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3D 형태의 PLGA 지지체에서 뷰틸레이트하이드록시애니솔의 방출거동 및 골수간엽줄기세포의 신경세포화
오아영 ( A Young Oh ),김순희 ( Soon Hee Kim ),모종현 ( Jong Hyun Moh ),김근아 ( Geun Ah Kim ),이종문 ( John M. Rhee ),이해방 ( Hai Bang Lee ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.2
Bone marrow stromal cells(BMSCs) can be differentiated into chondrocytes, osteocytes, neuronal cells under the controlling of culture condition. We fabricated butylated hydorxyanisole(BHA) loaded poly(L-lactide-coglycolide) (PLGA) scaffolds(BHA/PLGA) for neurogenesis of BMSCs and to observe the release behavior of BHA. BHA/PLGA scaffold were manufactured as the concentrations of 200, 400, and 600 μM of BHA by solvent casting/ salt leaching method. We isolated and cultured BMSCs from the tibias of adult female Fischer rat, we seeded BMSCs into the inner core of the hybrid scaffold. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazol- 2yl)-2,5-diphenyl-2H- tetrazolium bromide(MTT) test. Morphology of celluar adhesion were confirmed by scanning electron microscope SEM). The effect of BHA/PLGA scaffold on the neural differentiation of rat BMSCs were assessed RT-PCR. According to results, aspect of release of BHA in BHA/PLGA matrix showed that the initial burst of BHA was lower in scaffold than film and released continuously during the desired period in delivery system. BHA 600 μM loaded scaffold revealed low cell viability by MTT assay results. Mophology of BMSCs changed like a neural cell in 200 and 400 μM BHA/PLGA by SEM observation. In addition, 200 and 400 μM BHA/ PLGA scaffold showed higher expression of neural markers. We conclude that neural differentiation of BMSCs in BHA/PLGA scaffold could be possible for long period.
증례보고 : 소아에서 전신 마취 후 발생한 일과성 악하선 종창
박희연 ( Hee Yeon Park ),오아영 ( Ah Young Oh ),윤혜정 ( Hye Jeong Yun ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.53 No.4
Acute transient salivary gland swelling is a rare complication of general anesthesia. These swellings usually resolve over a few hours without sequelae. The etiology of the salivary gland swelling is unclear. However, they are considered to be associated with cough or straining on the endotracheal tube and mechanical blockage of salivary gland duct. Anesthesiologists should know the possible causes, lack of clinical significance, and the course of these transient salivary gland swellings in order to reassure the patient. We report bilateral submandibular gland swelling developed after endotracheal anesthesia in a 4-year-old girl. (Korean J Anesthesiol 2007; 53: 532∼4)
증례보고 : 상부기관협착증을 가진 환자의 기관절제와 단단문합술에서의 후두마스크를 사용한 마취관리
권태명 ( Tae Myoung Kwon ),윤미자 ( Mi Ja Yun ),강종만 ( Jong Man Kang ),오아영 ( Ah Young Oh ) 대한마취과학회 2006 Korean Journal of Anesthesiology Vol.51 No.4
Tracheotomy and end-to-end anastomosis is a relatively rare operation, but it is one of the most challenging for anesthesiologists. During surgery, the principal anesthetic consideration is to maintain the ventilation and oxygenation throughout the procedure. We experienced anesthetic management of a 49-year-old man with upper tracheal stenosis after long-term intubation and ventilation care. The stenotic lesion was located at 2 cm below the vocal cords, and the length of the stenotic segment was about 2 cm. The anesthesia was induced and maintained with intravenous propofol and alfentanil. The airway was managed with LMA and distal tracheal intubation on the surgical field and we were able to maintain adequate ventilation and oxygenation throughout the operational period. (Korean J Anesthesiol 2006; 51: 499~503)
김수미 ( Su Mi Kim ),김순희 ( Soon Hee Kim ),박상욱 ( Sang Wook Park ),오아영 ( A Young Oh ),김근아 ( Geun Ah Kim,),이종문 ( John M. Rhee ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.1
Olfactory ensheathing cells(OECs) transplantation is a promising orpotential therapy for spinal cord injury(SCI). Bone marrow stromal cells(BMSCs) contain mesenchymal stem cells and progenitor cells. BMSCs can differentiate into various cell types, including muscle and brain cells. In this study, we examined the feasibility of using BMSCs as a source of stem cells for the differentiation into OECs by co-culture approach. Only OECs and BMSCs were cultured as control group. The experimental group designed that OECs were transferred on transwell and insert this OECs culturing were co-cultured with BMSCs attached on 6 well bottom. Another experimental group cultured BMSCs in OEC-derived conditioned medium. Cultured cells were fixed at co-culture day 3, 7, 14 and 21. The expression of GFAP and P75 were characterized by immunocytochemistry. RT-PCR was used to evaluate the gene expression of P75, NSE and NF in BMSCs co-cultured with OECs. Our results showed that BMSCs able to differentiate into OEC-like cells using co-culture system in vitro. These findings could be helpful for the development of the cell-based therapeutic strategies for CNS repair.
PLGA/DBP지지체에 파종된 신경계세포가 골수간엽줄기세포의 분화와 증식에 미치는 영향
김수미 ( Su Mi Kim ),김순희 ( Soon Hee Kim ),김초민 ( Cho Min Kim ),오아영 ( A Young Oh ),김근아 ( Geun Ah Kim ),이일우 ( Il Woo Lee ),이종문 ( John M. Rhee ),강길선 ( Gilson Khang ),김문석 ( Moon Suk Kim ),이해방 ( Hai Bang Lee ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4
Poly(lactic-co-glycolic acid)(PLGA) is a biodegradable synthetic polymer with acceptable mechanical strength and has well-controlled degradation rate. Demineralized bone particle(DBP) contains powerful bioactive molecules. Bone marrow mesenchymal stem cells(BMSCs) are important progenitor and supporting cells that have the intrinsic ability to self-renewal and differentiate into multiple functional cells. In this study, we fabricated natural/ synthetic hybrid PLGA scaffolds included 20% of DBP(PLGA/DBP). Schwann cells(SCs) and olfactory ensheathing cells(OECs) were seeded on PLGA/DBP scaffolds. We confirmed that effects of PLGA/DBP scaffolds seeded OEC and SC on proliferation and differentiation of BMSCs. PLGA/DBP scaffolds seeded OECs and SCs were superior to differentiation into neuronal cell proliferation of BMSCs. This phenomenon was expected due to cytokine secreted by OECs and SCs promote proliferation and differentiation of BMSCs.