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맥아당결합 단백질에 융합된 면역결핍 바이러스 인테그라제의 생산 및 분석
김도진(Do Jin Kim),오유택(You Take Oh),신차균(Cha Gyun Shin) 대한약학회 1998 약학회지 Vol.42 No.1
Retroviral integrase is required for integration of viral DNA into the host cell chromosome. Human immunodeficiency virus type-1 integrase was partially purified as a part of a fusion protein linked to a maltose-binding protein and characterized in terms of an endonucleolytic activity. The concentration of the fusion protein purified through an amylose column was about 12mg/ml. Indicating that the solubility of the fusion protein is highly increased by the presence of a maltose-binding protein, considering that the integrase protein alone is poorly solubilized. The endonucleolytic activity of the fusion protein was detected at 0.1 to 1.OmM Mn++ ion, but not at any concentrations tested of Mg++ ion.