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기관 특이적인 Antisense 를 이용한 콩과식물의 질소고정 유전자의 연구
이제성,양문식 ( Je Sung Lee,Moon Sik Yang ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
Nodulins are organ-specific plant proteins induced during symbiotic nitrogen fixation in legume plants. Nodulins play both metabolic and structural roles within infected and uninfected nodule cells. Leghemoglobin, the most abundant nodulin, gene and chloramphenicol acetyl transferase (CAT) gene as a reporter were used to demonstrate that antisense regulation system worked in organ-specific manner in transformed and regenerated Lotus. CAT activity was knocked out in only nodules when Lotus corniculatus transformed with CAT gene was retransformed with antisense-CAT gene under the control of leghemoglobin promoter. This system can be used to study the functions of nodulin genes and to develop plants with new traits.