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사인추출물의 인슐린 유사 성장인자-I의 합성과 피부 노화 개선에 대한 효과
최규호 ( Gyu Ho Choi ),김수남 ( Su Nam Kim ),이소희 ( So Hee Lee ),성대석 ( Dae Seok Sung ),손의동 ( Eui Dong Son ),이창훈 ( Chang Hoon Lee ),이병곤 ( Byeong Gon Lee ),장이섭 ( Ih Seop Jang ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.3
본 연구에서 프로모터-리포터 분석방법을 통해 인슐린 유사 성장인자-I의 프로모터를 자극하는 천연물을 선별한 결과 사인 추출물이 가장 좋은 프로모터 자극효과를 나타냈으며, 무모생쥐에서 패쇄첩포 후 RT-PCR로 실험한 결과 IGF-1 mRNA를 35% 증가시키는 것으로 나타났다. 사인 추출물의 피부 주름개선 효과를 알아보기 위하여 인체 섬유아세포에서는 type-I collagen과 MMP-1 합성 변화를 관찰하였으며, 무모생쥐에서는 콜라젠의 증가와 진피 두께를 관찰하였다. 그 결과, 동위원소를 이용한 콜라젠 증가실험에서 type-I collagen은 38% 증가하였으며 무모생쥐에서 실시한 RT-PCR 결과에서는 mRNA가 21% 증가하는 것으로 관찰되었다. MMP-1 효소발현의 경우 ELISA 분석을 통해서 63%의 높은 발현저해능을 확인하였고 Western blot에서도 발현이 저해되는 것을 확인하였다 추출물을 무모생쥐에 패쇄첩포 하였을 경우 대조군에 비해 진피 두께가 두꺼워지고 콜라젠 양도 증가되는 것으로 조직염색 관찰을 통해 확인하였다. 이상의 결과를 통해 사인추출물은 피부 주름개선에 좋은 효과를 나타내는 것으로 보이며, 여기에는 인슐린 유사성장 인자-I의 발현증가와 관련된 기전이 관여하는 것으로 판단된다. We screened several materials to stimulate IGF-1 promoter activity using luciferase reporter assay and found that Amomi Semen extract (ASE) among them is the most powerful stimulator. We also studied about the anti-wrinkle effect of ethanolic extract of Amoni Semen in vitro and in vivo. Semi-quantitative RT-PCR showed that the extract elevated the presence level of IGF-1 mRNA. And [<sup>3</sup>H] proline incorporation and semi-quantitative RT-PCR showed that the extract increased the expression of type-I collagen compared with vehicle in vitro and in vivo, respectively. Significant inhibition of MMP-1 expression was determined by ELISA and Western blot. Finally, topical treatment of the extract on hairless mouse's dorsal skin expanded the volume of collagen and dermal thickness. These results suggest that Amomi Semen may be a good candidate for improving extracellular matrix through the increase of collagen expression and inhibition of MMP-1 expression. Moreover, this study enables us to guess that IGF-1 stimulated by the extract may be involved in the mechanism of anti-wrinkle effect of it.
總炭酸 濃度에 따른 pH 및 硬度가 繭層 Sericin 溶解에 미치는 影響
南永洛,蔡大錫,成載千 한국잠사학회 1989 한국잠사곤충학회지 Vol.31 No.2
Two kinds of solution for the measurement of solubilities of Seriein are prepared as followings at temperature 90 dog, C. One has the total carbonate concentration as 0, 50, 100mg CO2/l prepared with non-carbonate distilled water, sodium hydrogen carbonate and 0.1N HCI and NaOH, the other has total hardness, that is, calcium hardness or magnesium hardness as 0, 20, 50, 100 mg CaCO3/l respectively prepared with non-carbonate distilled water, calcium carbonate and magnesium oxide. Solubilities of Cocoon layer Sericin at above solution gives following results; 1. pH shows little effect on the solubility of Sericin at the non-carbonate solution but at the carbonate solution pH shows a sensitive effect on the solubility of Sericin. These means that pH controls the concentration of H2CO3, HCO3-and CO32- which prevent and promote the solution of Sericin. 2. After the cocoon layer treatment at the solution, the initial pH of 4.0, 7.0, 9.0 of the solution changed to 6.0~6.5 at the lower total carbonate solution. However in the higher total carbonate solution pH did not changed very much. This may be explained by the buffer action of carbonate. 3. The effect of the hardness on the solubility of Sericin was not found in the non-carbonate solution with the standard hardness after treatment of cocoon layer.
南永洛,蔡大錫,成載千,李龍雨,金三銀 한국잠사학회 1991 한국잠사곤충학회지 Vol.33 No.2
The features of waste water from by-product silk treatment of silk reeling process were investigated and the lipid extracted from waste water was analysed. The COD of waste water from by-product silk treatment was at the level of 605 mgO/l Total Dissolved Solid Particles 2,335 mg/l, and Total Suspended Solid Particles 2,123 mg/l. The lipid extracted from the waste water from by-product silk treatment was composed of triglyceride 76.8%, free fatty acids 12.5%, diglyceride 5.7% and free sterol 5.0%. In fatty acid composition of lipid, the content of loeic acid, linoleic and linolenic acid was 64.93%, whereas that of palmitic acid was 29.39% and stearic acid 4.93%.