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잎새버섯(Grifola frondosa)의 균사체와 다당류 생산을 위한 최적 배양조건
전한울 외 순천향대학교 기초과학연구소 2011 순천향자연과학연구 논문집 Vol.17 No.2
The influences of pH and medium composition on mycelial growth and exopolysaccharide (EPS) production of Grifola frondosa were investigated in shake flasks. The optimal initial pH for mycelial growth and ESP production were found to be 4.0. The optimal carbon and nitrogen sources were brown sugar (0.33 g/100 ㎖) and skim milk(0.52 g/100 ㎖), respectively. Under the optimized conditions, a mycelial biomass of 0.65 g/100 ㎖ and an EPS yields of 0.075 g/100 ㎖ were obtained in 7 days. The results strongly suggest that EPS production from G. frondosa is growth-associated. After submerged culture in optimal culture medium, the polysaccharide from the culture broth were extracted and purified from mycelial culture broth by ethanol precipitation method. In vitro cytotoxicity tests, crude polysaccharide were not cytotoxic against macrophage cell line, such as Raw 264.7 at the concentration of 10 ug/㎖. EtOH extracted-polysaccharide generated 24.6 uM of nitric oxide (NO) when cultured with Raw 264.7 cell at the concentration of 1 ug/㎖. Therefore, the results suggest that G. frondosa-derived β-glucan from new synthetic medium (LBL) may exert its immunostimulating activities with similar potency to LPS.