Bovine Leukemia Virus에 실험감염된 한국재래산양의 혈액상의 변동

이필돈 ( Pil Don Lee ),김종호 ( Jong Ho Kim ),정무형 ( Moon Hyung Jun ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.1

To elucidate pathogenesis of bovine leukemia virus(BLV) in Korean native goats, the goats experimentally infected with BLV were studied especially for the aspects of infectivity and hematological changes. The experimental goats were examined for 27 months by agar-gel immunodiffusion(AGID) test and syncytium formation assay. During this period, changes of total leucocyte, absolute lymphocyte and atypical lymphocyte were examined, and the distribution of surface immunoglobulin(sIg)-bearing cells and rosette forming cell(RFC) in the peripheral lymphocyte were also investigated. By indirect immunofluorescence(IFA) and complement dependent antibody cytotoxicity (CDAC) assay using monoclonal antibody(Mab) against bovine leukosis tumor-associated antigen(BL-TAA), changes of BL-TAA positive lymphocyte in peripheral blood were measured. The results obtained through the experiment were summarized as follows. 1. Antibody titers were measured by AGID using gP51 and P24 antigens. The animals were serologically converted at 2 months post-inoculation(pi) in gP51 antigen, whereas sero-converted at 4 months pi in P24 antigen. In comparison with antibody titers for gP51, P24 antigen showed lower titers throughout the trial period. 2. The peripheral lymphocytes from all of the infected goats, as co-cultivated with F81 cells manifested syncytial formation at 4 months pi. 3. On counting total leucocyte, lymphocyte and atypical lymphocyte, two out of four infected goats showed normal distribution, while No 2 of the remaining two revealed temporal and No 3, Persistant increasing number of the cells. 4. The optimal condition of rosette formation of the peripheral lymphocyte of normal Korean native goats was shown in the sheep erythrocyte treated with 0.1M AET for 30 mm at 37℃. When the lymphocytes were treated in nylon wool column, the number of sig-bearing cell were increased in the nylon wool adherent cells, but RFC was increased in the non-adherent cells. Of the infected goats, No 2 and No 3 showed significantly increasing number of slg-bearing cells at 18 months pi. 5. The lymphocytes of No 2 and No 3 goats reacted positively in IFA using Mab against BL-TAA at 12 months pi and 18 months pi, respectively. In CDAC test, all of four infected goats revealed positive reaction at 24 months pi. The higher positive rates were observed in No 2 and No 3 as compared with the remainders.

학술발표(學術發表) 연제(演題) 및 초록(抄錄) 제일부(第一部): 병리(病理),기생충(寄生蟲) 분야(分野) : 5. 대전광역시 애완견의 장내기생충 감염실태조사

오윤희,송운재,이필돈,박배근 한국가축위생학회 1999 학술대회논문집 Vol.1999 No.-

견정액(犬精液) 동결시(凍結時) seeding처리(處理)가 융해후(融解後) 정자(精子)의 활력(活力) 및 생존율(生存率)에 미치는 효과(效果)

김종호 ( Jong Ho Kim ),이필돈 ( Pil Don Lee ),유일정 ( Il Jeoung Yu ),김용준 ( Yong Jun Kim ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.3

To investigate effect of seeding on post-thaw motility and viability of canine spermatozoa, the semen from male dogs which had been proved to be fertile in the past were frozen and seeded during freezing process. Post-thaw motility and viability of canine sperm which were frozen and seeded were investigated according to different seeding temperatures of -5℃, -10℃, or -15℃ and also according to different concentration of glycerol of 2%, 5% and 10%. In addition, post-thaw motility of canine sperm frozen by direct freezing in a deep freezer or programmed freezing in a programmed cell freezer was investigated. Post-thaw motility of canine sperm was compared according to different seeding temperatures: The sperm seeded at -5℃ showed considerably higher post-thaw motility than that of non-seeding, and that seeded at -10℃ or -15℃, respectively, in 2% and 5% glycerol groups on both 2 and 7day after freezing(p<0.05). In 10% concentration of glycerol, the sperm seeded at each seeding temperature showed considerably higher post-thaw motility than that of non-seeding group on day 7 after freezing(p<0.01). Post-thaw viability of canine sperm was compared according to different seeding temperatures: The sperm seeded at -5℃ showed significantly higher post-thaw motility than that of non-seeding, and that seeded at -l0℃ or -15℃, in 5% and 10% glycerol groups on day 7 after freezing(p<0.05). In comparison of post-thaw motility of canine sperm seeded according to different concentration of glycerol, 5% glycerol group and 10% glycerol group showed considerably higher post-thaw motility than 2% glycerol group without difference between those two groups in all seeding temperatures (-5℃, -l0℃ and -15℃) on day 2 and 7 after freezing(p<0.01). In comparison of post-thaw viability of canine sperm seeded according to different concentration of glycerol, 5% glycerol group and 10% glycerol group showed the same considerably higher post-thaw viability than 2% glycerol group on each thawing day(p<0.01). The canine sperm frozen and seeded by programmed freezing method showed considerably higher post-thaw motility than that frozen by direct freezing method in all different seeding temperatures(-5℃, -10℃ and -15℃). These results indicated that the higher post-thaw motility and viability was obtained in the spermatozoa seeded than that of non-seeding, that among different seeding temperatures of -5℃, -l0℃ and -15℃, the sperm seeded at -5℃ showed higher post-thaw motility and viability than the other temperatures, also among different concentrations fof glycerol of 2%, 5% and 10%, the sperm frozen and seeded in 5% and 10% concentration of glycerol showed higher post-thaw motility and viability than that in 2% of glycerol, and that the sperm frozen and seeded by programmed freezing method showed higher motility than that by direct freezing method.

대전광역시 애완견의 장내기생충 감염실태조사

오윤희 ( Yoon Hee Oh ),송운재 ( Woun Jae Song ),이필돈 ( Pil Don Lee ),박배근 ( Bae Keun Park ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.4

In order to survey the prevalence of intestinal parasites in dogs, 304 fecal samples were taken from dogs in Taejon city. The prevalence and identification of intestinal parasites were determined by the fecal examinations using sheather`s floating technique and sedimentation methods and then Cryptosporidium oocysts were identified by kinyoun`s modified acid fast stainning method. The results were obtained as follows; 1. Parasite eggs and oocysts were detected in 105 samples(34.5%) from 304 cases of dog feces. 2. The 11 kinds of eggs and oocysts were isolated from the feces of dog. Those were Ancylostoma caninum(12.1%, 37 dogs), Trichuris vulpis(11.5%, 35 dogs), Toxocara canis(10.2%, 31 dogs), Isospora sp(7.2%, 22 dogs), Cryptosporidium sp(3.6%, 11 dogs), Toxascaris leonina(1.9%, 6 dogs), Strongyloides sp(1.9%, 6 dogs), Taenia sp(0.6%, 2 dogs), Diphylidium caninum(0.3%, 1 dog), Spirometra sp(0.3%, 1 dog) and Clonorchis sinensis(0.3%, 1 dog). 3. It was mixed infection such as single, double, triple and quadruple, 64.8%, 25.7%, 8.6% and 0.9%, respectively. 4. In indiviually-raised dogs, the infectious rate of T canis(11.4%), A caninum(13.2%), Cryptosporidium sp(6.1%), T leonina(2.6%) were higher than those of group raised dogs. But the infectious rate of T vulpis(12.1%) in group raised dogs was higher than that of individually-raised dogs. 5. Adults of Demodex and Sarcoptes which have been found in this survey are excluded in this report.

축종별 일본뇌염바이러스에 대한 항체보유율 조사

이상준 ( Sang Joon Lee ),정년기 ( Nyun Ki Chung ),송운재 ( Woun Jae Song ),장승익 ( Seung Ik Jang ),하숙희 ( Sook Hee Ha ),문병천 ( Byung Cheon Moon ),이필돈 ( Pil Don Lee ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.1

A total of 1,024 sera were collected from cattle(227), pigs(465), chickens(257) and dogs(75) raised or slaughtered in Daejeon metropolitan city from April to September 2002. Japanese enceplvlitis virus(JEV) antibodies in sera were detected by the haemagglutination inhibition test. The prevalence rates of JEV antibodies were 99.1%, 54.0%, 63.0% and 98.7% in cattle, pigs, chickens and dogs, respectively. In case of cattle and dogs, the monthly antibody-positive rates were as high as 85.7-100.0% and there were no differences among six months. In case of pigs, the monthly antibody-positive rate showed the lowest in April(6.4%) and the highest in July(100.0%) and it remained above 50% during the summer-time. In case of chickens, the monthly antibody-positive rate was 100.0% in July & August, 80.5% in June, 40.0% in May, 7.5% in September and 5.0% in April in order and there were distinct differences in seasons.

대전광역시 관내 단독주택지역내 어린이공원에서 채취한 토양중 기생충란 오염실태

정년기 ( Nyun Ki Chung ),오윤희 ( Yoon Hee Oh ),김수곤 ( Soo Kon Kim ),문병천 ( Byung Cheon Moon ),박희국 ( Hee Kuk Park ),이필돈 ( Pil Don Lee ),한인수 ( In Soo Han ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.3

In order to survey the prevalence of parasites in soil from playground in a detached dwelling area, 162 samples were taken from playground 54 unit. The survey was carried out during 3 months from July in 2000 to september in 2000. The results were obtained as follows: 1. Eggs of Toxocara canis were detected in 30 samples (18.5%) from 162 cases of playground. 2. The 3 kinds of eggs were isolated from the soil of playground. Those were Strongyloides spp 23.5%, Toxocara canis 18.5% and Trichuris vulpis 4.3%. 3. It was mixed infestation such as single (74.5%), double (23.7%) and triple(1.6%).