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자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정
모영근(Young Keun Mo),신영근(Young Keun Shin),박동진(Dong Jin Park),김창진(Chang Jin Kim),이종길(Chong Kil Lee),한성순(Seong Sun Han) 대한약학회 1995 약학회지 Vol.39 No.6
Soil microorganisms producing immunomodulators that can restore ultraviolet B (UVB) radiation-induced suppression of the immune response were screened in vitro. Exposure of freshly isolated murine epidermal cells (EC) to 180 J/m2 of UVB radiation resulted in approximately 90% impairment of accessory cell function, as measured by their ability to support anti-CD3 monoclonal antibody-induced T-cell mitogenesis. When the culture supernatants of 150 actinomycete strains were examined for their capacity to prevent or repair the UVB-induced impairment of accessory cell function, 4 of them were identified to contain immunomodulators that can restore the decreased accessory cell function. The soil isolate that showed the most effective restorative activity, G40025, was selected and further characterized. Addition of 10mcl of the culture supernatant of G4002 grown in G-media to cultures of UVB-irradiated EC right after UVB-irradiation restored the decreased accessory cell function by 58%. The immunomodulator produced by G40025 appeared to be stabled at 100oC for 10 min. Taxonomical studies by cultural, morphological, and physiological characterization showed that the soil isolate, G4002, belongs to the genus Streptomyces.
함영주,신영근,최낙진,강상모,Ham, Young-Joo,Shin, Young-Keun,Choi, Nag-Jin,Kang, Sang-Mo Korean Association of Organic Agriculture 2013 韓國有機農業學會誌 Vol.21 No.3
This study was conducted to investigate the effect of fermentation on biological activity of Chelidonium majus var asiaticum and to screen effective starter culture strains. Antibacterial activity against to Staphylococcus aureus, Listeria monocytogenes and Salmonella gallinarum and antioxidant activity as free radical scavenging activity by using DPPH were tested. Total six starter culture strains, two of Lactobacillus brevis, one of Lactobacillus plantarum and three of Saccharomyces cerevisiae were used. Plant extract was prepared after fermentation by using ethanol. All strains showed normal growth in viable cell counts of fermented cultures and L. plantarum showed the highest cell growth significantly (p<0.05). In antibacterial activities of extracts, the activity was found only in the extract from the fermentation using L. plantarum. In antioxidant activity, the highest activity was shown in the fermentation using L. plantarum significantly (P<0.05). Newly produced spots in two of three elution systems on TLC-DPPH test were detected in the fermentation using L. plantarum.