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L-Tryptophan투여에 의한 간내 Triglyceride 축적에 대한 실험적 연구
정남수,김제현,송정적 최신의학사 1971 最新醫學 Vol.14 No.9
In 1971 Kim observed that the injection of excess dose of tryptophan produced the accumulation of hepatic triglyceride and also reported that the mechanism was involved in fatty acid or triglyceride syn?thesis but not oxidation of fatty acid by using of labeled compound, palmitate-1-014 and acetate-1-C" We report our investigation of a minimum amount of tryptophan to produce hepatic triglyceride accu?mulation and a mechanism in vivo and in vitro experiments(in liver slice and in the isolated perfused liver). Male albino rats weighing 200-250g, prior to the experiments have been fed a diet 15% casein for one week. L-Tryptophan of 0.34, 0. 1, 0.05, 0.025, and 0.001 mmole and several metabolites of tryptophan of 0. 34 m mole per rat have been injected intraperitoneally into the intact rats. Six hours later the rats have been sacrificed, and the concentration of hepatic triglyceride has been determined. And lipolysis experiments by tryptophan in epidydimal adipose tissue was performed, and also triglyceride accumulation in the isolated perfused liver was studied. Results as follows 1. In vivo. a) An injection of 0. 34, 0. 1," 0.05, 0. 025, and 0.001mmole of tryptophan per each rat produced hepatic triglyceride accumulation of 21.80±0.87, 19.96±2.53, 16.67±1.12, 11.19±1. 10 and 10.64±0.54mg in each g of liver. b) The minimum amounts of tryptophan to produce hepatic triglyceride accumulation was 0.05mmole per each rat. c) An injection of kynurenine, 3-hydroxyanthranilic acid, nicotinic acid, or nicotinamide produced hepatic triglyceride accumulation of 13.21, 11.13± 0.54, 7.5±0.24, and 9.47±0.96mg per each g of liver, and these values were lower than 21.8±0.87 mg of tryptophan treated, and similar or lower than 12.8±1.10 mg of control. d) Hepatic triglyceride accumulation was not produced by the tryptophan metabolites such as kynure- nine, 3-hydroxyanthranilic acid, nicotinic acid, or nicotinamide. e) An injection of 11.2? mole sod. citrate had no effect on hepatic triglyceride accumulation. 2. In Vitro a) In Warburg's vessels, tryptophan had no effect on lipolysis in rat epidydirnal adipose tissue. b) In perfusion system, i) In 0, 2. 5, 4, and 5 hours following the infusion of tryptophan hepatic triglyceride contents were shown as 10.66±1.6, 13.6, 20.34, 25.2, and 21.7mg per g of liver. ii) With infusion at the rate of 1.26ml (2.02mg of tryptophan) per each hour, the amino acid preparation which contained 1, 600 mg of tryptophan per liter did not accumulate hepatic tirglyceride.
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