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가시오갈피나무 부정근(不定根) 배양(培養)에서 부정근(不定根)의 생장(生長)과 Eleutheroside유(類)의 생산(生産)에 미치는 Salicylic Acid 처리(處理)의 영향
안진권 ( Jin Kwon Ahn ),이위영 ( Wi Young Lee ),박응준 ( Eung Jun Park ) 한국임학회 2011 한국산림과학회지 Vol.100 No.2
생물반응기를 이용한 가시오갈피 부정근 배양시에 salicylic acid를 농도별(0, 5, 10, 20, 40, 80 μM)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Salicylic acid 처리농도별 부정근의 생장은 무처리구에서 4.4 g dry weight(DW)/L로 가장 높았으며, salicylic acid 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B, E 및 E1의 함량은 대체로 salicylic acid 농도가 높을수록 증가하여 80 μM 처리구에서 각각 179.5, 1169.9 및 45.4 μg/g DW로 생산량이 가장 많았다. 배지 1 L당 eleutheroside류의 총생산량은 무처리구에서 4975.8 μg/L를 생산하여 가장 우수하였다. 80 μM의 salicylic acid 처리 후 15일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 salicylic acid처리 후 9일째, eleutheroside E 및 E1은 처리 후 6일째 각각 가장 많은 생산량을 보여주었다. This study was carried out to investigate the dose-dependent effect of salicylic acid on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus. The highest biomass production (5.4 g DW/L) was observed in the absence of salicylic acid, while the root growth was significantly decreased by increasing the concentration of salicylic acid. Salicylic acid stimulated the production of both eleutheroside B, E and E1. The highest levels of eleutheroside B (179.5 μg/g DW), E (1169.9 μg/g DW) and E1 (45.4 μg/g DW) were obtained by the addition of 80 μM of salicylic acid. The maximum eleutheroside production was 4975.8 μg/L when salicylic acid was not added. In addition, when the adventitious roots were cultured in the basal medium supplemented with 80 μM of salicylic acid, the highest levels of eleutheroside B was observed at the 9th day, while eleutheroside E and E1 were observed at the 6th day, respectively.
전나무 수형목의 풍매차대에 (風媒次代) 대한 생장과 재질특성의 유전력
안진권 ( Jin Kwon Ahn ),이위영 ( Wi Young Lee ),조동광 ( Dong Gwang Jo ),손두식 ( Doo Sik Son ) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.4
Growth and wood quality characters such as height, DBH, specific gravity, traceried length and width were measured for 16-year-old open-pollinated progenies of 38 selected plus trees of Abies holopleylla Max. The results were as follows : 1) The superior families in growth and wood quality characters were Chonbuk 4(498.2㎝) and Kyonggi 9(485.7㎝) for height, Kyonggi 12(9.2㎝) and 9 (9.1㎝) for DBH, Kaongbuk 2 (0.480) and Kangwon 7(0.473) for specific gravity, Kyonggi 9(2.77mm) and Kangwon 11 (2.71mm) for tracheid length and Kyonggi 9(0.039mm), Kangwon 11, 12 and Kyunggi 7(0.036mm) for tracheid width, respectively. 2) There were significant differences among the families in height and specific gravity at 1% and 5% level. 3) There were high significant negative correlations between specific gravity and other characters such as height, DBH, tracheid length and width at 1% level(r=-0.242^(**)-0.635^(**)). 4) The heritabilities were 0.55 for height, 0.25 for DBH, 0.44 for specific gravity, and 0.24 for tracheid width.
생물반응기 배양에서 생장조절제에 따른 섬오갈피 부정근 증식 및 Eleutheroside와 Chlorogenic Acid 생산
안진권,박소영,이위영,이정주,Ahn Jin-Kwon,Park So-Young,Lee Wi-Young,Lee Jeong-Ju 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.1
생물반응기를 이용한 섬오갈피 부정근 배양시 첨가되는 생장조절제의 종류와 농도에 따른 부정근의 생장과 이차대사산물 생산과의 관계를 조사하였다. IBA 농도별 부정근 생장은 IBA $2.0\;\cal{mg/L}$에서 6.7 g DW/L로 가장 높았으나, eleutheroside E 함량은 IBA 농도에 따라 차이가 없었다 TDZ 농도에 따른 부정근 생장은 농도가 증가함에 따라 생장도 증가하여 eleutheroside E 함량은 IBA $3.0\;\cal{mg/L}$에 TDZ $0.1\;\cal{mg/L}$ 처리구에서 $3,327\;{\mu}g/g$ DW로 가장 생산량이 많았다. 이와 달리 eleutheroside B ($153\;{\mu}g/g$ DW)과 chlorogenic acid ($7.7\;\cal{mg/g}$ DW)는 IBA $5.0\;\cal{mg/L}$에 TDZ $0.1\;\cal{mg/L}$ 처리구에서 함량이 가장 높았다. Zeatin은 부정근 생육과 eleutheroside E 함량에는 크게 영향을 미치지 않으나 eleuthe-roside B와 chlorogenic acid 생산을 크게 억제하였다. The influence of different growth regulators on biomass of adventitious roots and secondary metabolites accumulation in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth of adventitious roots was observed in the presence of $2.0\;\cal{mg/L}$ IBA (6.7 g DW/L). However the productivity of eleutheroside E was not significantly different among IBA levels. High level of thidiazuron (TDZ) efficiently increased both root growth and secondary metabolites production. Especially when $0.1\;\cal{mg/L}$ TDZ was combined with $3.0\;\cal{mg/L}$ IBA, the highest eleutheroside E accumulation ($3,327{\mu}g/g $DW) was observed. When the same TDZ level was treated in combination with $5.0\;\cal{mg/L}$ IBA, both chlorogrnic acid and eleutheroside B were accumulated to maximum level. In contrast, zeatin remarkably suppressed the accumulation of both eleutheroside B and chlorogenic acid.
안진권,이위영,박영기,Ahn, Jin-Kwon,Lee, Wi-Young,Park, Young-Ki 한국산림과학회 2007 한국산림과학회지 Vol.96 No.1
This study was carried out to investigate the effect of $NO_3{^-}$ and $NH_4{^+}$ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus senticosus during 3 L-bioreactor culture. The change of medium component ratio was also measured during culture. The fresh weignt of adventitious root reached to the greatest level of 24.4g FW/L in the presence of 50 mM $NO_3{^-}$ and 10 mM $NH_4{^+}$, representing 3.4-fold increase compared to the 60 mM $NH_4{^+}$. However, as the increase of the portion of $NH_4{^+}$, the root growth was decreased. Maximum eleutheroside B and E1 production were $249{\mu}g/g$ and $43{\mu}g/g$, respectively, with 30 mM total nitrogen source. The maximum production of eleutheroside E were $788{\mu}g/g$ with 120 mM total nitrogen source. The greatest weight of adventitious root increased up to 6.2 fold of inoculum size within 9 weeks. The change of pH was influenced from 4.81 to 6.35 and the amounts of $NH_4{^+}$ and $K^+$ were decreased during culture periods. From these results we suggest, need further study of various treatment to increase the growth of biomass and the accumulation of useful secondary metabilites. 생물반응기를 이용하여 가시오갈피 부정근 증식시 부정근의 생장과 유용 이차대사산물인 eleuthroside 류 생산에 미치는 $NO_3{^-}$와 $NH_4{^+}$의 영향 및 배양기간에 따른 배지성분의 변화량을 분석하였다. 부정근 증식은 질소농도가 50 mM $NO_3{^-}$와 10 mM $NH_4{^+}$ 농도비율로 참가된 처리구에서 최대(24.4g FW/L)에 이르러 60 mM $NH_4{^+}$ 첨가에 비해서는 3.4배 증식되었다. 또한 $NH_4{^+}$의 첨가농도비율이 높아질수록 부정근의 증식은 감소하였다. Eleutheroside B와 E1은 총 질소량이 30 mM 처리에서 각각 $249{\mu}g/g$와 $43{\mu}g/g$을 생산하여 가장 우수하였으나, eleutheroside E는 총 질소농도가 높아질수록 생산량도 높아져 120 mM에서 $788{\mu}g/g$로 가장 우수하였다. 부정근 생장은 초기 접종량 대비 배양 9주만에 6.2배가 증식되었고, 배양기간별 배지 성분의 변화에서는 산도는 4.81-6.35로 변화가 비교적 심했고, $NH_4{^+}$와 $K^+$는 배양기간이 경과할수록 함유량이 낮았다. 이러한 결과로 보아 부정근의 생장과 유용이차대사산물의 함량을 높이기 위하여 다양한 처리의 연구가 수행되어야 할 것으로 생각된다.