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- 저자 : 윤웅찬 ( Young Eun Kim (검색결과 3 건)
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동물 경조직 단백성분의 조성과 생리기능에 관한 연구 녹각의 경단백질에 대하여
김영은,이승기,윤웅찬 ( Young Eun Kim,Seung Ki Lee,Ung Chan Yoon ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.1
With an attempt to elucidate the biologically functioning groups of deer horn, we obtained, in this investigation, a protein fraction after extraction of the pulverized deer horn with 5M-urea followed by decalcification with acetic acid. The amino acid composition of deer horn extracted came up to 16 kinds. And analysis of the protein extracted with 5M-urea gave values, as per cent, glycine, 24.51 % ; proline, 11.09% ; glutamic acid, 10.26%, which come up to 46. 86% of the; total amino acids initially present. The existence of glucose and galactose is confirmed with the results of thin-layer chromatography being carried out many times. And the total amounts o_f hexose and hexosamine are accounting for 0.74% and 0.87% for each. The present communication described as above has affirmed the protein from deer horn to be a sort of collagen. However, it can be focused on the significance of the existence of cystine and the absence of valine in deer horn. The contents of inorganic elements detected with emission spectroscophy are listed as following: total inorganic elements are detected to be 13 kinds. And the ash-contents of deer horns are 55.6% (w/w). The quantitative analysis for the ash shows that it contains 36.79 of Ca and 1.42% of Mg against ash contents by weight per weight.
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동물 경조직 단백성분의 조성과 생리기능에 관한 연구 녹각의 경단백질에 대하여
김영은,이승기,윤웅찬,Kim, Young-Eun,Lee, Seung-Ki,Yoon, Ung-Chan 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.1
동물 경조직 단백성분의 연구의 일환으로 녹각 및 녹용의 생화학적 기능을 나타내는 성분을 밝히기 위하여 녹각 분말을 0.5M 초산으로 탈칼슘을 한 후 5M 요소로 단백질부분을 추출하였다. 추출한 녹각단액의 아미노산 구성은 16종류에 달했으며 이중 glycine은 24.51%; proline 11.09%; glutamic acid는 10.26%가 되어 검출된 전 아미노산의 45.86%를 차지하고 있음이 밝혀졌다. 박충크로마토그라피로 glucose와 galactose 및 hexoamine의 존재를 확인하였고 정량분석 결과 총 hexose의 양은 추출단백의 0.74%이고, 총 hexoamine의 양은 0.87%였다. 본보에서 보고한 바와 같은 아미노산의 구성비율로 미루어 녹각단백은 일종의 collagen으로 추정하였다. 그러나 녹각단백중에 존재하는 아미노산 구성중 특기할 점은 cystine이 존재하는 반면 valine이 검출되지 않았다는 점이다. emission spectroscopy로 검출된 무기원소의 종류는 13종이며 회화잔사의 함량은 녹각무게의 55.6% (w/w)이며 이 회화잔사중 Ca은 회화잔사의 36.79%이고 Mg은 1.42%를 차지하고 있음을 청량분석의 결과 규명하였다. With an attempt to elucidate the biologically functioning groups of deer horn, we obtained, in this investigation, a protein fraction after extraction of the pulverized deer horn with 5M-urea followed by decalcification with acetic acid. The amino acid composition of deer horn extracted came up to 16 kinds. And analysis of the protein extracted with 5M-urea gave values, as per cent, glycine, 24.51%; proline, 11.09%; glutamic acid, 10.26%, which come up to 46.86% of the total amino acids initially present. The existence of glucose and galactose is confirmed with the results of thin-layer chromatography being carried out many times. And the total amounts of hexose and hexosamine are accounting for 0.74% and 0.87% for each. The present communication described as above has affirmed the protein from deer horn to be a sort of collagen. However, it can be focused on the significance of the existence of cystine and the absence of valine in deer horn. The contents of inorganic elements detected with emission spectroscophy are listed as following: total inorganic elements are detected to be 13 kinds. And the ash-contents of deer horns are 55.6% (w/w). The quantitative analysis for the ash shows that it contains 36.79% of Ca and 1.42% of Mg against ash contents by weight per weight.
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녹용의 약효 성분에 관한 연구 ( 1 ) 녹용 , 녹각 , 고래 코 연골 , 상어 척수 연골의 화학조성에 관하여
김영은,이승기,윤웅찬,김정숙 ( Young Eun Kim,Seung Ki Lee,Ung Chan Yoon,Jung Sook Kim ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.2
In this study, an attempt has been made to investigate the differences between the chemical components of antler (Corpus Cervi Parvum) and old antler (Cornus Cervi) . Antler was cut from Cervus nippon taiouanus (3 years and 8 months old) and directly fronzen to storage in solid carbon dioxide. The antler was separated into velvet layer ancf spongy bone layer and cut into small pieces. After dehydration and removal of lipids from the antler with acetone, chloroform-methanol (1 : 1) solvent system, qualitative and quantitative analyses were carried out to compare their chemical components in tissue level with those of old antler spongybone layer, shark backbone cartilage and whale nasal cartilage. Quantitative analyses of hexose, pentose, hexosamine, uronic acid, sialic acid, ester-sulfate, hydroxyproline, total nitrogen and inorganic contents were carried out respectively for antler velvet layer, antler spongybone layer, old antler spongybone layer, shark backbone cartilage and whale nasal cartilage. And their relative amounts of the chemical components to the corresponding dry weight of samples were listed in Table XII. The neutral sugar compositions of samples were identified by gas-liquid chromatography. Antler velvet layer, old antler spongybone layer and whale nasal cartilage contained same kinds of neutral sugars, such as glucose, galactose, mannose, fucose, deoxyribose, ribose, arabinose and xylose. But antler spongybone layer and shark backbone cartilage did not show any sign of existence of fucose though they contained the same kinds of neutral sugars as the above. The gas liquid chromatography of aminosugars was also carried out. The results said that all five animal tissues contained both glucosamine and galactosamine. From the results of analytical data, the presence of mucopolysaccharides in antler and old antler was revealed. And the contents of hydroxyproline and total nitrogen of samples suggested that the antler-protein was mainly composed of collagen. The relative amounts of hydroxyproline to corresponding dry weight of samples showed that antler velvet layer exhibited the highest amounts (9.09%) and shark backbone cartilage (3.50%) exhibited the lowest amounts of hydroxyproline. The mucopolysaccharide of whale nasal cartilage was known mainly as chondroitin sulfate A and that of shark backbone cartilage mainly as chondroitin sulfate C. A further study on the mucopolysaccharide-protein complex of antler and of old antler is being carried out and will be presented in the next report.
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