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수레쉬쿠마 水原大學校 2019 論文集 Vol.33 No.-
One of most abundant biopolymer, chitin based magnetic nanomaterials are prepared. Magnetic chitin (MCT) particles treated with dopamine followed by reaction with glutaraldehyde, can be used as solid, recoverable, cheaper and robust matrix system for enzyme immobilization.
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Preparation of Metal Nanocomposite Aerogels
수레쉬쿠마 水原大學校 2016 論文集 Vol.30 No.-
Due to high porosity and readily accessible inter-connected open pore system aerogel based materials gained a special interest in various fields. Nanomaterial incorporated aerogels are potential candidates for catalysis, thermal insulation, filter materials, energy absorbers, heat and sound insulation applications. In this work, light weight silver nanomaterial incorporated bacterial cellulose (BC) aerogel is prepared without the addition of any reducing agent. BC acts as a nanoreactor and reducing agent for metallic nanoparticle synthesis.
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Biopolymer based Antioxidant Material
수레쉬쿠마 水原大學校 2017 論文集 Vol.31 No.-
There is considerable attention in generating suitable platform to create a novel antioxidant material based on polymer. Particularly, chitosan and its derivatives can be formed into fibers, films, gels, sponges and nanoparticles gained interest as attractive biopolymers. Biocompatible, biodegradable, low toxic and polycationic polysaccharide chitosan is composed of glucosamine and N-acetyl-glucosamine linked by β(1,4) glycosidic bond, which can be derived from second most abundant polymer, chitin. Natural polyphenols are known to have strong antioxidant properties. Dopamine, a catecholamine neurotransmitter present in a wide variety of animals, can be easily auto-oxidized to form self-polymerized polydopamine (Pdop) which has similar antioxidation properties as polyphenols. A novel chitin-based antioxidant with Pdop, is proposed in this work.
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Magnetic Nanocomposite as a Mimetic Peroxidase and its Application for Glucose Assay
수레쉬쿠마 水原大學校 2014 論文集 Vol.28 No.-
This work focuses on mimic peroxidase activity of magnetic nanocomposites. Polydopamine coating methodology is introduced to create multifunctionality surface of MBC act not only acted as mimic peroxidase, but also used as enzyme immobilization matrix. Glucose oxidase (GOX) was successfully immobilized and H2O2 generated by the GOX reaction was effective removed by the mimic peroxidase activity of the nanocomposite.
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수레쉬쿠마,김인호 한국가금학회 2022 韓國家禽學會誌 Vol.49 No.1
This study was conducted to investigate the effects of dietary inclusion of phytase on the growth performance, organ weight, and tibia ash of broilers. A total of 1008 one-day-old Ross 308 broiler chicks (mixed gender) with body weight 42 ± 0.90 g (mean ± SD) were used in a trial for 32 d. Chicks were assigned to one of the two treatment diets. Each treatment consisted of 28 replicate cages, with 18 birds per cage. The dietary treatments were as follows: CON, basal diet; TRT1, basal diet + phytase 1,500 Fan Terminal Unit (FTU)/kg diet. Dietary inclusion of 1,500 FTU/kg phytase supplementation in broilers showed only slight improvements in daily feed intake (P=0.086) and feed conversion ratio (P=0.065) on day 9 compared with that in the control group. However, organ weights of the breast muscle, liver, spleen, kidney, and bursa of Fabricius were not affected by the dietary treatments. In addition, the dietary inclusion of 1,500 FTU/kg phytase supplementation in the broiler diet had no effect on tibia ash. The addition of 1,500 FTU/kg phytase in the basal diet of broilers did not have any adverse effect on growth performance, organ weight, and tibia ash, suggesting that phytase supplementation in broiler diets will exhibit comparable effects as that of corn-soybean meal-based diet.
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Biosequestration of Carbon Dioxide Using a Silicified Carbonic Anhydrase Catalyst
Liang-Jung Chien,수레쉬쿠마,Hsiao-Hsin Hsieh,Jui-Lung Wang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
Using recombinant DNA technology, we constructed a dual fusion gene expression plasmid,pRCAH-30, encoding carbonic anhydrase (CA) from the cyanobacterium Synechocystis sp. PCC6803, an R5 peptide sequence, and an affinity (His)6 tag, to allow the simultaneous purification and immobilization of the encoded fusion enzyme, termed RCAH. The expressed fusion protein was approximately 30 kDa, and could be rapidly purified using affinity resins. To enhance enzyme activity,the R5 peptide facilitated immobilization by means of silicification with tetramethoxysilane; the aggregated particles were approximately 300 nm in diameter. Activity tests revealed that the enzyme functioned optimally between pH 7.0 and 7.5; maximum stability was achieved between 25and 45oC, at pH 6.0 ~ 8.0. Activity of the fusion enzyme persisted, even after encapsulation by biomimetic silicification. In fact, silicone embedding stabilized the enzyme structure, thereby increasing its stability and reusability rate under different environmental conditions. In addition, the silicified enzyme reduced waste CO2 gas from 800 to 42 ppm, resulting in a gas capture rate of 94.7% after conversion. Thus, the construct developed in this study can be effectively utilized for the sequestration of industrial waste CO2 gas.
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닭 오브알부민 프로모터의 길이에 따른 유전자 발현 활성 및 에스트로겐 반응성 분석
양현,김경운,김점순,우제석,이휘철,최훈성,정선근,수레쉬쿠마,이해선,오건봉,변승준 한국가금학회 2019 韓國家禽學會誌 Vol.46 No.1
Chickens have been considered as well-defined animal bioreactor. The optimized ovalbumin promoter is essential for recombinant protein production in transgenic chicken. Here we try to compare the activity and identify the effect of estrogen on ovalbumin promoter according to each promoter length with estrogen response element (ERE) existence. We cloned two (2.8 and 5.5 kb) ovalbumin promoters that the 5.5 kb contained the ERE but the 2.8 kb did not, and these two promoters were cloned to pGL4.11 vector. Additionally, we constructed another pGL4.11 vector containing of the 4.4 kb (with ERE) ovalbumin promoter deleted with 1 kb between ERE region and the 2.8 kb promoter. For reporter assay, HeLa, MES-SA, LMH/2A, and cEF cells were transfected with all the pGL4.11 vectors. The comparative analysis showed that the mutated 4.4 kb promoter has more potent activity than the 2.8 and 5.5 kb promoters in HeLa, MES-SA, and LMH/2A cells. However, there is no significant difference in cEFs. Also, these cells transfected with the mutated 4.4 kb promoter were treated with the 17β -estradiol (0∼3,000 nM) and HeLa, MES-SA, and LMH/2A cells showed estrogen responsibilities, but cEFs did not. Besides, the mutated 4.4 kb promoter has still higher activity than the 2.8 and 5.5 kb promoter, and there is no transcriptional induction effect in 2.8 kb promoter at 500 nM estrogen that is blood concentration of laying hens. Hence our study strongly suggested that the mutated 4.4 kb promoter is considered as one of the most efficient length for generating transgenic chicken.
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