자외선 조사에 의해 노화된 섬유아세포에서 브라질린의 항노화 효능

심중현 한국피부과학연구원 2016 아시안뷰티화장품학술지 Vol.14 No.3

Purpose: This research was carried out to identify the skin anti-aging effect of brazilin on dermal fibroblasts. Methods: To investigate anti-aging effects of brazilin on dermal fibroblasts, we confirmed cell viability, mRNA expression, and ELISA assay (collagen, type I and MMP1). Results: In this study, we elucidated the effects of brazilin on mRNA expression (COL1A1, COL3A1, MMP1, SOD1, SOD2, SOD2, and CAT) and on protein production (collagen, type I and MMP1). Quantitative Real-time RT-PCR showed that brazilin increased mRNA expression of COL1A1, COL3A1 and SOD3 genes and protein production of collagen, type I compared with UVA-treated dermal fibroblasts. Furthermore MMP1 mRNA and protein expressions were decreased by brazilin treatment. These observations revealed that brazilin increased anti-aging effects in dermal fibroblasts. Conclusion: Therefore, we identified the anti-aging effects of brazilin, and this result showed that the brazilin can be a considerable potent ingredient for skin anti-aging. Based on this, we need to study further researches about mechanisms of brazilin to be developed for cosmetics, healthcare food, and medicine. 목적: 본 연구는 브라질린이 자외선에 의해 노화가 유도된 섬유아세포의 항노화 효과를 확인하기 위하여 수행되었다. 방법: 자외선에 의해 노화가 유도된 섬유아세포에서 브라질린의 항노화 효능을 확인하기 위해서 브라질린의 농도별 세포 생존율, mRNA 의 발현 양상, 1형 콜라겐 및 MMP1 단백질의 생성 정도를 확인하였다. 결과: 브라질린의 항노화 효과를 확인하기 위하여COL1A1, COL3A1, MMP1 의 유전자 발현을 확인한 결과, 자외선에 의해 감소한 COL1A1 과 COL3A1 유전자의 발현이 브라질린에 의해 각각 2배, 2.25배 증가하였다. 반면 자외선에 의해 증가한 MMP1 유전자의 발현은 브라질린에 의해 75% 감소하였다. 섬유아세포의 항산화효소인 SOD3 의 유전자 발현은 브라질린에 의해 23.5배 증가함을 확인하였다. 또한 1형 콜라겐 단백질의 생성이 브라질린에 의해 23% 가량 증가하였고 MMP1은 30% 감소함을 확인하였다. 따라서 본 연구자는 브라질린의 항노화 효능 및 항산화능을 확인하였다. 결론: 본 연구결과를 통하여 브라질린의 항노화 효능을 확인하였고, 향후브라질린이 화장품 및 건강식품과 의약품의 개발에 응용 될 수 있는 소재로서의 가능성을 확인하기 위해 추가적인 기전연구가필요할 것으로 생각된다.

제아잔틴에 의한 RAW264.7 세포에서의 항염효과

심중현 한국피부과학연구원 2019 아시안뷰티화장품학술지 Vol.17 No.4

Purpose: This study was performed to test the anti-inflammatory effects of zeaxanthin on skin using RAW264.7 cells. Methods: The anti-inflammatory effects of zeaxanthin on RAW264.7 cells were assessed by measuring cell viability, mRNA expression, and nitric oxide (NO)/prostaglandin E2 (PGE2). Results: The antiinflammatory effects of zeaxanthin were elucidated by analysis of IL1α/IL1β/IL6/ TNFα mRNA expression and NO/PGE2 production. Quantitative real-time polymerase chain reaction showed that zeaxanthin decreased the mRNA level of IL6 and TNFα. Additionally, PGE2/NO detection also revealed that zeaxanthin exhibited antiinflammatory properties. Conclusion: The results presented in this study suggest that zeaxanthin is an anti-inflammatory compound. Therefore, it could be a potent cosmetic ingredient with anti-inflammatory effects against atopic dermatitis. Further research on the anti-inflammatory mechanisms of zeaxanthin would not only facilitate the development of functional cosmetics but also pharmacological treatments. 목적: 본 연구는 제아잔틴에 의한 RAW264.7 세포의 항염 효과를 확인하기 위하여 수행되었다. 방법: 제아잔틴이 RAW264.7세포에 항염효과를 나타내는지 확인하기 위하여, 제아잔틴 에 의한 세포생존률 측정, 싸이토카인의 유전자 발현양상, PGE2 와 산화질소의 생성 정도를 확인하였다. 결과: 본 연구를 통해 제아잔틴이 iNOS, COX2, 인터류킨6, TNFα 유전자의 발현을 감소시킴을 확인할수 있었다. 또한 산화질소의 생성을 감소시키고 PGE2 의 생성을 감소시킴을 통해 제아잔틴의 항염효과를 확인할 수 있었다. 결론: 본 연구를 통해 제아잔틴의 항염효과를 확인하였고, 제아잔틴이 항염 및 아토피를 타겟으로 하는 화장품 원료로서의 가능성을 제시하였다. 제아잔틴이 피부미용, 화장품, 건강식품 등의 산업 등에도 응용될 수 있을 것으로 여겨지며 그 가능을 확인하기 위해 제아잔틴이 염증세포에 미치는 기전연구가 필요할 것으로 보인다.

B16F10 세포에서 Anthricin의 미백 효능

심중현 한국생약학회 2021 생약학회지 Vol.52 No.1

This study was performed to clarify the whitening effects of anthricin on the B16F10 cell line. In order to elucidate the whitening effects of anthricin on the B16F10 cell line, cell viability, messenger ribonucleic acid (mRNA) expressions, tyrosinase activity assay, and melanin production assay were measured. The effects of anthricin on tyrosinase-related protein 1(TYRP1)/TYRP2/tyrosinase (TYR)/microphthalmia-associated transcription factor (MITF) mRNA expressions and melanin content were determined. Quantitative real-time RT-PCR showed that anthricin decreased the mRNA expression level of TYRP1/TYRP2/TYR/MITF genes and melanin production contents than a-MSH-treated B16F10 cells. The tyrosinase activity assay revealed that anthricin decreased the melanin production on the B16F10 cells. These data show that anthricin increases the whitening effects on the B16F10 cells; thus, anthricin is a potent ingredient for skin whitening. Thus, further research on the mechanism of action of anthricin for the development of not only cosmetics, but also healthy food and medicine should be investigated.

6,8-Diprenylorobol의 멜라닌 합성 억제 효능

심중현 한국생약학회 2021 생약학회지 Vol.52 No.2

This study was performed to elucidated the inhibitory effects of 6,8-diprenylorobol on melanin synthesis by measuring the levels of cell viability, mRNA expression, tyrosinase activity, and melanin production in the B16F10 cell line. The effects of 6,8-diprenylorobol on tyrosinase-related protein 1 (TYRP1), TYRP2, tyrosinase (TYR), and microphthalmia-associated transcription factor (MITF) mRNA expression levels and melanin content were determined. Quantitative real-time RT-PCR shows that 6,8-diprenylorobol decreases the mRNA expression levels of TYRP1, TYRP2, TYR, and MITF in B16F10 cell line, resulting in lower levels of melanin production compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that 6,8-diprenylorobol decreases melanin production in B16F10 cells. These results demonstrate the whitening effects of 6,8-diprenylorobol on B16F10 cells; thus, 6,8-diprenylorobol is a potent ingredient for skin whitening. Further research is needed on the mechanism of action of 6,8-diprenylorobol. Such research will benefit not only cosmetics, but also the health food and medical industries.

거품돌산호 추출물의 멜라닌 합성 억제 효능

심중현 한국생약학회 2021 생약학회지 Vol.52 No.3

This study was performed to elucidate the inhibitory effects of Alveopora japonica extract on melanin synthesis by measuring the levels of cell viability, mRNA expression, tyrosinase activity, and melanin production in the B16F10 cell line. The effects of A. japonica extract on tyrosinase-related protein 1 (TYRP1), TYRP2, tyrosinase (TYR), and microphthalmia-associated transcription factor (MITF) mRNA expression levels and melanin content were determined. Quantitative real-time RT-PCR show that A. japonica extract decrease the mRNA expression levels of TYRP1, TYRP2, TYR, and MITF in B16F10 cell line, resulting in lower levels of melanin production compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that A. japonica extract decrease melanin production in B16F10 cells. These results demonstrate the whitening effects of A. japonica extract on B16F10 cells; thus, A. japonica extract is a potent ingredient for skin whitening. Further research is needed on the mechanism of action of A. japonica extract. Such research will benefit not only cosmetics, but also the health food and medical industries.

자외선 조사에 의해 노화된 인간각질형성세포에서 구멍쇠미역 추출물의 항노화 효능

심중현 한국생약학회 2021 생약학회지 Vol.52 No.4

This research was carried out to investigate the moisturizing effects of Agarum cribrosum extract on normal human epidermal keratinocytes (NHEKs). Moisturizing effects of A. cribrosum extract on NHEKs were measured by quantitative real-time RT-PCR to verify the gene expressions related to skin hydration, hyaluronic acid (HA)-ELISA to detect HA production, and cell viability assays. A. cribrosum extract increased the mRNA levels of the AQP3 and HAS2 genes and HA production in NHEKs. On the other hand, A. cribrosum extract decreased the mRNA level of the KRT1 and KRT10 genes known as differentiated keratinocyte marker in NHEKs. This research showed the moisturizing effects of A. cribrosum extract. The results indicate that A. cribrosum extract can be a potent functional ingredient for skin hydration and anti-aging products. Further study is warranted regarding the use of A. cribrosum extract to develop not only cosmetics but also food and medicine.

자외선 조사에 의해 노화된 섬유아세포에서 Cycloheterophyllin의 항노화 효능

심중현 한국생약학회 2019 생약학회지 Vol.50 No.4

This study was carried out to identify the skin anti-aging effect of cycloheterophyllin on dermal fibroblasts. To elucidate anti-aging effects of cycloheterophyllin on dermal fibroblasts, I measured cell viability, mRNA expressions, and Collagen, type I/matrix metallopeptidase 1(MMP1)-ELISA assay. In this study, I investigated the effects of cycloheterophyllin on Collagen, type I, alpha 1(COL1A1)/Collagen, type III, alpha 1(COL3A1)/MMP1/Superoxide dismutases/Catalase(CAT) mRNA expressions and Collagen, type I/MMP1 protein production. Quantitative Real-time RT-PCR showed that cycloheterophyllin increased mRNA level of COL1A1/COL3A1/CAT genes and collagen, type I protein by ELISA assay compared to UVA-treated dermal fibroblasts. Furthermore MMP1 mRNA and protein expressions were decreased by cycloheterophyllin treatment. These observations revealed that cycloheterophyllin increased anti-aging effects in dermal fibroblasts. Therefore, I identified the anti-aging effects of cycloheterophyllin, and these results showed that the cycloheterophyllin can be a considerable potent ingredient for skin anti-aging. Based on this, I anticipated further researches about cycloheterophyllin for mechanism to develop not only cosmetics but for healthcare food or medicine.

자외선 조사에 의해 노화된 인간각질형성세포에서 Artocarpin의 항노화 효능

심중현 한국생약학회 2020 생약학회지 Vol.51 No.1

The aim of this study was to investigate the epidermal moisturizing effects of artocarpin on normal human epidermal keratinocytes (NHEKs). To investigate the effects of artocarpin on NHEKs, cell viability and the expression of mRNAs related to skin hydration were measured. In addition, hyaluronic acid (HA)-ELISA assay was performed. Here, the effects of artocarpin on AQP3, HAS2, KRT1, and KRT10 mRNA expression, and on HA production, following UVA treatment were reported. The Quantitative real-time PCR results demonstrate that artocarpin increased AQP3, HAS2, KRT1, and KRT10 mRNA levels. The HA-ELISA assay revealed that artocarpin also increased HA production in NHEKs. Through these experiments, the epidermal moisturizing effects of artocarpin have been elucidated, providing evidence that artocarpin may be a potent cosmetic ingredient in skin anti-aging and moisturizing products. Based on these results, I anticipate that further research on the mechanisms of action of artocarpin may allow the development of not only cosmetics, but also medicines and healthcare foods.

자외선 조사에 의해 노화된 인간각질형성세포에서 줄의관말 추출물의 항노화 효능

심중현 한국생약학회 2023 생약학회지 Vol.54 No.3

This research was conducted to investigate the anti-aging and moisturizing effects Carpomitra costata extract on normal human epidermal keratinocytes (NHEKs). Moisturizing effects of C. costata extract on NHEKs were determined by quantitative real-time RT-PCR to identify cell viability assays, the mRNA levels associated with skin hydration, and hyaluronic acid (HA)-ELISA to detect HA production. C. costata extract increased mRNA levels of AQP3 and HAS2 genes and HA production in UVA-irradiated NHEKs. On the other hand, C. costata extract decreased the gene expressions of the FLG, KRT1 and KRT10 known as differentiated keratinocyte marker in NHEKs. This research showed the moisturizing effects of C. costata extract. The results indicate that C. costata extract can be a functional ingredient in skin moisturizing and anti-aging products. Further study is warranted regarding the use of C. costata extract to develop not only cosmeceutics but also food and medicine.

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

심중현,이태룡,신동욱 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.6

Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O2 concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.