PUVA가 배양 각질형성세포의 성장과 배양 멜라닌세포의 성장 , 멜라닌양 , 수지상돌기 수 및 길이에 미치는 영향

전일선 ( Il Sun Jun ),박재경 ( Jae Kyung Park ),허충림 ( Choong Rim Haw ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.5

Background : The combination of psoralen and UVA (PUVA) as a model of photochemotherap, has been used in a wicle variety of cutaneous disorders such as psoriasis, vitiligo, mycosis fungoides and atopic dermatitis. The mechanism of PUVA of psoriasis is based on the fact. that PUVA causes photoconjugation of psoralens to DNA and a subsequent suppression of mitosis, DNA synthesis, and keratinocyte proliferation. Although PUVA apparently inhibits keratinocytie proliferation and is effective therefore in the treatment of psoriasis, PUVA increases pigmentations by stimulating melanocyte proliferation and melanin synthesis in vivo. Objective : We tried to investigate the PUVA effects on the proliferation and different,iation in cultured human keratinoc tes and melanocytes. Methods : We examined morphologic changes, and the number of the cultured human keratinocytes and melanoiytes and melanin contents in a control group and experimental group. (UVA group, 8-MOP group and PUVA group). i.e., UVA group was exposed to UVA at 60mJ, of. 8-MOP group was acJded at dose of 2 x 10 M to medium for 30 minutes. PUVA group was exposed to UVA at 60mJ, of after adding in 8 MOP at Zx 10 M for 30 minutes. Results : 1. Morphologic changes of cultured human keratinocytes and melanocytes. There were no significent changes between the control group and the experimental groups in keratinocytes and melanocytes after 24, 48 and 72 hours culture. The number and length of meianocyte dendrites showecl no significant differences between the groups after 24, 48 and 72 hours culture(p.>0.05). 2. Proliferation of cultured human keratinocytes and melanocytes 1) The number of keratinocytes in 8-MOP and PUVA groups decreased significantly more than in the control and LVA groups at 72nd hour after culture (p<0.01). 2) The number of melnocytes showed no differences between the groups at 72nd hour after culture (p>0.05). 3. Melanin contents in iultured human melanocytes The melanin contents increased significantly in the PUVA groups compared to that in the other groups at 72nd hour after culture (p<0.01). Conclusion : In culturel human keratinocytes, PUVA has no effect on the morphology and differentiation, hut inhibit proliferation. In cultured human melanocytes, PUVA has no effect on morphology and proliferation, but it increases the melanin contents. (Kor J Dermatol 1995;33(5): 886-894)

Boric Acid에 의한 알레르기성 접촉 피부염

전일선(Il Sun Jun),이미애(Mi Ae Lee),이무형(Mu Hyoung Lee),허충림(Choong Rim Haw) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.6

Boric acid(H BO) is a weak topical anti infective agent. It can cause acute borism due to the internal absorption. Although the frequency is very rare, boric acid can also induce contact dermat.itis after topical spplication. We present allergic contact dermatitis to boric acid. The patch test results showed a positive reaction to 2% boric acid solution. (Kor J Dermatol 1994; 32(6): 1099-1102)

과호산구 증후군

전일선(Il Sun Jun),이무형(Mu Hyoung Lee),허충림(Choong Rim Haw) 대한피부과학회 1995 대한피부과학회지 Vol.33 No.2

In 1968, Hardy and Anderson first described the term hypereosinophilic syndrome. This syndrome is characterized by persistent and prolonged eosinothila, with primarily hematologic, cardiac, neurologic and derrnatologic abnormalities. Cutaneous mmestations occur in 27-57% of patients. Two types of skin lesions have been noted: (1) erythrritous pruritic papules and nodules or (2) urticaria and angioedema. We report a case of hypereosinophilic syndrome with a vesicle eruption which is a rare skin lesion in tbis syndrome, in a 17 year old man. Diagnosis of hyprcsinophilic syndrome was established by clinical findings, rearked blood eosinophilia without a Brown cause, bone marrow aspiration and biopsy, liver scan and ultrasonography, and histopatholcgie findings of the skin. (Kor J Dermatol 1995; 33 (2): 332-338)

철분 결핍성 빈혈과 동반된 분절상 모발 색소이상증

전일선 ( Il Sun Jun ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.3

The peculiar heterochromasia of scalp hair characterizes by the irregular segmentation of black hair into alternating dark brown (dark) and white-to-silver (light) bands. A 17-year-old girl visited to our department because of abnormal discoloration of scalp hair and diffuse hair loss of 2 years' duration. But, the growth and color of her eyebrows, eyelashes, and pubic and axillary hair were normal. No abnormalities were noted in her nails and teeth. The size of hair shaft was 70um in diameter. The length of dark and light bands ranged from 5 to 35 mm and 7 to 20 mm, respectively. Hematologic tests showed iron-deficiency anemia, The results of other tests were all normal. Patient took oral iron with ferruglycine sulfate for 5 months. The light bands of hairs disappeared and the diameter of hair was thickened after treatment. We suggest that hair changes in this case were caused by iron-deficiency anemia.(Kor J Dermatol 1996;34(3): 430-433)

배양액에 따른 B16F1 생쥐 악성 흑색종 세포의 표현형의 변화

이무형 ( Mu Hyoung Lee ),전일선 ( Il Sun Jun ) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.5

Background : The influencion the environment on a culture is expressed via four routes. (1) the nature of the substrate or phase on or in which the cells grow (2) the physicochemical and physiological constitution of the medium, (3) the constitution of the gas phase, and (4) the incubation temperature. Melanization is closely related to the constitution and amounts of amino acids in the medium. Objectjve : The aim of this study is to investigate whether there are some differences of proliferation and melanization in cultured B,F, mouse melanoma cells according to different culture media. Methods : We examined the color of cell pellet, cell morphology, electron microscopic findings, cell counts and melanin conlensin BgF mouse melanoma cells cultured in Dulbeccos modified Eagles medium(DMEM), F-10, MCDB 153, Minimal essential medium(MEM), and RPMI 1640, respectively. Results : 1. The color of cell pellet., ringed from dark gray to light brown. The order of the darkness was DMEM, MEM, RPMI 1640, MCDB 153, and F-10 medium. 2. Most Bg, mouse melanoma cells had an epithelioid morphology, but a few cells in MCDB 153 medium showed dendrites. On the 4th day after culture, the cells in F-10 medium were larger than those in the other media. 3. In the electron microscopic. findings, BF, mouse melanoma cells in DMEM and MEM con tained numerous stage IV nelanosomes, however, those in RPMI 1640 and MCDB 153 medium contained a few, and those in F-10 medium did few. 4. The number of BF, mouse melanoma cells were 1.42 + 0.06 x 10, 1.42 + 0.12 x 10, l. 17 + 0.08 x 10, 0.73 0.06 x 10, 0.32 0.01 x 10, in RPMI 1640, DMEM, MEM, F 10, and MCDB 153 medium, respectively. 5. In the MTT assay, the order of the optical density of B,F, mouse melanoma cells in various media was as followings, DMEM, RPMI 1640, MEM, F-10, and MCDB 153. 6. Compared with the melanin contents of B;F, mouse melanoma cells in DMEM, they were 77.97% in MEM, 67.91% in RPMI 1640 and MCDB 153 medium, and 55.94% in F-10 medium. Conclusion : The phenotypic changes of BF, mouse melanoma cells were induced by various culture rnedia and were reversilvle. Since the phenotypes of cells can be changed by the culture media, researchers should choose the appropriate culture medium for the cells. (Kor J Dermatol 1995;33(5): 914-921)

Apocrine Gland Carcinoma

김낙인,전일선,하충림 대한피부과학회 1996 Annals of Dermatology Vol.8 No.1

Carcinomas of apocrine glands are rare and their diagnostic features are poorly defined. Only about 35 cases were reported in the literaturgs from 1911 to 19871. Their occurrence has been reported mainly in the axilla and breast area. We present a case of apocrine carcinoma arising from the left axilla. The literature is reviewed with respect to clinical and histopathologic findings.