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우인옥(In-Ohk Ouh),이주연(Ju-Yeon Lee),문서영(Seo Young Moon),송재영(Jae Young Song),장상호(Sang Ho Jang),정광면(Kwang Myun Cheong),박최규(Choi-Kyu Park),이윤희(Yoon-Hee Lee) 한국예방수의학회 2021 예방수의학회지 Vol.45 No.4
A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.