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반응표면분석에 의한 진피 에탄올 추출물의 추출조건 최적화
정지은 ( Ji Eun Jeong ),심상필 ( Sang Phil Shim ),정유석 ( Yoo Seok Jeong ),정희경 ( Hee Kyoung Jung ),김영찬 ( Young Chan Kim ),홍주헌 ( Joo Heon Hong ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.5
This study was conducted to monitor the quality characteristics Citrus unshiu ethanolic extracts by a response surface methodology. In extraction conditions based on the central composite design with variations in ethanol concentration(0~100%), extraction temperature(35~95℃), and ratio of solvent to sample(20 mL/g). The maximum value of yield was 36.31% at 93.18℃, 67.13% of ethanol concentration. The extraction yield and total polyphenol content were improved with the increase of ethanol concentration than extraction temperature. Total flavonoid content was improved with the increase of extraction temperature than ethanol concentration. The coefficients of determinations(R2) were 0.8646(p<0.1) and 0.9153(p<0.05) in electron donating ability and hesperidin content, respectively. Estimated conditions for the maximized extraction including yield, total polyphenol content, total flavonoid content, electron donating ability, and hesperidin content were 55~73% in ethanol concentration, 89~95℃ in extraction temperature, and 20 mL/g in ratio of solvent to sample.