 and sodium dodecyl sulfate (SDS) were 10^(-4)%, 10^(-2)%, 10^(-1)% and 10^(-3)% respectively. Adenylate cyclase activity was stimulated in the presence of the above amphiphiles and the maximum activity was observed when the concentrations of ginseng saponin fraction, Lubrol-PX, Triton X-100 and SDS were 10^(-5)%, 10^(-1)%, 10^(-4)% and 10^(-3)% respectively. Uptake of ginseng saponin by rat hepatic plasma membrane was found about 27% of the added ginseng saponin fraction.
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