수영강의 (水營江) 저서성 (底棲性) 대형무척추동물에 (大型無脊椎動物) 관한 생태학적 연구 -Ⅱ. 본류와 (本流) 4개 지류에서의 (支流) 분포와 풍부도에 관한 조사 -

권태성,전태수 ( Tae Sung Kwon,Tae Soo Chon ) 한국하천호수학회 1991 생태와 환경 Vol.24 No.3

Physico-chemical environments and the distribution and abundance of the benthic macroinvertebrates were investigated seasonally in the Suyoˇng river from October 1988 to July 1990. In total one hundred and thirteen species were collected during the study period. Chironomids consisting of thirty six species, were most diverse among the macroinvertebrates in the Suyoˇng river. In the clean regions Cricotopus sp., Microspectra sp. and Diamesa sp. were dominant while Limnodrilus hoffmeisteri and Chironomus sp. were abundant in the lower polluted regions. A pollution-tolerant Polychaeta species, Capitella capitata complex, was sampled in the river mouth region. Sediment compositions, current velocities and food resources, which are important in controlling the densities of the benthic invertebrates, showed close relationships with organic pollutions in the Suyoˇng river.

수영강의 (水營江) 저서성 (底棲性) 대형무척추동물에 (大型無脊椎動物) 관한 생태학적 연구 - Ⅲ. 화학 및 생물학적 지수를 이용한 수질분석 -

나태성,전태수 ( Tae Sung Kwon,Tae Soo Chon ) 한국하천호수학회 1993 생태와 환경 Vol.26 No.2

Based on environmental data and benthos collected in the Suyoˇng river from October in 1989 to July in 1990, water qualities were estimated using biological and chemical indices such as Trent Biotic Indes(TBI), Biotic Score(BS), Diversity Index(H), Tsuda Index(TS), BOD, and etc.. Overall the two upper reaches, including the Choˇlma and Suyoˇng stream, were in clean-to slightly-enriched-states, mainly ranging from oligosaprobity to β-mesosaprobity, while most locations of the lower reaches and the downstream were in moderate-to heavily-enriched-states, ranging from α-mesosaprobity to iso-saprobity. Although observed values of BOD were generally compatible with biological indices in the surveyed area in overall terms, there were some cases of differences between these indices. In the clean or slightly enriched states when there were environmental disturbances, such as occurrence of suspended solids, flooding, etc., biological indices appeared to reflect the effect of environmental disturbances more sensitively than BOD. Amenities measured in this study were also shown that they could be compatibly used with other indices.

바퀴, Blattella germanica (Linnaeus),개체군의 크기와 발육속도를 예측하기 위한 온도의존 Simulation Model의 구성

權太晟,全胎秀 부산대학교 환경문제 연구소 1985 環境硏究報 Vol.3 No.-

By using temperature as a key variable, a simulation model was constructed to predict the size and development speed of the German cockroach population through 3 research steps. First, data for developmental units and survival rates at each life stage were obtained through rearing experiments at five different temperatures. Secondly, biological parameters needed for modeling were obtained based on these rearing results. And thirdly a basic life model was constructed in a simulative framework in FORTRAN for predicting populatiion development on the individual basis. To save memory space in computer programming, two files were utilized in translocating individual informations each other as time proceeded. The developed simulation model showed flexibility and applicability in representing insect life system and could be efficiently used as strategic tool in managing the cockroach population.

부산의 영세 음식점과 밀집가옥에서의 바퀴벌레 발생조사

全胎秀,權太晟 부산대학교 1984 자연과학논문집 Vol.38 No.-

Cockroaches were collected by sticky traps at the kitchen and the room of small restaurants and densly-located houses in Pusan from July, 1983 to January, 1984. In summer, the German cockroach, Blattella germanica, was most abundant. The American and smokey brown cockroaches were also collected in a smaller number. The abundance of the German cockroach showed 4 patterns, such as peaking in August and September followed by slow decrease, peacking in July and sudden decrease afterwards, and low density in summer followed by peaking in fall. Also the abundance in the kitchen and the room showed 3 types according to the level of density : "Symmetrical type" for high density, "Intermediate type" for medium density and "Relational type" for low density. During the investigation collected percents of adults and sex ratio were usually stable in the range of 35~40% and 0.30-0.40 respectively when densities were relatively high. Also percents of ootheca lay in a relatively stable range although it increased in November.

바퀴, Blattella germanica (L.),의 분포에 대한 광도효과 : 光度勾配處理를 한 사육상에서의 개체군 광선호성에 대한 관찰 Observations on Light Preferences of the Population in Rearing Cages Treated with a Grandient of Different Light Intensities

全胎秀,權太晟 부산대학교 1986 자연과학논문집 Vol.42 No.-

When german cockroaches were reared in a partitioned cage treated with a gradient of different light intensities, the population showed maximum preference at 8 and 15 lux at the low density, <8 lux at the medium density, and 60 and <8 lux at the high density. The light preference was more variable at the light acclimation of 100 lux than that of <10 and 1,000 lux. Dairy movement of the German cockroach among the compartments with different light intensites appeared to increase as the population density decreased. The aggregation of the population in the rearing cage was generally high at the medium density that at the low or high density. The effect of light acclimation on the aggregation was relatively low at the medium density. The intra-and inter- component aggregation among different ages were highest in young nymphs and lowest in old nymphs. The population density produced higher effects on the aggregation of nymphs than that of adults.

Tyrosine Hydroxylase in Japanese Medaka(Oryzias latipes) : cDNA Cloning and Molecular Monitoring of TH Gene Expression As a Biomarker cDNA 클로닝 및 생물지표로서의 TH 유전자 발현의 분자생물학적 추적

Shin, Sung-Woo,Kwon, Oh-Shin,Kim, Jung-Sang,Chon, Tae-Soo,Lee, Sung-Kyu,Koh, Sung-Cheol 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.4

최근 독성 유해물질의 환경으로의 방출로 인해 인간 및 생태계에 대한 위해성 문제가 심각하게 제기되고 있다. 독성화학물질을 포함한 여러 환경 오염물질의 위해성평가는 화학물질의 유해성과 노출량 측정을 동시에 측정함으로써 가능한데 이 경우 생물지표(biomarker)가 최근 각광을 받고 있다. 본 연구에서는 동물의 행동에 관련된 신경전달물질의 생성에 결정적 역할을 하는 tyrosine hydroxylase (TH) 및 그 유전자가 생물지표로서 이용 가능성이 있는지를 검토하였다. Ovary cDNA library의 PCR 스크리닝을 통한 송사리 TH 유전자를 부분적으로 클론하였으며(327bp), DNA 염기서열 분석 결과 쥐(rat)의 TH 유전자와 동일한 염기서열을 보였다. 그리고 다이아지논 처리구 및 무처리구에 송사리의 머리부분(head)및 몸통 부분(body)에서 추출된 총 RNA에 TH mRNA가 존재함을 RT-PCR를 통하여 확인하였다. 그러나 다이아지논의 처리효과가 송사리의 행동에 미치는 영향을 보기 위해서는 TH 의 발현을 보다 정량적으로 검토할 필요가 있을 것으로 판단된다. 생물지표로서 TH의 활성 및 mRNA의 기관별 또는 조직별 검출은 독성물질에 영향을 받는 어류 신경행동 변화를 모니터링 할 수 있는 유용한 수단이 될 것이다. 나아가 환경관리에 있어서 신경화학물질과 분자생물학적 상관관계를 통한 이상반응행동의 분석은 환경 위해성평가에 상당히 기여할 것이다. The release of hazardous waste materials into the environment poses serious risks in humans and ecoThe risk assessment of environmental polluincluding hazardous chemicals requires a commeasurement of hazard and exposure of the chemicals that can be achieved by toxicity evausing a biological system such as biomarkers. In this report we have tried to develop a biomarker used to elucidate a molecular basis of, and to monitor abnormal behaviors caused by diazinon in Japanese medaka (Oryzias latipes) as a model organism. First, an attempt was made to clone tyrosine hydroxylase gene from Japanese medaka that would be a candidate for a biomarker for neuronal modulations and behaviors. For monitoring experiments at behavioral and molecular biological levels, the fish were treated under different sublethal conditions of diazinon and their behavioral responses were observed. In this study we have successfully cloned a partial TH gene from the medaka fish through PCR screening of an ovary cDNA library. DNA sequencing analysis revealed that the amplified fragment was 327 by encoding 109 amino acids. Comparing the DNA sequence of medaka TH with other species, TH gene revealed the DNA sequence was completely identical to that of rat TH. In the RT-PCR, 330 by of mRNA was consistently amplified in all the treated samples including control. There were no significant differences in the TH expression level regardless of treating concentrations (1~5,000ppb) and time (0~48 hr). The reason appeared to be that RT-PCR was not performed using through a quantitative analysis normalized against an actin gene expression. Organ or tissue-specific detection of TH activity and mRNA as biomarkers will be a useful monitoring tool for neurobehavioral changes in fish influenced by toxic chemicals. Furthermore, quantitative analysis of locomotive patterns and its correlation with the neurochemical and molecular data would be highly useful in measuring toxicity and hazard of various environmental pollutants.