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- 저자 : 문은호(Eunho L. Moon) (검색결과 1 건)
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배종환(Jong Hwan Bae),유영춘(Yung Choon Yoo),홍장희(Jang Hee Hong),문은호(Eunho L. Moon),송경식(Kyung Sik Song),이경복(Kyung Bok Lee) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.1
A modification of a colorimetric assay was used to determine the concentration of total and individual sulfated-glycosaminoglycan (GAG) in equine synovial fluid and serum with joint disease. For the identification of enzymatic active products of the equine synovial fluid, strong anion exchange-high performance liquid chromatography (SAX-HPLC) was performed. By the action of chondroitin ABC lyase, three unsaturated disaccharides, 2-acetamide-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-D-galactose (△Di-COS), 2-acetamide-2- deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose (△Di-C6S), and 2-acetamide-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose (△Di-C4S), were produced from the equine synovial fluid. Total GAG concentration of the equine synovial fluid was 0.82± 0.24 ㎎/㎖ (mean±SD) in normal horses, but it was decreased in horses with joint disease (0.17±0.12 ㎎/㎖). The concentration of keratan sulfate (KS), chondroitin sulfate (CS), and hyaluronic acid (HA) were decreased in horses with joint disease. The concentration of serum HA of normal horses was 77.00±66.14 ㎍/㎖, but it was markedly increased in joint diseases (168.50±147.50 ㎍/㎖), There appears to be some correlation between joint inflammation and serum HA levels as determined by experimental studies of animals. Irrespective of the explanation, it is clear that measurement of the level of serum HA may provide a useful marker for monitoring the onset and progression of a number of important diseases and disorders in equine.
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