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벼 현탁배양세포괴로부터 식물체 재분화에 대한 Electroporation Parameter의 영향
김준철(Joon C. Kim),김명덕(Myoung D. Kim),손수인(Su I. Son) 강원대학교 기초과학연구소 1998 기초과학연구 Vol.9 No.-
Embryogenic suspension-cultured cells derived from leaf base region of the germinated rice (Oryza sativa L. cv. Nakdong) were cultured in LS221 liquid medium with 2 mg/L 2,4-D, 0.2 mg/L kinetin and 0.1 mg/L GA₃for 4 months. After four days of subculture, filtered suspension-cultured microcolonies of 100-250 μm were electroporated with 400 V/cm for 1.2ms. Electroporated cell clusters divided with cell viability of 85% on LS221liquid medium supplemented with 2mg/L 2,4-D, 0.2 mg/L kinetin and 0.1 mg/L GA₃. After three weeks of culture microcalli were formed with frequency of 70% in the dark at 27℃ on MS25 medium. Under fluorescent light of 30 ± 3 μEm-²s-¹these yellow microcalli transferred to N??-22 regeneration medium, formed green spots with frequencies of 24% and frequency of shoots produced from green spot calli was 52%.
벼(O.sativa)의 하배축 절편체로부터 유도된 배발생캘러스와 현탁배양세포의 특성
김준철(Joon C. KIM),김명덕(Myoung D. Kim),손수인(Su I. Son) 강원대학교 기초과학연구소 1998 기초과학연구 Vol.9 No.-
Frequency of germination and contamination from the seeds of 13 rice (O. sativa) cultivars was investigated. Also frequency of callus induction from the hypocotyl explants of seedling was tested on LS medium supplemented with 2.5 mg/L 2,4-D in the dark at 27℃. Hypocotyl explants of selected 4 rice (O. sativa) cultivars were cultured on LS medium supplemented with various concentrations of 2,4-D. The highest frequency of callus formation was obtained when cv. Koshihikari, cv. Nakdong, cv. Ginbuchal and cv. Ricol were cultured on medium supplemented with 2.5 mg/L, 1.0 mg/L, 1.5 mg/L and 2.0 mg/L 2,4-D respectively. Embryogenic calli of pale yellow, globular type were selected and cultured in AA₂ medium supplemented with 2.0 mg/L 2,4-D, 0.2 mg/L kinetin and 0.1 mg/L GA₃ for suspension culture. Initial suspension culture showed large elongated cells and round cells with granular cytoplasm. The fine suspension cell lines were established after 2 months of subculture.