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이준범,Lee Byoung-hee,박정준,Hong Jeong In,Lee Dae-Weon 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
The tobacco whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a serious pest that transmits tomato yellow leaf curl virus (TYLCV) in tomato. The tobacco whitefly exhibits morphological similarity with the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) which also damages various plants in the green house. Therefore, it is very important to quickly and accurately diagnose whiteflies for applying pest manage ment strategies. In this study, we used a loop-mediated isothermal amplification (LAMP) assay to quickly and effectively detect B. tabaci. Primer sets were investigated for the specificity of B. tabaci by visible detection of the target DNA fragment. Two primer sets for pheromone biosynthesis activating neuropeptide (PBAN) gene were obtained from transcriptomic analysis of B. tabaci biotype Q. The PBAN-based LAMP primer set showed specific amplification of the target region. The optimal conditions for B. tabaci detection were 60 ◦ C for 60 min with four LAMP primers of PBAN. The minimum amount of genomic DNA required for visible detection was 100 ng. These results suggest that the PBAN-based LAMP assay can be applicable for field monitoring of B. tabaci.
Si 첨가 고탄소강의 인장 성질에 미치는 패턴팅 조건의 영향
이준범,이상윤 ( J . B . Lee,S . Y . Lee ) 한국열처리공학회 1993 熱處理工學會誌 Vol.6 No.2
Isothermal transformation behavior during patenting and variations of microstructure and tensile strength of patented wires were investigated in Si-added high carbon steel. The TTT curves of the steels were made for two different austenitizing temperature. As the salt bath temperature was increased, the observed microstructures were bainite at 450℃, the mixture of bainite and pearlite at 500℃, and to pearlite at 600℃, The tensile strength of patented wire exhibited the highest value when the structure was pearlite, while the bainitic structure showed the lowest.
이준범,Lee, Jun-Beom 대한석유협회 2002 석유와 에너지 Vol.2002 No.7
이 자료는 한국석유공사에서 발간하는 주간석유뉴스(2002. 5. 31)에서 발췌한 것임. -편집자 주-