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RISS 인기검색어
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한나영,전길자 ( Na Young Han,Gil Ja Jhon ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
The major component of glycolipids in antler was investigated by Folch-Suzuki partition method, Sephadex G-50 chromatography, DEAE-Sephadex A-25 chromatography, HPLC, and FAB-MS. Glycolipids, extracted in aqueous phase by chloroform-methanol-0.88% KCl aqueous solution, were analyzed on Sephadex G-50 column and most glycolipids containing band 1 were eluted in void volume because of the formation of micelles. The major fractions pooled from Sephadex G-50 column were eluted before gradient elution on DEAE-Sephadex A-25 column due to neutral property. Band 1 extracted from HPTLC plate was injected on HPLC column(μ-Bondapak NH₂ column) and four peaks were separated. The fractions containing major peak(3.17 min, 48.5%) were pooled and analyzed by negative-ion FAB-MS. As the result, it has been identified that the molecular weight of major component is 1058 and structural fragments is 98-98-142-98-142-98-142-98-142 or 98-240-240-240-240.
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한나영,전길자,Han, Na-Young,Jhon, Gil-Ja 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5
본 연구에서는 녹용중에 존재하는 gangliosides 성분을 조사하였다. Folch-Suzuki 분배법에 의해 수용액층으로 분배된 당지질 성분을 Sephadex G-50 column chromatography와 투석으로 정제한 후 용매추출법을 이용하여 ganglioside 성분만을 분리하였다. DEAE-Sephadex A-25 column chromatography와 HP-TLC로 분석한 결과 녹용의 70% 에탄올 추출액에는 GM3, GM4가 주요 ganglioside이었으며 GM1과 GM2가 소량 존재하였으나, chloroform/methanol 추출물에는 GM1이 주요 ganglioside이었다. 녹용 30g중에 $0.437{\mu}mol$의 ganglioside-bound sialic acid가 존재하였다. To determine contents of gangliosides in deer antler, we separated glycolipid from deer antler according to Folch-Suzuki partition method. The glycolipid was then purified by Sephadex G-50 column chromatography and dialysis. The gangliosides were purified from the separated glycolipid by solvent extraction, 2D-HPTLC, and DEAE-Sephadex A-25 chromatography. We found that GM3 and GM4 were major gangliosides and GM1 and GM2 were minor gangliosides in 70% ethanol extract from deer antler. However, GM1 was major ganglioside in chloroform/methanol extract from deer antler. The amount of ganglioside· bound sialic acid was $0.437{\mu}mol$/30g deer antler.
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한나영,전길자 ( Na Young Han,Gil Ja Jhon ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5
To determine contents of gangliosides in deer antler, we separated glycolipid from deer antler according to Folch-Suzuki partition method. The glycolipid was then purified by Sephadex G-50 column chromatography and dialysis. The gangliosides were purified from the separated glycolipid by solvent extraction, 2DHPTLC, and DEAF-Sephadex A-25 chromatography. We found that GM3 and GM4 were major gangliosides and GM1 and GM2 were minor gangliosides in 70% ethanol extract from deer antler. However, GM1 was major ganglioside in chloroform/methanol extract from deer antler. The amount of ganglioside-bound sialic acid was 0.437 μ㏖/30 g deer antler.
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한나영,전길자 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-
녹용의 생리활성 물질을 연구하기 위하여 당이 함유된 부분의 성분을 Folch-Suzuki분배방법,Sephadex G-50 chromatography, DEAE-Sephadex A-25 chromatography, High Performance Liquid Chromatograpy (HPLC)로 분리 정제하고 FAB-MS를 이용하여 구조를 분석하였다. 각 단계에서 당지질을 확인하는 방법으로 High Performance Thin Layer Chromatography(HPTLC)를 수행하였다. Chloroform-methanol-0.88% KCI수용액을 사용하여 분배된 수용액층을 Sephadex G-50 column에서 분리하였을 때, void volume에서 대부분의 당지질이 용출되는 것으로 보아 micelle을 형성하고 있음을 알 수 있었다. Sephadex G-50 column에서 용출되는 당지질을 DEAE-Sephadex A-25 chromatography결과 band 1(녹용에 존재하는 당지질중 주요성분)을 포함하는 대부분의 당지질이 농도 기울기를 걸기 전에서 용출되었다. 따라서 band1을 중성 당지질로 예측할 수 있었다. Band 1을 HPLC로 분석한 결과 4개의 peak로 분리되었다. 그 중 가장 많은 양으로 존재하며(48.5%), 3.17분 대에서 용출되는 부분만을 모아 negative-ion FAB-MS로 분석한 결과 이미지 물질은 분자량이 1058이고, 98-240-240-240-240Ehsms 98-98-142-98-142-98 -124-98-142의 분자구조를 가지는 것으로 확인할 수 있었다. The major component of glycolipids in antler was investigated by Folch-Suzuki partition method, Sephadex G-50 chromatography, DEAE-Sephadex A-25 chromatography, High Performance Liquid Chromatograpy HPLC, and FAB-MS. Glycolipids, extracted in aqueous phase by chloroform-methanol-0.88% KCI aqueous solution, were analyzed on Sephadex G-50 column and most glycolipids containing band1 were eluted in void volume because of the formation of micelles. The major fractions pooled from Sephadex G-50 column were eluted before gradient elution on DEAE-Sephadex A-25 column due to neutral property. Band 1 extracted from HPTLC plate was injected on HPLC column(μ-Bondapak NH_2 column)and four peaks were separated. The fractions containing major peak(3.17 min,48.5%)werepooled and analyzed by negative-ion FAB-MS. As the result, it has been identified that molecular weight of major component is 1058 and structural fragments is 98-98-142-98-142-98-142-98-142 or 98-240-240-240-240.
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