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유우에서 포도상구균 백신과 면역증강제 투여후 우유의 체세포수 변화 및 분리된 포도상구균의 항생제 내성에 관한 조사
성명숙 ( Myoung Suk Seong ),김규섭 ( Keu Seob Kim ),김우현 ( U Hyoun Kim ),박희주 ( Hee Ju Park ),배성수 ( Sung Su Bae ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.1
Thirty-one dairy cow from two farm(more than 500,000 cells/mi of bulk milk) in Kyongbuk northern province were selected because of their high somatic cell(more than 500,000 cells/nil of milk in individual cow). Each cow received staphylococcus aureus vaccine(Labac Staph) and immunostimulant(Ultracon) by intramuscular injection to be repeated every fifteen days for 5 times. The present study was investigated variation of somatic cell after administration of Labac Staph and Ultracon, and antibiotics resistance of isolated staphylococcus spp from milk in selected cow. The results obtained through the survey were summarized as follows 1. Ten dairy cow was injected in A farm. Chronic mastitic two cow after 2rd injection was weeded out the herd. Decrease rate of somatic cell after 1st, 2nd, 3rd, 4th and 5th administration were 41.4%, 35.6%, 56.4%, 65.4% and 36.7%, respectively. Twenty-one cow was injected in B farm. Chronic mastitic five cow after 3rd injection was weeded out the herd. Decrease rate of somatic cell after 1st, 2nd, 3rd, 4th and 5th administration were 36.9%, 59.9%, 24.5%, 62.6% and 78.4%, respectively. 2. in A farm, isolated staphylococcus spp were identified as S hyicus 2 strains(11.8%), coagulase negative staphylococcus 15 stains(89.2%) and S epidermidis 6 strain(35.3%). In B farm, isolated staphylococcus spp were identified as S aureus 19 strains(55.98%) and coagulase negative staphylococcus 15 strains (44.2%). 3. In A farm, antibiotics resistant rate of isolated staphylococcus spp was high at ampicillin, penicillin and kanamycin, and middle at neomycin, streptomycin and erythromycin. In B farm, antibiotics resistant rate was moderate at ampicillin, penicillin, gentarnicin, ka- namycin, neomycin, streptomycin, erythromycin and tetracycline, and coagulase negative staphylococcus spp was moderate at streptomycin.
돼지 및 닭에서 분리된 균에 대한 봉독의 최소억제농도(MIC)
김순태 ( Soon Tae Kim ),황지영 ( Ji Young Hwang ),성명숙 ( Myoung Suk Sung ),제소양 ( So Yang Je ),배동록 ( Dong Rok Bae ),한상미 ( Sang Mi Han ),이성해 ( Sung Hae Lee ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.1
Bee venom is synthesized in the venom gland`s of worker and queen bees and stored in their venom sacs. Bee venom is a rich source of enzymes, peptides and biogenic amines. there are at least 18 active components in the venom which have some pharmaceutical properties. This study was performed to evaluate minimum inhibitory concentration(MIC) of bee venom against bacteria isolated from pigs and chickens with disease. In case of reference strains, the MIC(㎍/㎖) of Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, and Salmonella typhimurium ATCC 6538 were 64, 64 and 32, respectively. In case of bacteria isolated from pig and chicken, the MIC of Staphylococcus aureus, Staphylococcus hyicus and Staphylococcus chromogenes were 8, 128 and 128, and that of 11 strains of Escherichia coli were 8 to >512 and that of 8 strains of Salmonella spp were >512. Antibacterial resistance test of 22 strains isolated from pig and chicken and 3 reference strains were performed by agar gel diffusion method, using 17 antibacterial drugs including penicillin, cefazolin, tetracycline and quinolone group. The multiple drug resistant patterns were found in most strains isolated from pig and chicken.
경북지역 산란계에서 Avian pneumovirus에 대한 항체가 및 바이러스 검출 조사
김정은 ( Jung Eun Kim ),황지영 ( Ji Young Hwang ),배동록 ( Dong Rok Bae ),성명숙 ( Myoung Suk Sung ),김순태 ( Soon Tae Kim ),김상윤 ( Sang Yun Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
Avian pneumovirus(APV), also known as avian rhinotracheitis(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis(TRT). The aim of this study was to establish the presence or absence of antibodies to APV by an enzyme-linked immunosorbent assay(ELISA) and confirm APV by a reverse transcriptase-polymerase chain reaction(RT-PCR). The tested serum and feces were collected from laying hens in Gyeongbuk province. The positive farms with antibody against APV by ELISA were 90(96.7%) of 93 and positive serum samples were 433(93.1%) of 465 different sera. By regional group, sera from Uiseong, Cheongsong and Bonghwa were noted as 100% positive and positive rates of samples from Yeongju, Andong and Yeongyang were 93.3%, 85.7% and 50%, respectively. However, APV was not detected in feces samples by RT-PCR.