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남정훈(Jeong Hun Nam),박종철(Jong Chul Park),유상배(Sang Bae Yu),정용일(Yong Il Chung),태기융(Gi Yoong Tae),김정주(Jung Ju Kim),박용두(Yong Doo Park),장정원(Jeong Won Jahng),이종호(Jong Ho Lee) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.3
As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase(MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel-Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.
하이아루론산을 기재로 한 하이드로젤-나노입자 복합체와 rhBMP-2를 이용한 골재생
이종호 ( Jong Ho Lee ),김성민 ( Soung Min Kim ),박종철 ( Jong Chul Park ),성미애 ( Mi Ae Sung ),유상배 ( Sang Bae Yu ),남정훈 ( Jeong Hun Nam ),정용일 ( Yong Il Chung ),김정주 ( Jung Ju Kim ),박용두 ( Yong Doo Park ),태기융 ( Gi Y 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
As an efficient controlled release system of rhBMP-2, a functional nanoparticle-130 KDa 5% Hyaluronic Acid (HA) based hydrogel-Nanoparticle(NP) complex was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase (ALP)/Osteocalcin activity and mineral contents analysis, based on the rat calvarial critical size defect (8 mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded HA based hydrogel-NP complex and the BMP-2 loaded HA based hydrogel as compared to only HA hydrogel group with the period of 4 weeks. These improved results included areas such as radiodensity, the bone specific ALP activity, the osteocalcin immunoreactivity, and the ratio of calcium and phosphate contents with respect to normal bone in the regenerated bone area. Although superiority of bone regenration in the rhBMP-2 loaded HA based hydrogel-NP complex group could not be confirmed statistically, all the evaluation parameters were higher in the rhBMP-2 loaded HA based hydrogel-NP complex group than in the the BMP-2 loaded HA based hydrogel group. These results indicate that the HA based hydrogel with nanoparticle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced BMP-2 scaffold.