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HaCaT 각질형성세포에서 자외선 B에 의하여 유도되는 염증성 사이토카인에 대한 Curcumin의 항염증 효과
조재위 ( Jae We Cho ),이규석 ( Kyu Suk Lee ) 대한피부과학회 2009 대한피부과학회지 Vol.47 No.2
Background: UVB irradiation induces acute inflammation through expressions of IL-6, IL-8, and TNF-α, in keratinocytes. Curcumin, extracted from the rhizomes of Curcuma longa, has been described as having anti-inflammatory properties; however, it still unknown as to whether curcumin inhibits the UVB-induced expressions of IL-6, IL-8, and TNF-α in HaCaT cell lines. Objective: In this study we investigated the effects of curcumin on the expression of IL-6, IL-8, and TNF-α in HaCaT cells, as well as the photoprotective effect of curcumin ointment in UVB-irradiated hair less mice. Methods: HaCaT cells were irradiated with various doses of UVB (0, 100, 200, 300 mJ/cm2). Total RNA was extracted from the cells and gene expression was confirmed by RT-PCR, EMSA, and Western blot analysis. In addition we examined whether the ointment containing curcumin prevents the photo damaging events in hairless mice. Results: UVB-irradiated HaCaT cells clearly showed an increased expression of IL-6, IL-8, and TNF-α in a UVB dose-dependent manner (0, 100, 200, 300 mJ/cm2). Interestingly, pretreatment of curcumin (40 uM) dramatically reduced the expressions of UVB-induced IL-6, IL-8, and TNF-α. In addition, activation of NF-κB and MAPKs (p38, JNK, ERK) by UVB were partially attenuated by pretreatment of curcumin in HaCaT cells. Furthermore, the application of ointment containing curcumin partially protected the erythema and skin atrophy in UVB-irradiated hairless nude mice. Conclusion: Collectively, these results indicate that curcumin may be applied as a promising anti-inflammatory natural agent through modulation of inflammatory cytokines such as IL-6, IL-8, and TNF-α. (Korean J Dermatol 2009;47(2):121∼126)
섬유아세포에서 민들레 추출물 함유제(AF-343)에 의한 제 1형 교원질 발현 증가
조수묵(Soo Muk Cho),김재현(Jae Hyun Kim),김종근(Jong Keun Kim),박기문(Ki Moon Park),조호찬(Cho Ho-Chan),김인수(In Su Kim),김범준(Beom Joon Kim),천영진(Young-Jin Chun),조재위(Cho Jae We) 대한약학회 2012 약학회지 Vol.56 No.1
We previously reported that the extract of Taraxacum platycarpum (AF-343) had several biological properties such as skin hydration and anti-inflammatory effects, thereby AF-343 be a promising anti-atopic dermatitis agent. However, few studies have been conducted to evaluate its effect on modulation of extracellular matrix proteins in human skin fibroblasts. The purpose of this study was to investigate the expressions of type I collagen, MMP-1, Smad2/3, and TIMP-1 proteins in AF-343-treated human skin fibroblasts. Human skin fibroblasts were treated by various concentrations of AF-343 (0~2 mg/ml). The expressions of type I collagen, matrix metalloproteinase-1 (MMP-1), Smad2/3, and TIMP-1 proteins were analyzed by Western blot analysis. In addition, level of type I collagen mRNA was analyzed by CAT assay. Expression of type I collagen protein was increased in AF-343-treated human skin fibroblasts by dose and time-dependent manners. Consistent with this result, the expressions of phospho-Smad2/3 in skin fibroblasts were increased and MMP-1 expression was decreased by AF-343 treatment. TIMP-1 expression was not significantly changed in AF-343 treated skin fibroblasts. Extract of Taraxacum platycarpum (AF-343)-induced up-regulation of type I collagen expression was through increased expression of phospho-Smad2/3. These results were occurred combined with down-regulation of MMP-1 in skin fibroblasts. Taken together, this study indicated that AF-343 has property of the modulation of ECM in tissue as well as skin hydration and anti-inflammation.
조재위 ( Jae We Cho ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Psoriasis is a chronic inflammatory skin disease, characterized by a combination of abnormal proliferation of keratinocytes, immunology, and vascular proliferations. During the disease progression such as papules/patches/plaques, some key molecules play roles on lesional maturation. Some key molecules are secreated from keratinocytes, T cells, and vessels. Proteomics have revealed some clues regarding disease maturation of psoriasis. In the present study, I conducted an investigation of different proteomes of psoriatic lesional skin(papules and plaques), which is compared with normal and nonlesional psoriatic skin. Proteomes showed differential expression of 104 and 160 protein spots in papules and plaques psoriatic skin, respectively. Protein spots were categorized as proteins with involvement in negative regulation of apoptosis, defense response-related proteins, and inflammatory response. However it is difficult to discriminate clearly between papules and plaques of lesional psoriatic skin. Taken together, multiple proteins, including regulation of cell death, defense response, inflammatory response, and ROS regulation, play roles on disease maturation, and these roles seems to be overlapped between papules and plaques.
조재위 ( Jae We Cho ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
The exact mechanism by which genetics, inflammation, and mechanical tension promoting scarring have been suggested by several study. The surgical hypertrophic or keloid scar is occurred basically by patient-related factors (age, skin type, genetics, population, and hormonal imbalances) and topography-related factors, and environmental factors (type of surgery, inflammation, and skin or wound tension). On the aspect of cellular and molecular mechanisms, some studies have shown that fibroblast is one of the most important element in the process of scar formation, while epidermal and endothelial cells exert synergistic effects as well. Up-regulation of TGF-β1 and TGF-β2, Smad 2/3, and IL-6 can lead to excessive scarring as demonstrated in numerous in vitro and in animal models. Recently, three-dimensional (3-D) structures of dermal tissues act as a template to modulate cell functions that are essential the regeneration of skin structure and function. Loss of the dermal tissue integrity and continuity due to trauma leads to a lack of the template effect, which may be one important mechanism that hinders the recovery of cell function, resulting in scar formation. Thus, scar formation are mediated by complicated factors such as patient-related factors, topography-related factors, environmental factors, cytokines (TGF-β/Smad, IL-6) as well as loss of 3-D template effect.
조재위 ( Jae We Cho ),김창욱 ( Chang Wook Kim ),이규석 ( Kyu Suk Lee ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.9
Congenital midline cervical cleft (CMCC) is a rare congenital anomaly of the ventral of the neck. The CMCC consists of a skin tag, mucosal surface, and a caudal sinus in the ventral midline of the neck. Embryologically, the development of CMCC is thought to be mainly due to failure of the branchial arches to fuse in the midline, resulting in a ventral midline defect of the skin of the neck. We report a rare case of congenital midline cervical cleft in a 6-year old girl, plus a review of the related literature. (Korean J Dermatol 2006;44(9):1088~1090)
황금추출물의 추출 방법에 따른 세포 독성 평가 및 피부 안전성 평가
김형미 ( Hyeong Mi Kim ),임윤영 ( Yun Young Lim ),조수묵 ( Soo Muk Cho ),김민영 ( Min Young Kim ),손인평 ( In Pyeong Son ),석장미 ( In Pyeong Son ),박진오 ( Jin Oh Park ),박종호 ( Jong Ho Park ),조재위 ( Jae We Cho ),김범준 ( Beom 대한피부과학회 2012 대한피부과학회지 Vol.50 No.11
Background: Scutellaria baicalensis Georgi extract is used as a traditional herbal medicine. The efficacy of Scutellaria baicalensis Georgi extract is known for antioxidative activity, antiinflammation effect, antibacterial effect, inhibitory effect of melanin synthesis, sun protection effect, antiallergy effect, and etc. Objective: We confirmed the cell viability or inhibitory effect of melanin synthesis in HaCaT (human keratinocyte cell line) and B16F10 (murine melanoma cell line) cells and the skin safety test through a clinical test (dermal irritation study) for Scutellaria baicalensis Georgi extract, according to the extraction methods. Methods: We checked the cell viability, using MTT assay and inhibitory effect of melanin synthesis in B16F10 cells or HaCaT cells for thirty one Scutellaria baicalensis Georgi extract, according to the extraction methods. Then, we evaluated the skin safety for selected eight Scutellaria baicalensis Georgi extract through a primary dermal irritation test. Results: Among the thirty one Scutellaria baicalensis Georgi extracts, according to the extraction methods, we selected eight Scutellaria baicalensis Georgi extracts that were not detected with cell toxicity in HaCaT cells and B16F10 cells, and could have inhibited the melanin synthesis in B16F10 cells. The selected eight Scutellaria baicalensis Georgi extracts identified the skin safety through a primary dermal irritation test. Conclusion: We expect clinical trials for whitening efficacy based on inhibitory effect of melanin synthesis and human skin safety for Scutellaria baicalensis Georgi extracts. (Korean J Dermatol 2012;50(11):959∼968)