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무(Raphanus sativus L.) 未熟胚 培養을 통한 培養條件이 體細胞 胚形成에 미치는 影響
김영칠,이용영 公州大學校 資源科學硏究所 1996 資源科學硏究論文集 Vol.4 No.-
These studied were carried out to understand factors affect to somatic embryogenesis through culture of immature embryo segment of radish. Results are summarized as follows: 1.Though compact calli were formed in MS media, there were not in 1/2MS media or 1/4 MS media.Direct embryogenesis was not detected in MS media but foundat in 1/2MS media. 2.Supplement of 2, 4-D 1 mg/L had an effect on induction of compact callus. 3.Some compact calli were induced from upper part segment of hypocotyl and lower part segment of cotyledon but not from lower part segment of hypocotyl. Somatic embryos were induced directly from lower part segment of cotyledon. 4.Pretreament of 35℃ for I day increased compact callus induction rate. 5.The frequencies of compact calli were formed from Taebaek variety were bigber than Kungjung. Compact callus from both varieties developed into embryos by subculture in growth regulators free media. 6.The frequency of comact callus was formed was 10% in dark condition but not in light condition. 7.Through subculture in growth regulators free media, 40% of compact callus developed into embryos but loose callus didn't.
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