층화 3단계 무관질문모형

이기성,홍기학,손창균,Lee, Gi-Sung,Hong, Ki-Hak,Son, Chang-Kyoon 한국통계학회 2011 Communications for statistical applications and me Vol.18 No.4

본 논문에서는 사회적으로나 개인적으로 매우 민감한 조사에서 조사하고자 하는 모집단이 여러개의 층으로 구성되어 있는 경우에, 김종호등 (1992)이 제안한 2단계 무관질문모형에서 사용한 단순임의 추출법 대신에 층화추출법을 적용하여 각 층의 모비율에 대한 추정뿐만아니라 모집단 전체 모비율에 대한 추정을 할 수 있는 층화 2단계 무관질문모형을 제안하였다. 그리고 층화 2단계 무관질문모형을 층화 3단계 무관질문 모형으로 확장하였다. 또한, 제안한 2단계와 3단계 층화 무관질문모형들에 있어서 각 층의 표본배분에 대하여 비례배분과 최적 배분 문제를 고려하여 다루었다. 마지막으로 층화 2단계 무관질문모형과 층화 3단계 무관질문모형과의 상대효율을 비교하였으며, 그 결과 층화 3단계 무관질문모형이 층화 2단계 무관질문모형보다 효율성면에 있어서 더 우수함을 알 수 있었다. For procuring more sensitive information and estimating stratum target population proportion as well as an overall one form a sensitive population composed of several strata we suggest a two-stage stratified unrelated question model that uses stratified random sampling instead of simple random sampling in the two-stage unrelated question model by Kim et al. (1992) and extend it to the three-stage stratified unrelated question model. We also deal with the proportional and optimal allocation problems in each suggested model, compare the relative efficiency of the suggested two models, and show that the three-stage stratified unrelated question model is more efficient than the two-stage one in view of the variance.

A Conditional Indirect Survey Method

이기성,홍기학,손창권,남기성,Lee, Gi-Sung,Hong, Ki-Hak,Son, Chang-Kyoon,Nam, Ki-Seong The Korean Data and Information Science Society 2002 한국데이터정보과학회지 Vol.13 No.1

For improving the quality of survey dat a of sensitive character, we suggest a conditional in direct survey method. In th at method, only the respondents who answer directly to the less sensitive question respond indirectly to the more sensitive one by using the one sample unrelated question randomized response technique with the known $\pi_y$, the true proportion of unrelated group Y. We extend it to two sample method when $\pi_y$ is unknown. We also consider the case that people who possess less sensitive character answer untruthfully. Finally we compare our method with the methods of Greenberg et al. and Carr et al..

대청호 및 금강수역의 유기물 분해능과 오염의 신호지표

이기성,고동규,박영식,최청일,최영길 ( Ki Sung Lee,Dong Kyu Ko,Young Sik Park,Chung Il Choi,Yong Keel Choi ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.1

In order to test the microbiological degradability of organic compounds and self-clarification, cycle of phosphorus compound in Daechung Reservoir Lake and Kum River area, physico-chemical factors and various bacterial population were analyzed. Also exo-enzymes activities, the amount of chlorophyll, polyphosphate, and DNA amount of cells were measured during from April to September in 1993. As a result of physico-chemical factor analysis, self-clarification of Deachung Reservoir Lake on upstream of Kum River was recognized periodically. The community sizes of total heterotrophic bacteria were evenly distributed seasonally. However the community sizes of total heterotrophic bacteria in the middlestream and downstream were distributed higher than those in the upstream of Kum River. Also population densities of indicator microbes for the determination of pollution such as E. coli and antibiotics or heavy metal resistant bacteria were higher in Gapchun stream and the middlestream of Kum River than those in other areas. And the populations of the indicator microbes were tended to increase more during the summer than those in the other season. The various exo-enzymes were mainly contributed to the water environment, so the self-clarification of each sites may be proceeded gradually by the degradation of organic compounds. The amounts of poly-P were relatively high temporally before the blooming of phytoplankton. It was also high in the polluted area that the relative ratios of poly-P to DNA or to chlorophyll can be applied usefully for the biological index checking the unbalanced and eutrophicated state of nutrition in environment.

금강수역내에서 분리된 몇 종의 Ampicillin 내성균의 β-Lactamase 발현양상

이기성,고동규,최원창,문영길,박영식,최청일,최영길 ( Ki Sung Lee,Dong Kyu Ko,Won Chang Choi,Yong Keel Mun,Young Sik Park,Chung Il Choi,Yong Keel Choi ) 한국환경생물학회 1994 환경생물 : 환경생물학회지 Vol.12 No.1

In order to characterize the bacterial β-lactamase types occurred in Kum river area, this research was designed to analyze with identification upon ampicillin (Amp) resistant bacteria, and was investigated into relationships between β-lactamase expression (inducibility) and growth phase under ampicillin shift and with restriction patterns of R-plasmids. As the identification result upon 4 strains of Amp resistant bacteria, KL 601 is identified as Pasteurella maltocida, KL 602 as Enterobacter agglomerans, KL 609 as Pseudomonas maltophilia and KL 611 as Acinetobactor calcoaceticus, respectively. In order to investigate Amp effect upon growth, when the Amp resistant bacteria were cultivated in nutrient liquid LB medium, in LB medium added with Amp, or in LB medium in the manner of Amp shift, the changes in growth rate and lag phase could not be observed. However, upon the prototrophic strains, KL 602 and KL 609, examining Amp effect upon these two strains in minimal GM63 liquid media, even though there was no change of maximum growth yield, the latent lag phase become very lengthened. In nutrient medium, β-lactamase activities of these 4 strains were metabolically regulated in accordance with growth phase, on the other hand, in minimal medium were differently regulated in accordance with growth phase and with strains. It could be postulated that KL 609 strain has the M.W.24,000, β-lactamase type which E. coli comprise, however, that KL 601, KL 602 and KL 611 have the M.W.35,000, β-lactamase type which E. cloacae or C. freundii comprise. Through the whole growth phase, since Amp did not affect to phosholipid metabolism, there is no change in phospholipid compisition upon Amp effect. Main phospholipid are the phosphatidyl glycerol and cardiolipin in all 4 Amp resistant strains. So, it was very significant that there were no changes in phospholipid composition and in growth rate upon nutrient medium under Amp stress. In order to recognize whether Amp resistant strains comprise the plasmid or not, preparation of plasmids and restriction digestion with Bam H1 were carried out, KL 601 strain has about 30 Kb of plasmid, KL 609 and KL 611 strain have about 25 Kb of plasmid and KL 602 does not have the plasmid, however. Hence, it will be predicted strongly that β-lactamase gene in KL 602 might be encoded chromosomally. Moreover, considering the results upon the changes of β-lactamase acticity, upon β-lactamase inducibility and upon changes of periplasmic protein profile under Amp stress, its prediction is very recognizable.

층화 가법 양적속성 확률화응답모형

이기성,안승철,홍기학,손창균,Lee, Gi-Sung,Ahn, Seung-Chul,Hong, Ki-Hak,Son, Chang-Kyoon 한국통계학회 2014 응용통계연구 Vol.27 No.2

본 논문에서는 사회적으로나 개인적으로 매우 민감한 조사에서 조사하고자 하는 모집단이 여러 개의 층으로 구성되어 있고, 각 층이 양적인 속성으로 되어 있는 경우에 Himmelfarb-Edgell의 가법 모형과 Gjestvang-Singh의 가법 모형에 단순임의추출법 대신에 층화추출법을 적용한 층화 가법 양적속성 확률화응답모형을 제안하였다. 제안한 두 모형으로부터 각 층의 양적속성에 대한 모평균의 추정뿐만 아니라 모집단 전체 모평균에 대한 추정을 할 수 있는 이론적 체계를 마련하였다. 그리고 제안한 두 모형에서 비례배분과 최적배분 문제를 다루었으며, 각 배분법에 따른 분산식을 도출하였다. 마지막으로 두 층화 가법 양적속성 확률화응답모형들 간의 효율성을 비교해 본 결과 Gjestvang-Singh의 층화 가법 모형이 Himmelfarb-Edgell의 층화 가법 모형보다 효율적으로 나타났고, 특히 hh값이 작을수록 즉, 제시한 모형의 특성이 직접질문에 가까워질수록 Gjestvang-Singh의 층화 가법 모형의 효율성이 커짐을 알 수 있었다. For a sensitive survey in which the population is composed by several strata with quantitative attributes, we present an additive stratified quantitative attribute randomized response model which applied stratified random sampling instead of simple random sampling to the models of Himmelfarb-Edgell's additive quantitative attribute model and Gjestvang-Singh's. We also establish theoretical grounds to estimate the stratum mean of sensitive quantitative attributes as well as the over all mean. We deal with the proportional and optimal allocation problems in each suggested model and compare the relative efficiency of the suggested two models; subsequently, Himmelfarb-Edgell's model is more efficient than Gjestvang-Singh's model under the condition of stratified random sampling.

Rhizopus oryzae 의 카드뮴 해독기작과 이에 관련된 동위효소의 변화 양상

이기성,김영호,박영식,박용근 ( Ki Sung Lee,Young Ho Kim,Young Sik Park,Yong Keun Park ) 한국균학회 1995 韓國菌學會誌 Vol.23 No.1

Sccharomyces uvarum의 protoplast 형성 및 intact cell과 protoplast의 phosphatase 활성도 비교

이기성,김영호,Lee, Ki-Sung,Kim, Young-Ho 배재대학교 자연과학연구소 1999 自然科學論文集 Vol.11 No.1

효모세포의 원형질체 최적형성을 위한 stabilizer의 종류 및 농도, pH 그리고 lysis 방법을 조사하는 한편, intact cell과 protoplast사이의 효소활성도 및 poly-P 생합성율을 비교하였다. 그 결과 protoplast 형성에 있어 snail gut enzyme은 5시간, drisielase는 3시간 정도의 incubation 시간이 필요했으며, stabilizer로는 0.8 M mannitol, 6 M KCl이 좋았다. Protoplast는 intact cell에 비해 ALPase 활성은 22-27%, ACPase는 4-15% 정도 감소하였으며, poly-P 형성은 protoplast에서 유의한 증가가 일어나지 않았다. In this study, the stabilizer, PH and lysis method for optimum condition of S. uvarum protoplast formation were investigated, and also enzyme activity and poly-P formation of intact cell and protoplast mere compared. Upon protoplast formation, incubation time of 5 hours in snail gut enzyme and 3 hours in drisielase were reignited. 0.8 Mole mannitol and 6 mole KCl were apt to protoplast formation. Protoplast was contained less 22-27 percentage in ALPase, 4-15 percentage in ACPase than intact cell. Accumulation of inorganic polyphosphate did not increase significently in protoplast compared with intact cell.

Rhizopus oryzae 의 생리 생화학적 카드뮴 해독기작

이기성,김영호,박영식,박용근 ( Ki Sung Lee,Young Ho Kim,Young Sik Park,Yong Keun Park ) 한국균학회 1995 韓國菌學會誌 Vol.23 No.1

금강수역내 항생제 및 중금속내성균의 분포 및 동시내성빈도

이기성,고동규,최원창,오태엽,박영식,최청일,최영길 ( Ki Sung Lee,Dong Kyu Ko,Won Chang Choi,Tae Youp Oh,Young Sik Park,Chung Il Choi,Yong Keel Choi ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.2

As the basic study to solve the recent big problems upon the bacterial multi-drug resistance recognizing seriously from the viewpoint of microbial ecology, clinical medicine and public health, this study was carried out temporally and spatially upon Kum river area that has been recently accelerated the pollution for the purpose of analysis such as distribution of heterotrophic bacteria, distribution of resistant bacteria upon prevalently utilizing antibiotics(Ampicillin, Amp ; Chloramphenicol, Cm ; Tetracycline, Tc ; Kanamycin, Km ; Streptomycin, Sm) and heavy metal(mercury, Hg ; cadmium, Cd), frequency of multi-drug resistances, frequency of resistant factor transfer, compatibility and/or incompatibility. In May, 1992, community sizes of total heterotrophic bacteria reached to 5×10 exp (7)CFU/㎖ in site 1(1S, Kapchun stream`s water), 5×10 exp (8)CFU/㎖ in site 2(2S, Kapchun soil) where industrial and domestic sewages were chiefly flowed, and reached to 1×10 exp (8)CFU/㎖ in site 3(3S, Sanne stream`s water), 1×10 exp (7)CFU/㎖ in site 4(4S, Sanne soil) where agricultural domestic sewages were. Total heterotrophic bacteria in Kapchun area(1S, 2S) were distributed 50 times higher than those in Sanne area(3S, 4S) respectively. Compared with water, soil contained 10 times higher in heterotrophic bacteria. Of heterotrophic bacteria, compositional ratios of resistant bacteria upon antibiotics and heavy metals were shown to the level of 0.07% in May, 1992 and to the level of 0.2% in October, 1992. So, frequency of resistant bacteria increased in October about 3 times higher than in May. The frequent order of resistant factor was Tc, Km, Cd, Amp, Hg, Cm, in May, 1992 and was Cd, Amp, Sm, Hg, Km, Tc, Cm, in October, 1992, respectively. Especially, Cd resistant bacteria were distributed upon all experimental sites more frequently in October, 1992 than in May, 1992. Passing through the rainy period in summer, the distributional change of resistant bacteria indicate strong evidence for the influx of large amount of sewage containing lots of resistant bacteria and of pollutants to the Kum river area. Considering the genetic linkage between antibiotics resistance and heavy metal resistance and distributional change of multi-drug resistant bacteria, resistant factor such as Tc, Amp, Cd were shown the highest frequency of simultaneous coexistence, so they are closely linked with other resistant factors the most from the viewpoint of molecular genetics. Most of resistant bacteria comprise generally simultaneous multi-drug resistance upon 5 to 3 drugs. As especial results, considering the relationship of simultaneous coexistence in antibiotics and heavy metal resistance, Cd resistance bacteria comprise other resistance factors such as Tc(70%) and Amp(68%) simultaneously and frequently and Hg resistant bacteria, such as Tc(80%) and with Cd(70%). In the transferability and incompatibility upon resistant factors, only 1% of resistant factors was the conjugative, transferable type plasmid, and 99% of them showed the incompatibility in the other strains.

Pseudomonas sp. strain #A1에서 C-P 화합물 분해 유전자의 Cloning

이기성,조홍범,김수기,Lee, Ki-Sung,Cho, Hong-Bum,Kim, Soo-Ki 배재대학교 자연과학연구소 1999 自然科學論文集 Vol.11 No.1

C-P 화합물(Pn; phosphonate)의 일종인 glyphosate(GPS)를 인산원으로 이용하는 Pseudomonas sp. strain #A1으로부터 GPS 분해 유전자 및 2-aminoethylphosphonate(AEPn), methyl-phosphonate(MPn)와 같은 Pn의 분해 유전자를 클로닝하였다. Mini-Mu plasmid를 이용한 in vivo molecular cloning 결과 약 10-19 Kb의 $AEPn^+$ clones, 10 Kb의 $MPn^+$ clones, 12-18 Kb의 $GPS^+$ clone들을 얻었으며 E. coli의 $\Delta phn$ mutants에 transformation 하였을 때 각각의 Pn에 대한 다양한 phenotype을 나타냈다. 따라서 Pseudomonas sp. strain #A1에서는 적어도 3종류의 Pn 분해대사 경로를 갖고 있는 것으로 예측된다. 뿐만 아니라, 각각의 phn clone($AEPn^+$, $MPn^+$, $GPS^+$)들은 PHO regulon의 조절유전자 phoBR에 의존하여 발현하였다. C-P compounds(Pn; phosphonate) such as glyphosate(GPS), aminoethylphosphonate(AEPn) and methyl-phosphonate(MPn) biodegrading genes were cloned from Pseudomonas sp. strain #A1 Which assimilated GPS as sole phosphorous source. Carrying out the in vivo molecular cloning by means of Mini-Mu plasmid, the size of clones($AEPn^+$, $MPn^+$, $GPS^+$) for the gene to degrade C=P compounds are 10-19Kb, 10Kb, and 12-18 Kb, respectively. Moreover, they expressed the phenotype for each Pn when they were transformed into $\Delta phn$ mutants. Hence, it is postulated that Pseudomonas sp.#A1 has three kind of Pn degradative pathway, separately. The phn clones($AEPn^+$, $MPn^+$, $GPS^+$) are verified as the members of PHO regulon because of their phoBR-dependent characteristics.