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참옻나무(Rhus verniciflua)배발생캘러스로부터 체세포배발생에 의한 식물체 재분화
김재훈,이원석,권기원,안준교,최용익,Kim, Jae-Whune,Lee, Won-Seok,Kwon, Ki-Won,In, Jun-Gyo,Choi, Yong-Eui 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3
Excised cotyledons and embryo axises of zygotic embryos of Rhus vemicifera were cultured on Murashige and Skoog(MS) medium with various concentrations of 2,4-D. About 3-5% of explants produced callus. Embryogenic callus was preferentially induced from basal parts of embryo axis of zygotic embryos seeds when they were cultured without removal of seed coats. Somatic embryos were developed from embryogenic callus in growth regulator-free medium after 2-3 subcultures on medium with 1.0mg/L 2,4-D and these embryos were matured to cotyledonary stage. Plantlets with well-developed shoots and roots from embryos were obtained on $\frac{1}{4}$MS medium with GA$_{3}$. After acclimatization of plantlets on artificial soil, they were exposed to soil pots.