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안난희 가야대학교 보건대학원 2024 국내석사
경동맥내중막두께의 영향 인자 분석 안난희 (지도교수: 김현진, Ph.D.) 방사선학(초음파)전공, 가야대학교 보건대학원 본 연구는 2022년 5월부터 2023년 2월까지 부산 지역 일개 의료 기관을 내원한 환자들 중 경동맥 초음파 검사와 혈액검사를 모두 시행한 235명을 대상으로 후향적 분석을 시행하였다. 연구 목적은 경동맥 초음파를 통해 경동맥내중막두께와 혈류속도를 측정하고 대 상자들의 BMI 및 대사증후군 영향 인자들을 분석하여 경동맥내중 막두께에 미치는 영향에 대하여 분석하고자 하였다. 경동맥내중막두 께와 BMI의 상관관계분석 결과 p=0.004로 나타나 상관관계가 있는 것으로 확인되었다. 그 밖에 연령(p=0.009), 공복혈당(p=0.026), 음주 (p=0.006) 등이 경동맥내중막두께와 상관관계가 있는 것으로 나타났 다. 경동맥내중막두께의 증가는 심뇌혈관질환으로 이벤트의 증가를 발생시킨다. 따라서 경동맥내중막두께를 증가시키는 요인들을 배제 시키려는 노력이 반드시 필요하며 또한 다양한 예측 방법의 연구와 정기적인 검진을 통한 심혈관질환을 예방하는 것이 무엇보다 중요 하다. Key words: 경동맥내중막두께, BMI, 연령, 공복혈당, 음주
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The continued use of chemical fertilizers for crop productivity causes unexpected environmental degradation, including leaching of nitrate into ground water, surface runoff of phosphorus and nitrogen, and eutrophication of aquatic ecosystems. It has been suggested that organic farming system is a sustainable and eco-friendly approach to provide agronomic and environmental benefits particularly with regard to the improvement of soil fertility. Organic farming aims to build and maintain inherent soil fertility through the encouragement of biological processes. This means that organic ecosystems should depend on organic sources of nitrogen such as legumes, manures and liquid fertilizer. Prior studies reported the general role of soil organic matter(SOM) in nutrient storage and nutrient availability and explained how various SOM management practices can contribute to nutrient management in organic agriculture. The consequent experiments were carried out to identify the current state of liquid fertilizer use and to optimize the its application for organic farming. The first experiment was carried out to investigate the current status of liquid fertilizers used on organic and environmentally friendly farms. Component analysis of 41 types of liquid fertilizers produced and used in farms showed large differences in chemical properties among the samples. In addition, liquid fertilizers produced using the same ingredients showed differences in nutrient content, depending on the production method used by the farms. The second experiment evaluated optimal conditions for the production of agro-fishery byproduct-based liquid fertilizers. During fertilizer production, Makkolli, yogurt, dry yeast and leaf mold were added to sesame oil meal, powdered bone, fish meal, and seaweed liquid fertilizers, as microbial resources. Inorganic nitrogen concentration in the liquid fertilizer was high in the dry-yeast treatment, regardless of the ingredient used. Addition of dry yeast, which is the most suitable microbial source, during liquid fertilizer production facilitated nitrogen mineralization. The addition of molasses during liquid fertilizer production resulted in a decrease in pH as the amount of molasses increased, while EC did not vary in relation to the amount of added molasses. Inorganic nitrogen concentrations in the liquid fertilizers were seen to decrease as the amount of molasses increased. As the concentration of fish meal added to the liquid fertilizer increased, the inorganic nitrogen concentration increased as well. However, 8 weeks after fermentation, there was no difference in inorganic nitrogen concentrations between the 20% and 30% concentration with fish meal treatment. The third experiment was carried out to evaluate the effects of fish meal liquid fertilizer on cucumber growth and soil environment. There were no significant differences in cucumber plant height between the fish meal liquid fertilizer treatments and chemical fertilizer treatments. Also, the cucumber yield did not vary significantly between the concentrations of liquid fertilizers, and there were also no significant differences in the yield between the fish meal liquid and chemical fertilizer treatments. The soil microbial community varied in relation to the fish meal liquid fertilizer treatments. Microbial biomass was lower in the chemical fertilizer than in the liquid fertilizer treatment. Result of principal component analysis obtained from Ecoplate showed that fish meal liquid fertilizer treatments, no liquid fertilizer, chemical fertilizer, and no fertilizer were divided into distinct groups, with the no fertilizer treatment located furthest from the other treatments. These results show that optimization of fermentation condition would enhance the quality of liquid fertilizers and fish meal liquid fertilizer could be used as an additional nutrient source in organic farming.
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안난희 The Graduate School of Myongji University 2002 국내박사
Terminal Restriction Fragment Length Polymorphisms (T-RFLP) was analyzed for rapid analysis of bacterial community diversity in different kinds activated sludges. Analysis of activated sludge with T-RFLP revealed high bacterial diversity and 20-79 discriminable T-RFs were found in these communities. The T-RFLP patterns were numerically analyzed and clustered. The patterns derived from domestic wastewater treatment plant and leather wastewater treatment plant were found very similar. Using RDP database, we were able to predict genus names correspond to each T-RFs. To estimate the phylogenetic diversity reflected by the RFLP patterns identified the 16s rDNA clones libraries and 39 clones of Gayang, 45 clones of Songwon, and 39 clones of Hyosung were partially sequenced (approximately 500bp at the 5'end). These clones were chosen from the RFLP patterns as dominantly existing RFLP pattern among the Gayang, Songwon, and Hyosung clone libraries. The clones in each clone libraries except Gayang were classified to three divisions such as the Proteobacteria, the gram-positive group, and the Cytophaga-Flexibater-Bactrioides group. The clones in Gayang library were assigned to two more divisions (the Planctomycetes and the Acidubacterium). The bacteria belonging to these groups seemed to play a role in activated sludges. Overall, our results demonstrated that T-RFLP is a powerful method for rapidly comparing the community structure and diversity of various activated sludges, and RFLP analysis can be applied for the identification of phylogenetic distribution.
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