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삽사리의 가계 분석에 사용할수 있는 DNA probe의 선정
이상로,이호섭,정운복,탁연빈,박희천,김종봉,하지홍 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1
DNA지문법은 범죄감식, 친자감별, 가계분석, twin zygosity의 결정, 집단 유전학, 행동 생태학 등의 분야(jeffreys et al., 1985:Gill et al., 1985, 1987 : Lander, 1989:Neufeld and Colman, 1990)에서 널리 활용될 뿐만 아니라 멸종위기에 처한 동식물의 육종과 보존을 위해서도 없어서는 안되는 중요한 연구방법이 되었다.(Georges et al., 1988:Burke et al., 1989). 최근 미국에서는 멸종위기에 처한 퓨마와 매의 보존을 위해 이들 동물들에 대한 RFLP연구를 행했는데 현존집단내 혈통관계에 대한 중요한 정보를 얻을 수 있었다
하지홍,정운복,이상로,탁연빈,김종봉 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1
The Sapsaree is a nearly extincted Korean native dog whose historic habitat has included much of the Southeastern Korea. The only existing population of Sapsaree consists of 50 dogs living in the small area of Kyung-San Goon in Kyung-Pook Province. Field observations of the population indicated the presence of two distinct morphological phenotypes that are divided into Blue- and Yellow-Sapsaree. A RFLP (Restriction Fragment Length Polymorphism) analysis using pV 47-2 probe and Sapsaree DNA indicated the existence of two distint genetic stocks concordant with the morphological phenotypes.
정운복,하지홍,김종봉,이상로,탁연빈 한국유전학회 1991 Genes & Genomics Vol.13 No.4
The Sapsaree is a nearly extincted Korean native dog whose historic habitat has included much of the Southeastern Korea. The only existing population of Sapsaree consists of 50 dogs living in the small area of Kyung-San Goon in Kyung-Pook Province. Field observations of the population indicated the presence of two distinct morphological phenotypes that are divided into Blue- and Yellow-Sapsaree. A RFLP (Restriction Fragment Length Polymorphism) analysis using pV47-2 probe and Sapsaree DNA indicated the existance of two distint genetic stocks concordant with the morphological phenotypes.
삽사리의 가계 분석에 사용할 수 있는 DNA probe 의 선정
정운복,하지홍,이호섭,김종봉,이상로,탁연빈,박희천 한국유전학회 1991 Genes & Genomics Vol.13 No.4
In order to breed the Korean native dog, sapsaree, which exist as a single population in KyungPook province, we are going to use DNA fingerprinting as a tool for studying the pedigree. In this experiment, 4 kinds of DNA probes, Exon 8, Intron B, M13, and pV47-2 were analysed by means of conventional Southern hybridization with Sapsaree genomic DNAs digested with restriction enzymes for their usefulness as probe. On the basis of their autoradiographic band patterns and resolution, pV47-2 was determined to be the best probe for studing the pedigree of sapsaree.